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1.3K Views
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09:51 min
January 27th, 2023
DOI :
10.3791/64703-v
Chapters
0:05
Introduction
0:58
Preparation of Hepatocyte Differentiation Media
1:27
Culturing Hepatic Cells Differentiated from Blood-Derived Pluripotent Stem Cells (BD-PSCs)
2:14
3D Spheroid Hepatic Differentiation
3:34
Immunofluorescence Analysis of Newly Generated 2D Liver Cell Cultures
4:59
Live Staining of Newly Formed Liver Spheroids
6:01
Examination of Spheroids Using a Fluorescence Microscope
7:32
Results: Differentiation of Human BD-PSCs into Endoderm/Hepatic Progenitor Cells and Hepatocytes
9:11
Conclusion
Transcript
使用自体来源生成人肝球体可以为毒理学、癌症和药物发现等各种研究领域做出贡献。该技术的主要优点是使用BDPC衍生的人肝细胞来设计人肝球,从而规避了原代人肝细胞或PHH的短缺。自体人球体可以扩展到再生医学和治疗,特别是在急性肝衰竭患者中。
演示该程序的将是我实验室的项目负责人Anne-Katherin Schott博士。首先准备500毫升成肝细胞敲除血清替代二甲基亚砜或KSR DMSO培养基和肝细胞成熟培养基。从原液中分装培养基,并加入新鲜的肝细胞生长因子或HGF和oncostat
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Summary
在这里,我们提出了一种使用从稳态外周血分离的单核细胞产生人类自体肝球的非遗传方法。
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