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Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution

DOI :

10.3791/63871-v

11:50 min

June 23rd, 2022

June 23rd, 2022

1,397 Views

1Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, 2Institut Fresnel, CNRS UMR7249, Aix Marseille Univ, Centrale Marseille, 3Institut Curie, Université PSL, Sorbonne Université

In vitro reconstitution of cytoskeletal proteins is a vital tool to understand the basic functional properties of these proteins. The present paper describes a protocol to purify and assess the quality of recombinant septin complexes, which play a central role in cell division and migration.

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Purification

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