Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing
18.7K Views
•
06:38 min
•
October 12th, 2018
DOI :
October 12th, 2018
•Kaya J.E. Matson1, Anupama Sathyamurthy1, Kory R. Johnson2, Michael C. Kelly3,4, Matthew W. Kelley3, Ariel J. Levine1
1Spinal Circuits and Plasticity Unit, National Institute of Neurological Disorders and Stroke, 2Bioinformatics Section, Information Technology Program, National Institute of Neurological Disorders and Stroke, 3Laboratory of Cochlear Development, National Institute on Deafness and Other Communication Disorders, 4Single Cell Analysis Facility, Frederick National Laboratory
Chapters in this video
0:04
Title
0:43
Preparation of the Spinal Cord
2:03
Detergent-mechanical Cell Lysis
3:25
Homogenization and Sucrose Density Gradient
5:11
Results: Representative Results of Nuclei Isolated from the Adult Mouse Spinal Cord
5:51
Conclusion
Related Videos
06:22
Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq
12.1K Views
07:03
Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications
9.8K Views
10:03
Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution
2.5K Views
09:49
Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing
10.8K Views
08:18
Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting
3.3K Views
10:44
Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing
4.0K Views
07:00
Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method
24.4K Views
06:00
Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing
4.4K Views
09:09
Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics
5.5K Views
07:12
A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing
3.9K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved