Results: Confirmation of RNA Purity of Cytoplasmic and Nuclear Fraction
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Conclusion
Transcript
This protocol is necessary to evaluate the different roles of defined biochemical elements in the nucleus and cytoplasm and to analyze intracellular interactions between these domains within various cell lines. This technique utilizes commonplace
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The present protocol offers an efficient and flexible method to isolate RNA from nuclear and cytoplasmic fractions using cultured cells, and then validate using qPCR. This effectively serves as a replacement for other RNA preparation kits.