Integrative Toolkit to Analyze Cellular Signals: Forces, Motion, Morphology, and Fluorescence

3.8K Views

14:55 min

March 5th, 2022

DOI :

10.3791/63095-v

March 5th, 2022


Transcript

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Cellular Signals

Chapters in this video

0:02

Introduction

1:46

Creation of Position List

3:57

Automated Image Acquisition for the Remaining Experiment

4:34

Setting Up Automated Data Analysis

6:08

Quantification of Hydrogel Deformation

6:37

Quantification of Cell-ECM and Cell-cell Forces

8:44

Segmentation of the Individual Cells in the Images

9:47

Mapping of the Pixel Intensities on the Cells

11:06

Mapping of the Forces and Motion on the Cells

11:51

Tracking of the Cellular Indentities across the Experiment

12:51

Generation of Time Tracks of the Assessed Cellular Properties

13:27

Generation of the Heat Maps of the Assessed Cellular Properties

13:53

Representative Results

14:30

Conclusion

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