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Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

DOI :

10.3791/64360-v

January 20th, 2023

January 20th, 2023

1,855 Views

1Central Laboratory of Women’s Hospital, Zhejiang University School of Medicine, 2School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, 3Core Facilities, Zhejiang University School of Medicine

This protocol provides a rapid and size-specific isolation method for small extracellular vesicles by optimizing the size of the air spray nozzle, sheath fluid pressure, sample flow pressure, voltage, gain, and triggering threshold parameters.

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Flow Cytometric Sorting

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