JoVE Logo

Sign In

An ELISA Based Binding and Competition Method to Rapidly Determine Ligand-receptor Interactions

19.0K Views

08:40 min

March 14th, 2016

DOI :

10.3791/53575-v

March 14th, 2016


Explore More Videos

ELISA

Chapters in this video

0:05

Title

1:07

Preparations and Introduction of Assays

2:14

Direct Ligand-Receptor-Interaction Assay (Direct LRA)

5:05

Competition Ligand-Receptor-Interaction Assay (Competition LRA)

6:24

Results: Binding Between INFL 1-3 Peptides and Their Receptor Sub-unit IL28RA

7:48

Conclusion

Related Videos

article

13:57

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects

29.0K Views

article

10:13

Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding

16.2K Views

article

12:38

Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction

19.7K Views

article

10:16

Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets

43.9K Views

article

13:26

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry

61.5K Views

article

02:13

SELEX-Based In Vitro Binding Assay to Identify RNA-Protein Interactions

202 Views

article

02:40

Ligand Binding Sites

12.6K Views

article

02:18

The Equilibrium Binding Constant and Binding Strength

12.7K Views

article

04:33

Competitive Binding Assay to Identify Compounds Disrupting Receptor-Ligand Interactions

78 Views

article

01:24

ELISA-Based Reporter Assay to Study the Receptor-Ligand Interaction in BW Cells

55 Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved