Demonstration of the Sequence Alignment to Predict Across Species Susceptibility Tool for Rapid Assessment of Protein Conservation

DOI :

10.3791/63970-v

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February 10th, 2023

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2,277 Views

Sara M. F. Vliet¹, Monique Hazemi², Donovan Blatz², Marissa Jensen³, Sally Mayasich⁴, Thomas R. Transue⁵, Cody Simmons⁵, Audrey Wilkinson⁵, Carlie A. LaLone⁶

¹Office of Research and Development, Center for Computational Toxicology and Exposure, Scientific Computing and Data Curation Division, U.S. Environmental Protection Agency, ²Oak Ridge Institute for Science and Education, ³Swenson College of Science and Engineering, Department of Biology, University of Minnesota Duluth, ⁴University of Wisconsin-Madison Aquatic Sciences Center, ⁵General Dynamics Information Technology, Research Triangle Park, ⁶Office of Research and Development, Center for Computational Toxicology and Exposure, Great Lakes Toxicology and Ecology Division, U.S. Environmental Protection Agency

Chapters

Here, we present a protocol to utilize the latest version of the US Environmental Protection Agency Sequence Alignment to Predict Across Species Susceptibility (SeqAPASS) tool. This protocol demonstrates the application of the online tool to rapidly analyze protein conservation and provide customizable and easily interpretable predictions of chemical susceptibility across species.

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