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Stripe Assay to Study the Attractive or Repulsive Activity of a Protein Substrate Using Dissociated Hippocampal Neurons

DOI :

10.3791/54096-v

June 19th, 2016

June 19th, 2016

10,187 Views

1Anatomy and Neuroscience, Hamamatsu University School of Medicine, 2Cell and Neurobiology, Zoological Institute, Karlsruhe Institute of Technology (KIT)

Axon guidance molecules regulate neuronal migration and targeted growth-cone navigation. We present a powerful method, the stripe assay, to assess the ability of guidance molecules to attract or repulse neurons. In this protocol, we demonstrate the stripe assay by showing FLRT2's ability to repel cultured hippocampal neurons.

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