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DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

DOI :

10.3791/59737-v

October 8th, 2019

October 8th, 2019

8,438 Views

1Department of Chemistry, Ben-Gurion University of the Negev, 2Center for Genomic and Computational Biology, Department of Biostatistics and Bioinformatics, Duke University

Protein binding microarray (PBM) experiments combined with biochemical assays link the binding and catalytic properties of DNA primase, an enzyme that synthesizes RNA primers on template DNA. This method, designated as high-throughput primase profiling (HTPP), can be used to reveal DNA-binding patterns of a variety of enzymes.

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DNA Sequence Recognition

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