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A Protocol for Computer-Based Protein Structure and Function Prediction

DOI :

10.3791/3259-v

16:41 min

November 3rd, 2011

November 3rd, 2011

67,149 Views

1Center for Computational Medicine and Bioinformatics, University of Michigan , 2Center for Bioinformatics and Department of Molecular Bioscience, University of Kansas

Guidelines for computer based structural and functional characterization of protein using the I-TASSER pipeline is described. Starting from query protein sequence, 3D models are generated using multiple threading alignments and iterative structural assembly simulations. Functional inferences are thereafter drawn based on matches to proteins with known structure and functions.

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