Preparation of Stock Solutions and Gelification Mixture
2:53
Preparation of qPCR Master Mix, Gelification of the Reagents in the Reaction Vessels, and Gelified qPCR
4:22
Results: Ready to Use Gelified qPCR for Trypanosoma cruzi Epimastigotes DNA Detection
5:11
Conclusion
Transcript
The gelification protocol produces ready to use qPCR reactions that decrease the time and steps required to start a run. This protocol allows qPCR reactions to be produced and controlled for quality and large quantities at once, decreasing the cha
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The present work describes the steps for producing ready-to-use qPCR for T. cruzi DNA detection that can be pre-loaded on the reaction vessel and stored in the refrigerator for several months.