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Real-Time Quantification of the Effects of IS200/IS605 Family-Associated TnpB on Transposon Activity

DOI :

10.3791/64825-v

January 20th, 2023

January 20th, 2023

2,110 Views

1Department of Physics and Astronomy, University of California, 2Microbiology Program, University of California, 3Biophysics Program, University of California

A protocol is outlined to perform live real-time imaging to quantify how the accessory protein TnpB affects the dynamics of transposition in individual live Escherichia coli cells.

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