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Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing (DIVA-Seq)

DOI :

10.3791/58690-v

October 26th, 2018

October 26th, 2018

9,229 Views

1Cold Spring Harbor Laboratory

This protocol describes the manual sorting procedure to isolate single fluorescently labeled neurons followed by in vitro transcription-based mRNA amplification for high-depth single-cell RNA sequencing.

Tags

Single cell RNA Sequencing

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