JoVE Logo
Faculty Resource Center

Sign In

Isolation of Human Primary Valve Cells for In vitro Disease Modeling

DOI :

10.3791/62439-v

April 16th, 2021

April 16th, 2021

2,096 Views

1Division of Cardiology, Department of Medicine, and the Pittsburgh Heart, Lung, and Blood Vascular Medicine Institute, University of Pittsburgh, 2Division of Cardiac Surgery, Department of Cardiothoracic Surgery, University of Pittsburgh and Heart and Vascular Institute, University of Pittsburgh Medical Center, 3Department of Bioengineering, University of Pittsburgh

This protocol describes the collection of human aortic valves extracted during surgical aortic valve replacement procedures or from cadaveric tissue, and the subsequent isolation, expansion, and characterization of patient specific primary valve endothelial and interstitial cells. Included are important details regarding the processes needed to ensure cell viability and phenotype specificity.

-- Views

Related Videos

article

Isolation and Culture of Primary Synovial Macrophages and Fibroblasts from Murine Arthritis Tissue

article

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro

article

Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats

article

The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research

article

Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells

article

Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

article

Detection of Mitophagy in Caenorhabditis elegans and Mammalian Cells Using Organelle-Specific Dyes

article

Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids

article

Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification

article

Author Spotlight: Exploring Cellular Zinc Regulation Through ZnT1 Functionality

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved