December 9th, 2022
•In this protocol, we describe how to prepare mammalian cells for single-cell proteomics analysis via mass spectrometry using commercially available reagents and equipment, with options for both manual and automatic pipetting.
Related Videos
MALDI Sample Preparation: the Ultra Thin Layer Method
Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry
Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis
Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions
Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
Sample Preparation for Mass Cytometry Analysis
Sample Preparation for Mass-spectrometry-based Proteomics Analysis of Ocular Microvessels
Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved