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NMR-Based Fragment Screening in a Minimum Sample but Maximum Automation Mode

DOI :

10.3791/62262-v

9:19 min

June 4th, 2021

June 4th, 2021

2,435 Views

1Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe-University Frankfurt, 2German Cancer Consortium (DKTK) and DKFZ

Fragment-based screening by NMR is a robust method to rapidly identify small molecule binders to biomacromolecules (DNA, RNA, or proteins). Protocols describing automation-based sample preparation, NMR experiments & acquisition conditions, and analysis workflows are presented. The technique allows for optimal exploitation of both 1H and 19F NMR-active nuclei for detection.

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NMR based Fragment Screening

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