Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins
2.4K Views
•
09:09 min
•
August 17th, 2022
DOI :
August 17th, 2022
•Brieuc Chauvin*1, Koyomi Nakazawa*1, Alexandre Beber1,7, Aurélie Di Cicco1, Bassam Hajj1, François Iv2, Manos Mavrakis2, Gijsje H. Koenderink3, João T. Cabral4, Michaël Trichet5, Stéphanie Mangenot*6, Aurélie Bertin*1
1Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, Sorbonne Université, 2Institut Fresnel, CNRS UMR7249, Aix Marseille Univ, Centrale Marseille, 3Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, 4Department of Chemical Engineering, Imperial College London, 5Sorbonne Université, CNRS, Institut de Biologie Paris-Seine (IBPS), Service de microscopie électronique (IBPS-SME), 6Laboratoire Matière et Systèmes Complexes (MSC), Université Paris Cité, 7Institute of Biotechnology, Czech Academy of Sciences, BIOCEV
Explore More Videos
Chapters in this video
0:05
Introduction
0:49
Designing Wavy NOA (Norland Optical Adhesive) Replica from Wavy PDMS (Polydimethylsiloxane) Patterns
1:49
Generation of a Supported Lipid Bilayer and Protein Incubation
3:22
Sample Fixation, Sample Dehydration, Critical Point Drying
4:43
Sample Mounting and Coating
5:54
Acquire the Images Using a Field-Emission SEM (FE-SEM) Microscope
6:56
Results: Analysis of the Self-Assembly of Septins at the Lipid Membrane
8:27
Conclusion
Related Videos
12:30
Testing Visual Sensitivity to the Speed and Direction of Motion in Lizards
11.4K Views
11:50
Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution
2.0K Views
06:32
Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions
2.1K Views
08:27
A Nanobar-Supported Lipid Bilayer System for the Study of Membrane Curvature Sensing Proteins in vitro
2.7K Views
08:38
Protein Membrane Overlay Assay: A Protocol to Test Interaction Between Soluble and Insoluble Proteins in vitro
22.0K Views
12:20
Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends
14.3K Views
08:05
Visualizing Membrane Ruffle Formation using Scanning Electron Microscopy
2.5K Views
06:26
Pulling Membrane Nanotubes from Giant Unilamellar Vesicles
10.9K Views
02:07
Planar Supported Lipid Bilayer Assay: A Microscopy-Based In Vitro Technique to Investigate Septin Assembly on Biological Membrane Mimics
179 Views
02:01
Spherical Supported Lipid Bilayer Assay: A Technique to Observe the Septin Assembly on Microspheres
136 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved