Results: Patterns of -H2AX, DNA-PKcs, and Rad52 Foci in Colon Cancer Cells
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Conclusion
Transcript
This protocol provides a step-by-step procedure to detect radiation-induced foci of repair proteins by immunofluorescence staining in human colon cancer cell lines after irradiation with the neutron-mixed beam. Immunofluorescence imaging is a sens
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Radiation-induced DNA damage response activated by neutron mixed-beam used in boron neutron capture therapy (BNCT) has not been fully established. This protocol provides a step-by-step procedure to detect radiation-induced foci (RIF) of repair proteins by immunofluorescence staining in human colon cancer cell lines after irradiation with the neutron-mixed beam.