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Fluorescence-Activated Nuclei Negative Sorting of Neurons Combined with Single Nuclei RNA Sequencing to Study the Hippocampal Neurogenic Niche

DOI :

10.3791/64369-v

8:16 min

October 20th, 2022

October 20th, 2022

1,798 Views

1The Francis Crick Institute, 2MSD R&D Innovation Centre

Presented here is a method to sequence single nuclei isolated from the mouse dentate gyrus that excludes most neurons through fluorescence-activated nuclei (FAN)-sorting. This approach generates high-quality expression profiles and facilitates the study of most other cell types represented in the niche, including scarce populations such as neural stem cells.

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Fluorescence activated Nuclei Negative Sorting

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