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MEDIA_ELEMENT_ERROR: Format error

A Simple Protocol for Mapping the Plant Root System Architecture Traits

2.2K Views

11:09 min

February 10th, 2023

February 10th, 2023

2,196 Views

0:04

Introduction

0:52

Arabidopsis Seed Surface Sterilization

2:23

Setting a Hydroponic System for Seed Germination

3:39

Examination of Root System Architecture (RSA)

4:07

Recording Images for Root System Architecture (RSA)

7:45

Results: Mapping the Plant Root System Architecture Traits

10:00

Conclusion

Transcript

This is a non-invasive hydroponic method to measure the root system architecture using routine lab equipments. This protocol allows the complete visualization of the plant's entire root system by manually spreading it. One of the main advantages o

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We use simple laboratory tools to examine the root system architecture (RSA) of Arabidopsis and Medicago. The plantlets are grown hydroponically over mesh and spread using an art brush to reveal the RSA. Images are taken using scanning or a high-resolution camera, then analyzed with ImageJ to map traits.

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