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Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells

DOI :

10.3791/61399-v

June 9th, 2020

June 9th, 2020

10,450 Views

1Nuclear Facilities Operations Department, National Centre for Nuclear Research, Sołtana 7, 05-400 Otwock, Poland

Radiation-induced DNA damage response activated by neutron mixed-beam used in boron neutron capture therapy (BNCT) has not been fully established. This protocol provides a step-by-step procedure to detect radiation-induced foci (RIF) of repair proteins by immunofluorescence staining in human colon cancer cell lines after irradiation with the neutron-mixed beam.

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Immunofluorescence Imaging

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