Accessing and Understanding the Data: SeqAPASS Level 1 and Level 2
5:54
Manipulating Data Settings and Visualizing the Data: SeqAPASS Level 1 and Level 2
7:23
Developing and Running a SeqAPASS Analysis: Level 3
8:28
Identify Critical Amino Acid Residues through a Literature Search
10:54
Visualizing Level 3 SeqAPASS Data and Interpretation of Results
13:45
Results: SeqAPASS Level 1, 2, and 3 Analyses of the Conservation of Amino Acid Residues and Level 1 and 2 Analyses of μ Opioid Receptor Conservation Across Taxonomic Groups
15:10
Conclusion
필기록
In the context of chemical safety, the SeqAPASS protocol provides a rapid and streamlined process for evaluating protein conservation across the diversity of species, for extrapolation of both toxicity and biological pathway knowledge. SeqAPASS wa
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Here, we present a protocol to utilize the latest version of the US Environmental Protection Agency Sequence Alignment to Predict Across Species Susceptibility (SeqAPASS) tool. This protocol demonstrates the application of the online tool to rapidly analyze protein conservation and provide customizable and easily interpretable predictions of chemical susceptibility across species.