JoVE Logo
Faculty Resource Center

Sign In

Isolation of Human Primary Valve Cells for In vitro Disease Modeling

DOI :

10.3791/62439-v

April 16th, 2021

April 16th, 2021

2,212 Views

1Division of Cardiology, Department of Medicine, and the Pittsburgh Heart, Lung, and Blood Vascular Medicine Institute, University of Pittsburgh, 2Division of Cardiac Surgery, Department of Cardiothoracic Surgery, University of Pittsburgh and Heart and Vascular Institute, University of Pittsburgh Medical Center, 3Department of Bioengineering, University of Pittsburgh

This protocol describes the collection of human aortic valves extracted during surgical aortic valve replacement procedures or from cadaveric tissue, and the subsequent isolation, expansion, and characterization of patient specific primary valve endothelial and interstitial cells. Included are important details regarding the processes needed to ensure cell viability and phenotype specificity.

Tags

Isolation

-- Views

Related Videos

article

Isolation and Primary Culture of Rat Hepatic Cells

article

Isolation of Immune Cells from Primary Tumors

article

Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA

article

Naïve Adult Stem Cells Isolation from Primary Human Fibroblast Cultures

article

In Vivo Modeling of the Morbid Human Genome using Danio rerio

article

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

article

Isolation of Murine Valve Endothelial Cells

article

Isolation and Primary Culture of Mouse Aortic Endothelial Cells

article

Protocol for Isolation of Primary Human Hepatocytes and Corresponding Major Populations of Non-parenchymal Liver Cells

article

Isolation of Mouse Interstitial Valve Cells to Study the Calcification of the Aortic Valve In Vitro

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved