JoVE Logo
Faculty Resource Center

Sign In

Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei

DOI :

10.3791/58334-v

January 22nd, 2019

January 22nd, 2019

7,045 Views

1Biology Centre, Czech Academy of Science, Institute of Parasitology, 2Faculty of Science, University of South Bohemia

This protocol describes the purification of F1-ATPase from the cultured insect stage of Trypanosoma brucei. The procedure yields a highly pure, homogeneous, and active complex suitable for structural and enzymatic studies.

Tags

F1 ATPase

-- Views

Related Videos

article

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

article

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

article

Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells

article

Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana

article

Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum

article

Rapid Isolation of the Mitoribosome from HEK Cells

article

Isolation of Tissue Extracellular Vesicles from the Liver

article

Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids

article

Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo

article

Ready-To-Use qPCR for Detection of DNA from Trypanosoma cruzi or Other Pathogenic Organisms

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved