Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus (AAV) Capsid Variants

4.3K Views

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09:20 min

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October 18th, 2022

DOI :

10.3791/64389-v

October 18th, 2022

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Kleopatra Rapti¹^,², Olena Maiakovska¹^,², Jonas Becker¹^,², Joanna Szumska¹^,², Margarita Zayas¹^,², Felix Bubeck¹^,², Jixin Liu¹^,², Emma Gerstmann¹^,², Chiara Krämer¹^,², Ellen Wiedtke¹^,², Dirk Grimm¹^,²^,³^,⁴^,⁵

¹Department of Infectious Diseases/Virology, Section Viral Vector Technologies, Medical Faculty, University of Heidelberg, ²BioQuant Center, BQ0030, University of Heidelberg, ³Cluster of Excellence CellNetworks, ⁴German Center for Infection Research (DZIF), ⁵German Center for Cardiovascular Research (DZHK)

Summary

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Next generation Gene Therapy

Vector Engineering

Adeno associated Virus

AAV Capsid Variants

High throughput Screening

Therapeutic Transgenes

Gene Delivery Vehicles

Statistical Data

Chapters in this video

0:05

Introduction

1:16

Analysis of the AAV Viral Library (In Vitro) by NGS

3:42

Analysis of AAV Viral Library (In Vivo) by NGS

7:08

Results: Peptide Display AAV Capsid Engineering and Barcoded AAV Library Screening for Isolation of Next-Generation Gene Therapy Vectors

8:36

Conclusion

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