JoVE Logo
Faculty Resource Center

Sign In

Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery

DOI :

10.3791/62469-v

6:26 min

May 16th, 2021

May 16th, 2021

3,844 Views

1Oncode Institute and Division of Biochemistry, the Netherlands Cancer Institute, 2Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe-University

Monitoring changes in the melting temperature of a target protein (i.e., thermal shift assay, TSA) is an efficient method for screening fragment libraries of a few hundred compounds. We present a TSA protocol implementing robotics-assisted nano-Differential Scanning Fluorimetry (nano-DSF) for monitoring intrinsic tryptophan fluorescence and light back-scattering for fragment screening.

Tags

Nano Differential Scanning Fluorimetry

-- Views

Related Videos

article

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

article

Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications

article

High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution

article

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

article

Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals

article

Analysis of Fluorescent-Stained Lipid Droplets with 3D Reconstruction for Hepatic Steatosis Assessment

article

Author Spotlight: Peptidome Extraction from Small Extracellular Vesicles Isolated from Bone Marrow-Derived Macrophages

article

Author Spotlight: Advancing Biotherapeutic Mass Calculation by Introducing mAbScale, a Python-Based Desktop Application

article

Author Spotlight: An Alternative Approach to Protein Quantification by Bradford Assay Using a Smartphone

article

Author Spotlight: Deciphering the Mysteries of Skeletal Muscle Fiber Types Using the MyDoBID Technique

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved