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Optimized Protocol for Retinal Wholemount Preparation for Imaging and Immunohistochemistry

23.0K Views

08:32 min

December 13th, 2013

DOI :

10.3791/51018-v

December 13th, 2013


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Retinal Wholemount

Chapters in this video

0:05

Title

1:38

Dissection of Wholemount Retina

2:50

Mounting onto Hydrophilized PTFE Membrane Insert

3:43

Bolus Injection of Isolectin Alexa 488

5:05

Vertical Sectioning of Membrane-mounted Tissue

6:45

Results: Hydrophilized PTFE Membrane for Wholemount Retina

8:04

Conclusion

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