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An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence Images

DOI :

10.3791/4233-v

August 31st, 2012

August 31st, 2012

14,325 Views

1Medications Development, Ernest Gallo Clinic and Research Center, University of California, San Francisco , 2Clinical Pharmacology and Experimental Therapeutics, University of California, San Francisco , 3Translational Research Institute and the Institute for Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia

We developed a software platform that utilizes Imaris Neuroscience, ImarisXT and MATLAB to measure the changes in morphology of an undefined shape taken from three-dimensional confocal fluorescence of single cells. This novel approach can be used to quantify changes in cell shape following receptor activation and therefore represents a possible additional tool for drug discovery.

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Analytical Tool

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