November 29th, 2014
•Surface Plasmon Resonance (SPR) is a label-free method for detecting bio-molecular interactions in real time. Herein, a protocol for a membrane protein:receptor interaction experiment is described, while discussing the pros and cons of the technique.
Tags
Related Videos
Quantitative Real-Time PCR using the Thermo Scientific Solaris qPCR Assay
Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays
Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR
Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer
Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions
Real-time Analyses of Retinol Transport by the Membrane Receptor of Plasma Retinol Binding Protein
Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer
Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy
Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples
FLIM-FRET Measurements of Protein-Protein Interactions in Live Bacteria.
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved