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High-Speed Magnetic Tweezers for Nanomechanical Measurements on Force-Sensitive Elements

DOI :

10.3791/65137-v

May 12th, 2023

May 12th, 2023

1,376 Views

1Department of Physics, Pohang University of Science and Technology (POSTECH), 2School of Biological Sciences, Seoul National University, 3Institute for Molecular Biology and Genetics, Seoul National University, 4School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH)

Here, we describe a high-speed magnetic tweezer setup that performs nanomechanical measurements on force-sensitive biomolecules at the maximum rate of 1.2 kHz. We introduce its application to DNA hairpins and SNARE complexes as model systems, but it will be also applicable to other molecules involved in mechanobiological events.

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Keywords Magnetic Tweezers

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