JoVE Logo
Faculty Resource Center

Sign In

Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens

DOI :

10.3791/61780-v

February 24th, 2021

February 24th, 2021

2,809 Views

1Nuclear Magnetic Resonance Group, National Institute of Environmental Health Sciences

This protocol describes the removal of endogenous lipids from allergens, and their replacement with user-specified ligands through reverse-phase HPLC coupled with thermal annealing. 31P-NMR and circular dichroism allow for the rapid confirmation of ligand removal/loading, and the recovery of native allergen structure.

Tags

Keywords Endogenous Ligands

-- Views

Related Videos

article

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

article

Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells

article

Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve

article

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

article

Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions

article

Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins

article

Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins

article

Looking Outwards: Isolation of Cyanobacterial Released Carbohydrate Polymers and Proteins

article

Transverse Sectioning of Mature Rice (Oryza sativa L.) Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support

article

Identifying the Binding Proteins of Small Ligands with the Differential Radial Capillary Action of Ligand Assay (DRaCALA)

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved