A detailed description of the standardized operation of the analyzer in the protocol can help a person who has never operated the analyzer to complete the experiments mostly important, accurate and with reliable results. Using Jove's innovative technology has advantages such as the availability of a single person detection kit, absence of liquid waste, being fast, being free for routine maintenance, and user friendly operation. One should first understand the detection principle of the analyzer, and then be familiar with several critical steps in the protocol such as blood collection, reagents lifting and so on.
To begin start the analyzer by turning the off or in power switch on the back of the analyzer to in and ensure that the indicator light of the analyzer is on. Enter username and password in the login dialogue box and click on login. The system should perform self check and start initialization automatically and display the sample analysis page.
Start the test preparation by clicking on next sample then enter the gender, name and other clinical information correctly and select the appropriate reference group. Later, click on okay to save the information. Tear the thin film of the blood cell detection module, press the entry exit warehouse button and place the blood cell detection module into the machine warehouse correctly, with its orifice facing outward.
Puncture the hemolytic reagent ceiling film with the tip of a quantitative pipette. For capillary blood collection, disinfect the left ring finger once with a cotton swab dipped in alcohol one way. After the alcohol naturally dries out, use a blood lancet to puncture the skin of the left ring finger.
Gently squeeze out the first drop of blood and wipe it with a cotton swab. Squeeze out enough blood to form a full water drop and collect five microliters of the blood sample using the capillary tube inside the quantitative pipette. For reagent mixing, insert the quantitative pipette into the hemolytic reagent and press it tightly to release the blood sample from the capillary tube.
Mix the blood in the capillary tube and hemolytic reagent by turning it upside down 15 to 20 times at a constant speed until no red blood remains in the capillary tube. Start sample analysis by opening the lid and squeezing the solution into the blood cell detection module. Next, press the entry exit warehouse button.
After the blood cell detection module enters the warehouse press the counting button. On the analyzer interface click on the okay button twice to confirm that the blood cell detection module has been taken out. On the analyzer interface click on the print button to print the test results.
To turn off the analyzer, click on the shutdown button on the analyzer interface and select yes in the dialogue box that pops up on the interface. Check that the system starts to execute the shutdown sequence. Set the off or in switch on the back of the mainframe to off after the shutdown sequence is completed.
The figure shows the scatter plot and Spearman correlation analysis of the evaluated and reference systems. The white blood cell count and granulocyte number exhibit the strongest correlation. Whereas, the lymphocyte number exhibits the weakest correlation between the evaluated and reference systems.
A Bland-Altman plot was obtained to visualize the consistency analysis. The white blood cell results detected by the evaluated system agree with the reference system. The average values of the differences in the leukocyte indexes are above zero, indicating that the test results of the evaluated system are slightly higher than those of the reference system.
During the experiment we should try our best to ensure that the blood warrant we collected is exactly 5 microliter and the in room side are completely dissolved by the hemolytic reagent.
