Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing
18.6K Views
•
06:38 min
•
October 12th, 2018
DOI :
October 12th, 2018
•Kaya J.E. Matson1, Anupama Sathyamurthy1, Kory R. Johnson2, Michael C. Kelly3,4, Matthew W. Kelley3, Ariel J. Levine1
1Spinal Circuits and Plasticity Unit, National Institute of Neurological Disorders and Stroke, 2Bioinformatics Section, Information Technology Program, National Institute of Neurological Disorders and Stroke, 3Laboratory of Cochlear Development, National Institute on Deafness and Other Communication Disorders, 4Single Cell Analysis Facility, Frederick National Laboratory
Chapters in this video
0:04
Title
0:43
Preparation of the Spinal Cord
2:03
Detergent-mechanical Cell Lysis
3:25
Homogenization and Sucrose Density Gradient
5:11
Results: Representative Results of Nuclei Isolated from the Adult Mouse Spinal Cord
5:51
Conclusion
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