Isolation and Cryopreservation of Highly Viable Human Peripheral Blood Mononuclear Cells From Whole Blood: A Guide for Beginners
2.9K Views
•
10:48 min
•
October 25th, 2024
DOI :
October 25th, 2024
•Brian Dinh1, Marten A. Hoeksema2, Nathanael J. Spann3, Jacob Rendler1, Isidoro Cobo4,5, Christopher K. Glass3,6, Calvin Yeang1
1Division of Cardiology, Department of Medicine, University of California San Diego, 2Department of Medical Biochemistry, Amsterdam Immunity and Infection: Inflammatory diseases; Amsterdam Cardiovascular Sciences: Atherosclerosis and Ischemic Syndrome, Amsterdam UMC location University of Amsterdam, 3Department of Cellular and Molecular Medicine, University of California San Diego, 4Division of Clinical Immunology & Rheumatology, Department of Medicine, Heersink School of Medicine, University of Alabama at Birmingham, 5Comprehensive Arthritis, Musculoskeletal, Bone and Autoimmunity Center, Heersink School of Medicine, University of Alabama at Birmingham, 6Department of Medicine, University of California San Diego
Chapters in this video
0:08
Introduction
1:02
Processing & Cryopreservation of PBMCs
5:12
Processing of Plasma
6:17
Thawing PBMCs
8:15
Representative Results
10:13
Discussion
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