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Determining Binding Affinity (KD) of Radiolabeled Antibodies to Immobilized Antigens

5.8K Views

07:39 min

June 23rd, 2022

DOI :

10.3791/63927-v

June 23rd, 2022


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Binding Affinity

Chapters in this video

0:04

Introduction

0:49

Buffer Preparation

2:05

Antigen Immobilization and Blocking Nonspecific Sites with BSA

3:21

Serial Dilutions and Addition of the Radiolabeled Antibody (rAb) Solution

5:08

Wash Plates and Assay Radioactivity

6:07

Results: Determination of Binding Affinity

7:04

Conclusion

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