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Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples

DOI :

10.3791/51906-v

July 6th, 2014

July 6th, 2014

18,784 Views

1Department of Medical Microbiology, University of Manitoba, 2Department of Community Health Sciences, University of Manitoba

The use of cytobrush sampling to collect lymphocytes and monocytes from the endocervix is a minimally invasive technique that provides samples for analysis of female genital tract immunity. In this protocol, we describe the collection of cytobrush samples and immune cell isolation for flow cytometry assays.

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Collection

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