Isolation of Human Primary Valve Cells for In vitro Disease Modeling
2.6K Views
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07:31 min
•
April 16th, 2021
DOI :
April 16th, 2021
•Rolando A. Cuevas1, Claire C. Chu1, William J. Moorhead III1, Ryan Wong1, Ibrahim Sultan2, Cynthia St. Hilaire1,3
1Division of Cardiology, Department of Medicine, and the Pittsburgh Heart, Lung, and Blood Vascular Medicine Institute, University of Pittsburgh, 2Division of Cardiac Surgery, Department of Cardiothoracic Surgery, University of Pittsburgh and Heart and Vascular Institute, University of Pittsburgh Medical Center, 3Department of Bioengineering, University of Pittsburgh
Chapters in this video
0:04
Introduction
0:34
Tissue Preparation and Processing
1:32
Valve Endothelial Cell (VEC) Isolation, Expansion, and Confirmation
4:01
Valve Interstitial Cell (VIC) Isolation, Expansion, and Confirmation
5:40
Results: Analysis of Isolated Human Primary Valve Cells
6:47
Conclusion
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