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Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei

DOI :

10.3791/58334-v

January 22nd, 2019

January 22nd, 2019

6,956 Views

1Biology Centre, Czech Academy of Science, Institute of Parasitology, 2Faculty of Science, University of South Bohemia

This protocol describes the purification of F1-ATPase from the cultured insect stage of Trypanosoma brucei. The procedure yields a highly pure, homogeneous, and active complex suitable for structural and enzymatic studies.

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