Cell Staining for Senescence-associated (SA) Beta-Galactosidase
3:30
Quantifying SA Secretory Phenotype (SASP) Protein Levels
4:37
Results: Representative SA-beta-Galactosidase Staining and SASP Expression Analyses
5:42
Conclusion
Transcript
The overall goal of these techniques is to provide a toolbox for enabling the induction, monitoring, and quantification of cellular senescence. These methods can help answer key questions about the identification and quantification of cellular sen
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Cellular senescence, the irreversible state of cell-cycle arrest, can be induced by various cellular stresses. Here, we describe protocols to induce cellular senescence and methods to assess markers of senescence.