JoVE Table of Contents: Issue 219, May 2025

A Real-Time Interactive System for Studying Confrontational Pursuit Behavior in Rodents

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Journal (Behavior)

Developing a Rat Model for Bipolar Disorder

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Journal (Behavior)

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Methods Collections

Structural Biology and Analytical Chemistry Approaches for Characterizing <em>C</em>-Glycoside Metabolic Enzymes in Human Gut Microbiota

Structural Biology and Analytical Chemistry Approaches for Characterizing C-Glycoside Metabolic Enzymes in Human Gut Microbiota

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Journal (Biochemistry)

Analysis of the Expression and Complexes Assembly of the Mitochondrial Respiratory Chain Proteins in the Fission Yeast <em>Schizosaccharomyces pombe</em>

Analysis of the Expression and Complexes Assembly of the Mitochondrial Respiratory Chain Proteins in the Fission Yeast Schizosaccharomyces pombe

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Journal (Biochemistry)

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Methods Collections

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

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Journal (Biochemistry)

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Methods Collections

An Efficient Protocol to Assess ERK Activity Modulation in Early Zebrafish Noonan Syndrome Models via Live FRET Microscopy and Immunofluorescence

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Journal (Biochemistry)

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Methods Collections

Rapid Whole-Mount High-Resolution Imaging of Small Animal Vasculature for Quantitative Studies

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Journal (Bioengineering)

Enhanced Gene Delivery and Expression using Intraosseous Injection of Chitosan Nanoparticles Encapsulated Adenine Base Editor Plasmids

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Journal (Bioengineering)

Implantation of Tissue-Engineered Vascular Graft in Mouse Carotid Artery <em>via</em> Cuff Technique

Implantation of Tissue-Engineered Vascular Graft in Mouse Carotid Artery via Cuff Technique

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Journal (Bioengineering)

Development of a Benchtop Model for Evaluating the Compatibility of Wound Dressing Materials with Negative Pressure Wound Therapy Systems

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Journal (Bioengineering)

Generating Primary Cultures for Keratinocyte Live Cell Imaging

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Journal (Bioengineering)

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Methods Collections

Dynamic Multiparameter Platelet Function Assessment Using a Capacitive Biosensor

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Journal (Bioengineering)

Identification of Pre-weanling 7-Day-Old Mice with Pinna Edge Biopsies

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Journal (Biology)

Preparation of Intact Tissue for Microscopic Analysis of the Endosperm Cell Layer in Developing and Mature <em>Arabidopsis</em> Seeds

Preparation of Intact Tissue for Microscopic Analysis of the Endosperm Cell Layer in Developing and Mature Arabidopsis Seeds

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Journal (Biology)

Isolation and Culture of Primary Cholangiocytes from Mice with Polycystic Liver Disease Using a Two-step Digestion Method

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Journal (Biology)

Harmonic Radar Tags for Insect Tracking: Lightweight, Low-cost, and Accessible

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Journal (Biology)

Non-Viral Engineering of Primary Human T Cells <em>via</em> Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

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Journal (Biology)

Oropharyngeal Administration of Bleomycin in the Murine Model of Pulmonary Fibrosis

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Journal (Biology)

Application of a Novel Hyaluronan Hydrogel for Three-Dimensional Follicle Culture and Methodology for Mouse Ovarian Follicle Cryopreservation

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Journal (Biology)

Isolated Pancreatic Islet Treatment and Apoptosis Measurement

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Journal (Biology)

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

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Journal (Biology)

Postmortem Diagnosis of Rabies in Animals by the Updated, Multiplexed LN34 Real-Time Reverse Transcription-Polymerase Chain Reaction Assay

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Journal (Biology)

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Methods Collections

Generation of Functional Endodermal Hepatic Organoids

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Journal (Biology)

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Methods Collections

Minimally Invasive Cisterna Magna Injection Model for Leptomeningeal Metastasis Studies in Mice

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Journal (Cancer Research)

Live-Cell Förster Resonance Energy Transfer Imaging of Metabolically Regulated Akt Activation Dynamics in HepG2 Cells

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Journal (Cancer Research)

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

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Journal (Cancer Research)

Rapid <em>in vivo</em> Drug Response Prediction Using Leukemia Cell Grafts in Zebrafish Embryos

Rapid in vivo Drug Response Prediction Using Leukemia Cell Grafts in Zebrafish Embryos

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Journal (Cancer Research)

Laparoscopic Anatomical Hepatectomy Using Takasaki's Approach and Indocyanine Green Fluorescence Navigation

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Journal (Cancer Research)

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Methods Collections

Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells

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Journal (Cancer Research)

Plasmonic Photothermal Cancer Therapy: Nanoparticle-embedded Tumor-tissue-mimicking Phantoms for Visualizing Photothermal Temperature Distribution

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Journal (Cancer Research)

A Protocol for Explant Cultures of IDH1-mutant Diffuse Low-grade Gliomas

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Journal (Cancer Research)

Identifying PD-1/PD-L1 Inhibitors with Surface Plasmon Resonance Technology

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Journal (Cancer Research)

Evaluation of Abnormal Growth-related Genes of Hematopoietic Stem and Progenitor Cells by Combining CRISPR/Cas9 Technology with Cell Counting

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Journal (Cancer Research)

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Methods Collections

Establishment of Orthotopic Patient-derived Xenograft Models for Brain Tumors using a Stereotaxic Device

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Journal (Cancer Research)

Fluid-cell Raman Spectroscopy for <em>operando</em> Studies of Reaction and Transport Phenomena during Silicate Glass Corrosion

Fluid-cell Raman Spectroscopy for operando Studies of Reaction and Transport Phenomena during Silicate Glass Corrosion

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Journal (Chemistry)

Quantitative Structure-Activity Relationship, Activity Prediction, and Molecular Dynamics of Non-nucleotide Reverse Transcriptase Inhibitors

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Journal (Chemistry)

High-Resolution <em>C. elegans</em> Imaging Across All Larval Stages

High-Resolution C. elegans Imaging Across All Larval Stages

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Journal (Developmental Biology)

Spatially Compact Arrangement of Larval Zebrafish Sections for Spatial Transcriptomic Analysis

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Journal (Developmental Biology)

Rapid Depletion of Renal Macrophages using Human CD59/Intermedilysin Cell Ablation Tool

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Journal (Developmental Biology)

Using Laser Scanning Microscopy to Determine Electromigration in Molybdenum Disilicide

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Journal (Engineering)

Fabrication of High-Quality Whispering Gallery Mode Microbubble Resonators

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Journal (Engineering)

Mesocosm-Scale Constructed Wetland Design for Wastewater Treatment

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Journal (Environment)

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Methods Collections

Rapid Detection of Fecal Antigen of <em>Helicobacter pylori</em> Infection Based on Double Antibody Sandwich Detection Technology

Rapid Detection of Fecal Antigen of Helicobacter pylori Infection Based on Double Antibody Sandwich Detection Technology

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Journal (Immunology and Infection)

Cell-Free Dot Blot as a Practical and Adaptable Immunoassay Platform for the Detection of Antibody Response in Human and Animal Sera

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Journal (Immunology and Infection)

Detection of <em>Helicobacter pylori</em> Infection and Antibiotic Resistance via Stool Quantitative Polymerase Chain Reaction Analysis

Detection of Helicobacter pylori Infection and Antibiotic Resistance via Stool Quantitative Polymerase Chain Reaction Analysis

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Journal (Immunology and Infection)

Isolation, Fixation and Characterization of Juvenile Gilthead Seabream Head Kidney Leukocytes by Flow Cytometry

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Journal (Immunology and Infection)

Three-dimensional Location Approach with Silk Thread Guided Laparoscopic Segmentectomy for Liver Tumor

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Journal (Medicine)

Preventing Hemorrhage After Endoscopic Submucosal Dissection Using Polysaccharide Hemostatic Materials

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Journal (Medicine)

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation

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Journal (Medicine)

Novel Triple-Loop Technique for Suturing TFCC Injuries without Transosseous Tunnel

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Journal (Medicine)

Laparoscopic-Assisted Seldinger Technique for Peritoneal Dialysis Catheter Insertion

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Journal (Medicine)

Mechanism of Shu-Mu Brain-Kidney Acupoint in Treating Oligoasthenospermia in Rats <em>via</em> the Hypothalamic-Pituitary-Testicular Axis

Mechanism of Shu-Mu Brain-Kidney Acupoint in Treating Oligoasthenospermia in Rats via the Hypothalamic-Pituitary-Testicular Axis

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Journal (Medicine)

Using a 1064-nm Picosecond Neodymium-Doped Yttrium Aluminum Garnet Laser for Periorbital Hyperpigmentation

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Journal (Medicine)

Vascularized Composite Hand Allograft Procurement and Preparation for Distal and Proximal Forearm Allotransplantation: A Stepwise Approach

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Journal (Medicine)

Establishment and Validation of a Rat Model of Pulmonary Arterial Hypertension Associated with Pulmonary Fibrosis

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Journal (Medicine)

Laparoscopic Anatomical Liver Segment VII Resection with Liver Parenchymal Transection Following a Priority Approach

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Journal (Medicine)

Endoscopic Approach for Colloid Cyst Resection

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Journal (Medicine)

Reinforcement Reconstruction using Fascia Lata Autograft for Rhomboid Paralysis-Induced Scapular Winging

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Journal (Medicine)

Murine Model of Thoracic Aortic Dissection Induced by Oral β-Aminopropionitrile and Subcutaneous Angiotensin II Infusion

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Journal (Medicine)

Demystifying Venous Excess Ultrasound (VExUS): Image Acquisition and Interpretation

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Journal (Medicine)

Chinese Herbal Retention Enema for the Treatment of Ulcerative Colitis

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Journal (Medicine)

Studying Copper Nanoparticle-Induced Programmed Cell Death in Bacteria

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Journal (Medicine)

Dermoscopy Aids in the Diagnosis of Discoid Lupus Erythematosus

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Journal (Medicine)

Preliminary Study on Acupuncture Combined with Grain-sized Moxibustion for Treating Rheumatoid Arthritis with Finger Joint Pain

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Journal (Medicine)

Evaluation of Lung Function in an <em>Ex vivo</em> Murine Model

Evaluation of Lung Function in an Ex vivo Murine Model

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Journal (Medicine)

Three-Dimensional Reconstruction of Orbital Fractures

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Journal (Medicine)

A Rehabilitation Program of Exoskeleton-assisted Body Weight-Supported Treadmill Training with Non-immersive Virtual Reality for Stroke Patients

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Journal (Medicine)

Anterior Capsular Reconstruction with Human Dermal Allograft for Irreparable Subscapularis Tears

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Journal (Medicine)

Isolation of Primary Human Proximal Tubule Epithelial Cells and Their Use in Creating a Microphysiological Model of the Renal Proximal Tubule

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Journal (Medicine)

Laparoscopic S7 Hepatectomy with Positive Fluorescence Staining

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Journal (Medicine)

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Methods Collections

Mouse Model of Coronary Collateral Growth Through Repetitive Ischemia

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Journal (Medicine)

Dermal Allograft and Biceps Superior Capsule Reconstruction for Massive Irreparable Rotator Cuff Tears

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Journal (Medicine)

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Methods Collections

Kidney Procurement in a Preclinical Large Animal Model

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Journal (Medicine)

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Methods Collections

Laparoscopic Anatomical Resection of The Right Anterior Lobe Based on The Laennec Capsule Technique

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Journal (Medicine)

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Methods Collections

Laparoscopic Common Bile Duct Exploration Followed by Primary Suture Using a Modified Bile Duct Incision

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Journal (Medicine)

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Methods Collections

Fluorescence-Guided Matrix-assisted Laser Desorption/Ionization with Laser-Induced Postionization Mass Spectrometry of Individual Rat Neural Cells

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Journal (Neuroscience)

Widespread Transduction of Mouse Neocortical Neurons by Subarachnoid Injection of AAV2

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Journal (Neuroscience)

Ocular Therapeutic Delivery and Advanced Tissue Retrieval in Adult Rats

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Journal (Neuroscience)

Studying the Coding Profiles of Somatic Stimulation on Cardiac-locked Neuronal Responses in the Rat Spinal Dorsal Horn

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Journal (Neuroscience)

Multiscale Investigations of Cortical Processing by Integrating Laminar Polytrodes and Optogenetics with Micro Electrocorticography in Rodents

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Journal (Neuroscience)

Localizing Function-specific Targets for Transcranial Magnetic Stimulation in the Absence of Navigation Equipment

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Journal (Neuroscience)

Assessing Iron Deposition in the Brains of 5xFAD Mice by Perls'/DAB Staining

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Journal (Neuroscience)

Lumbar Intrathecal Injection of Gene Therapy Vectors for Central Nervous System Targeting in Mice and Rats

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Journal (Neuroscience)

Advanced Glycation End-Products Sensitize Human Sensory-Like Neuron Cells to Capsaicin-Induced Calcium Influx

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Journal (Neuroscience)

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Methods Collections

Chemogenetic Regulation in Reprogrammed Stem Cell-derived Precursor Cells in Treating Neurodegenerative Diseases

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Journal (Neuroscience)

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Methods Collections

Multiplex Detection of Gene Expression in the Intact <em>Drosophila</em> Brain Using Expansion-Assisted Iterative Fluorescence <em>In Situ</em> Hybridization

Multiplex Detection of Gene Expression in the Intact Drosophila Brain Using Expansion-Assisted Iterative Fluorescence In Situ Hybridization

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Journal (Neuroscience)

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Methods Collections

Table of Contents

Archive: Issue 219, May 2025

JUMP TO :

A Real-Time Interactive System for Studying Confrontational Pursuit Behavior in Rodents

Developing a Rat Model for Bipolar Disorder

Structural Biology and Analytical Chemistry Approaches for Characterizing C-Glycoside Metabolic Enzymes in Human Gut Microbiota

Analysis of the Expression and Complexes Assembly of the Mitochondrial Respiratory Chain Proteins in the Fission Yeast Schizosaccharomyces pombe

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

An Efficient Protocol to Assess ERK Activity Modulation in Early Zebrafish Noonan Syndrome Models via Live FRET Microscopy and Immunofluorescence

Rapid Whole-Mount High-Resolution Imaging of Small Animal Vasculature for Quantitative Studies

Enhanced Gene Delivery and Expression using Intraosseous Injection of Chitosan Nanoparticles Encapsulated Adenine Base Editor Plasmids

Wireless Telemetry Device Implantation in a Fontan Ovine Model for Continuous and Long-Term Hemodynamic Monitoring

Implantation of Tissue-Engineered Vascular Graft in Mouse Carotid Artery via Cuff Technique

Development of a Benchtop Model for Evaluating the Compatibility of Wound Dressing Materials with Negative Pressure Wound Therapy Systems

Generating Primary Cultures for Keratinocyte Live Cell Imaging

Dynamic Multiparameter Platelet Function Assessment Using a Capacitive Biosensor

Identification of Pre-weanling 7-Day-Old Mice with Pinna Edge Biopsies

Preparation of Intact Tissue for Microscopic Analysis of the Endosperm Cell Layer in Developing and Mature Arabidopsis Seeds

Isolation and Culture of Primary Cholangiocytes from Mice with Polycystic Liver Disease Using a Two-step Digestion Method

Harmonic Radar Tags for Insect Tracking: Lightweight, Low-cost, and Accessible

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Oropharyngeal Administration of Bleomycin in the Murine Model of Pulmonary Fibrosis

Application of a Novel Hyaluronan Hydrogel for Three-Dimensional Follicle Culture and Methodology for Mouse Ovarian Follicle Cryopreservation

Isolated Pancreatic Islet Treatment and Apoptosis Measurement

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Postmortem Diagnosis of Rabies in Animals by the Updated, Multiplexed LN34 Real-Time Reverse Transcription-Polymerase Chain Reaction Assay

Generation of Functional Endodermal Hepatic Organoids

Minimally Invasive Cisterna Magna Injection Model for Leptomeningeal Metastasis Studies in Mice

Live-Cell Förster Resonance Energy Transfer Imaging of Metabolically Regulated Akt Activation Dynamics in HepG2 Cells

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Rapid in vivo Drug Response Prediction Using Leukemia Cell Grafts in Zebrafish Embryos

Laparoscopic Anatomical Hepatectomy Using Takasaki's Approach and Indocyanine Green Fluorescence Navigation

Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells

Plasmonic Photothermal Cancer Therapy: Nanoparticle-embedded Tumor-tissue-mimicking Phantoms for Visualizing Photothermal Temperature Distribution

A Protocol for Explant Cultures of IDH1-mutant Diffuse Low-grade Gliomas

Identifying PD-1/PD-L1 Inhibitors with Surface Plasmon Resonance Technology

Evaluation of Abnormal Growth-related Genes of Hematopoietic Stem and Progenitor Cells by Combining CRISPR/Cas9 Technology with Cell Counting

Establishment of Orthotopic Patient-derived Xenograft Models for Brain Tumors using a Stereotaxic Device

Fluid-cell Raman Spectroscopy for operando Studies of Reaction and Transport Phenomena during Silicate Glass Corrosion

Quantitative Structure-Activity Relationship, Activity Prediction, and Molecular Dynamics of Non-nucleotide Reverse Transcriptase Inhibitors

High-Resolution C. elegans Imaging Across All Larval Stages

Spatially Compact Arrangement of Larval Zebrafish Sections for Spatial Transcriptomic Analysis

Rapid Depletion of Renal Macrophages using Human CD59/Intermedilysin Cell Ablation Tool

Using Laser Scanning Microscopy to Determine Electromigration in Molybdenum Disilicide

Fabrication of High-Quality Whispering Gallery Mode Microbubble Resonators

Mesocosm-Scale Constructed Wetland Design for Wastewater Treatment

Rapid Detection of Fecal Antigen of Helicobacter pylori Infection Based on Double Antibody Sandwich Detection Technology

Cell-Free Dot Blot as a Practical and Adaptable Immunoassay Platform for the Detection of Antibody Response in Human and Animal Sera

Detection of Helicobacter pylori Infection and Antibiotic Resistance via Stool Quantitative Polymerase Chain Reaction Analysis

Isolation, Fixation and Characterization of Juvenile Gilthead Seabream Head Kidney Leukocytes by Flow Cytometry

Three-dimensional Location Approach with Silk Thread Guided Laparoscopic Segmentectomy for Liver Tumor

Preventing Hemorrhage After Endoscopic Submucosal Dissection Using Polysaccharide Hemostatic Materials

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation

Novel Triple-Loop Technique for Suturing TFCC Injuries without Transosseous Tunnel

Laparoscopic-Assisted Seldinger Technique for Peritoneal Dialysis Catheter Insertion

Mechanism of Shu-Mu Brain-Kidney Acupoint in Treating Oligoasthenospermia in Rats via the Hypothalamic-Pituitary-Testicular Axis

Using a 1064-nm Picosecond Neodymium-Doped Yttrium Aluminum Garnet Laser for Periorbital Hyperpigmentation

Vascularized Composite Hand Allograft Procurement and Preparation for Distal and Proximal Forearm Allotransplantation: A Stepwise Approach

Establishment and Validation of a Rat Model of Pulmonary Arterial Hypertension Associated with Pulmonary Fibrosis

Laparoscopic Anatomical Liver Segment VII Resection with Liver Parenchymal Transection Following a Priority Approach

Endoscopic Approach for Colloid Cyst Resection

Reinforcement Reconstruction using Fascia Lata Autograft for Rhomboid Paralysis-Induced Scapular Winging

Murine Model of Thoracic Aortic Dissection Induced by Oral β-Aminopropionitrile and Subcutaneous Angiotensin II Infusion

Demystifying Venous Excess Ultrasound (VExUS): Image Acquisition and Interpretation

Chinese Herbal Retention Enema for the Treatment of Ulcerative Colitis

Studying Copper Nanoparticle-Induced Programmed Cell Death in Bacteria

Dermoscopy Aids in the Diagnosis of Discoid Lupus Erythematosus

Preliminary Study on Acupuncture Combined with Grain-sized Moxibustion for Treating Rheumatoid Arthritis with Finger Joint Pain

Evaluation of Lung Function in an Ex vivo Murine Model

Three-Dimensional Reconstruction of Orbital Fractures

A Rehabilitation Program of Exoskeleton-assisted Body Weight-Supported Treadmill Training with Non-immersive Virtual Reality for Stroke Patients

Anterior Capsular Reconstruction with Human Dermal Allograft for Irreparable Subscapularis Tears

Isolation of Primary Human Proximal Tubule Epithelial Cells and Their Use in Creating a Microphysiological Model of the Renal Proximal Tubule

Laparoscopic S7 Hepatectomy with Positive Fluorescence Staining

Mouse Model of Coronary Collateral Growth Through Repetitive Ischemia

Dermal Allograft and Biceps Superior Capsule Reconstruction for Massive Irreparable Rotator Cuff Tears

Kidney Procurement in a Preclinical Large Animal Model

Laparoscopic Anatomical Resection of The Right Anterior Lobe Based on The Laennec Capsule Technique

Laparoscopic Common Bile Duct Exploration Followed by Primary Suture Using a Modified Bile Duct Incision

Fluorescence-Guided Matrix-assisted Laser Desorption/Ionization with Laser-Induced Postionization Mass Spectrometry of Individual Rat Neural Cells