AB بامتنان المؤسسة الوطنية للعلوم لتمويل في إطار برنامج سايبر سيستمز الفيزيائية (1239243) وشعبة الجامعية التعليم (1245680)؛ وكالة البحوث المتقدمة الدفاع المشروع (DARPA) لدعم المراحل السابقة من هذا العمل. تم تنفيذ المراحل السابقة من هذا العمل في مختبر البروفيسور AB أميت لال في جامعة كورنيل. AB بفضل Ayesa سينها والبروفيسور لال لتوجيهات التجريبية وتوليد الفكرة في تلك المرحلة. ماندوكا سيكستا (لينيوس 1763) تم الحصول عليها من مستعمرة يحتفظ بها قسم الأحياء في جامعة ديوك، دورهام، كارولينا الشمالية، الولايات المتحدة الأمريكية. واستخدمت العث في غضون 5 أيام من eclosion. نود أن نشكر المثلث النظم البيولوجية الدولية، وخاصة ديفيد Juranas وكاتي ميلاي للحصول على مساعدة فنية ممتازة واستخدام نظام Neuroware بهم. نحن أيضا نود أن نشكر ويل كافي لمساعدته خلال التجارب.

<ol>
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L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Remodeling+of+the+insect+nervous+system.">Remodeling of the insect nervous system.</a> Current opinion in neurobiology. 5 (1), 28-35 (1995).</li><li>Williams, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Physiology+of+insect+diapause:+the+role+of+the+brain+in+the+production+and+termination+of+pupal+dormancy+in+the+giant+silkworm+Platysamia+cecropia.">Physiology of insect diapause: the role of the brain in the production and termination of pupal dormancy in the giant silkworm Platysamia cecropia.</a> Bio. Bull. 90, 234-243 (1946).</li><li>Williams, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+juvenile+hormone.+II.+Its+role+in+the+endocrine+control+of+molting,+pupation,+and+adult+development+in+the+Cecropia+silkworm.">The juvenile hormone. II. Its role in the endocrine control of molting, pupation, and adult development in the Cecropia silkworm.</a> Bio. Bull. 121, 572-585 (1961).</li><li>Bozkurt, A., Lal, A., Gilmour, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Radio+control+of+insects+for+biobotic+domestication.">Radio control of insects for biobotic domestication.</a> 4th International IEEE/EMBS Conference on Neural Engineering. , 215-218 (2009).</li><li>Bozkurt, A., Gilmour, R. F., Lal, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+electrochemical+characterization+of+a+tissue&#8211;electrode+interface+during+metamorphic+growth.">In vivo electrochemical characterization of a tissue&#8211;electrode interface during metamorphic growth.</a> IEEE Transactions on Biomedical Engineering. 58 (8), 2401-2406 (2011).</li><li>Bozkurt, A., Gilmour, R. F., Lal, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Insect&#8211;machine+interface+based+neurocybernetics.">Insect&#8211;machine interface based neurocybernetics.</a> IEEE Transactions on Biomedical Engineering. 56 (6), 1727-1733 (2009).</li><li>Chapman, R. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> The Insects: Structure and Function. , (1998).</li><li>Eaton, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Morphology+of+the+head+and+thorax+of+the+adult+tobacco+hornworm,+Manduca+sexta+(Lepidoptera:Sphingidae).+I.+Skeleton+and+muscles.">Morphology of the head and thorax of the adult tobacco hornworm, Manduca sexta (Lepidoptera:Sphingidae). I. Skeleton and muscles.</a> Annals of the Entomological Society of America. 64, 437-445 (1971).</li><li>Resier, M. B., Dickinson, M. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+modular+display+system+for+insect+behavioral+neuroscience.">A modular display system for insect behavioral neuroscience.</a> Journal of Neuroscience Methods. 167 (2), 127-139 (2008).</li><li>Dombeck, D. A., Reiser, M. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Real+neuroscience+in+virtual+worlds.">Real neuroscience in virtual worlds.</a> Current opinion in neurobiology. 22 (1), 3-10 (2011).</li><li>Weir, P. T., Dickinson, M. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Flying+drosophila+orient+to+sky+polarization.">Flying drosophila orient to sky polarization.</a> Current Biology. 22 (1), 21-27 (2012).</li><li>Ristroph, L., Bergou, A. 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G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Processing+of+artificial+visual+feedback+in+the+walking+fruit+fly+Drosophila+melanogaster.">Processing of artificial visual feedback in the walking fruit fly Drosophila melanogaster.</a> The Journal of experimental biology. 20 (9), 1281-1296 (1997).</li><li>Lindemann, J., Kern, R., Michaelis, C., Meyer, P., van Hateren, J., Egelhaaf, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=FliMax,+a+novel+stimulus+device+for+panoramic+and+highspeed+presentation+of+behaviourally+generated+optic+flow.">FliMax, a novel stimulus device for panoramic and highspeed presentation of behaviourally generated optic flow.</a> Vision Research. 43 (7), 779-791 (2003).</li><li>Reiser, M. B., Dickinson, M. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+modular+display+system+for+insect+behavioral+neuroscience.">A modular display system for insect behavioral neuroscience.</a> Journal of neuroscience methods. 167 (2), 127-139 (2008).</li><li>Bozkurt, A., Gilmour, R. F., Lal, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Balloon-assisted+flight+of+radio-controlled+insect+biobots.">Balloon-assisted flight of radio-controlled insect biobots.</a> IEEE Transactions on Biomedical Engineering. 56 (9), 2304-2307 (2009).</li></ol>

Authors have no conflict of interest in this study.

وهناك العديد من الخطوات الحاسمة خلال الإدراج الجراحي للأقطاب تسجيل التي تؤثر على القدرة على تسجيل البيانات في خطوات لاحقة من البروتوكول. ينبغي إدراج الأقطاب تسجيل في خادرة بعد يوم واحد واظهار البقع الجناح على الجانب الظهري لها. إذا أجري الإدراج بعد يومين أو أكثر هذه...

إدراج الأقطاب، حتى مع تعلق الأنظمة الإلكترونية للحشرات لتطبيقات القياس عن بعد وقد تم تسجيل، وهي طريقة رئيسية لفهم كيفية عمل النظم العصبية أثناء الطيران الطبيعية 1. ربط أو زرع أنظمة اصطناعية في الحشرات قد طرحت العديد من التحديات التي تنطوي على إمكانية زعزعة الرحلة الطبيعية للحشرة. مرفق سطحية أو الإدراج الجراحية للمنصات الاصطناعي على الحشرة الكاملة لا يمكن الاعتماد عليها بسبب التحول ممكن من الأجهزة إدراج الناجمة عن الإجهاد بالقصور الذاتي والقوات التي يسببها الجسم. تعلق بشكل سطحي أو أقطاب إدراج جراحيا هي أيضا عرضة للرفض من قبل الحشرات كهيئة الأجنبية. علاوة على ذلك، يتطلب عملية زرع إزالة القشور وأكوام حول الهيكل الخارجي. يحتاج طبقة بشرة سميكة أيضا أن توغلت لinnervations الجراحية التي يمكن أن تسبب تلف الأنسجة ضمانات، وبالتالي التدخل في الرحلة الطبيعية للحشرة. كل رويمكن لعوامل ذات المناظر جعل عملية الزرع الجراحية أو سطحية مهمة صعبة وحساسة. من أجل التخفيف من حدة هذه المخاوف المشاركة في ربط خارجيا أنظمة التحكم والاستشعار للحشرات، ويمكن وصفها منهجية الرواية التي تنطوي على نمو المتحولة في هذه المقالة. تطوير المتحولة من الحشرات holometabolic يبدأ مع تحول اليرقة (أو حورية) إلى الكبار مع مرحلة العذراء وسيطة (الشكل 1). عملية التحول ينطوي على إعادة برمجة الأنسجة واسعة النطاق بما في ذلك انحطاط تليها إعادة عرض. هذا التحول يتحول إلى يرقة الأرضية الكبار الحشرات يتظاهرون العديد من السلوكيات المعقدة 2،3. وقد تجلى بقاء الحشرات بعد العمليات الجراحية التعايش الالتصاقي القصوى حيث أجريت العمليات الجراحية خلال المراحل المبكرة 4،5 المتحولة. في هذه العمليات الجراحية، ومركز دراسات الوحدة العربية تكون الأنسجة التنمويةالجروح الجراحية إد إلى إصلاح في فترات أقصر. بعد هذه الملاحظات، وقد تم تطوير تقنية جديدة حيث تم إجراء زرع الأقطاب الكهربائية الموصلة كهربائيا خلال المراحل السابقة من النمو المتحولة (الشكل 1). وهذا يتيح مرفق آمن biomechanically على الحشرات 6. يتم تأمين واجهة موثوق بها للغاية أيضا مع العصبية للحشرة والأنظمة العصبية والعضلية 7. ويعرف هذا الأسلوب باسم &quot;التحول المبكر الإدراج التكنولوجيا&quot; (تنبعث) 8. بعد إعادة بناء نظام كامل الأنسجة والهياكل إدراجها في الظهور مع خادرة الحشرة الكبار. المجموعات العضلية طيران ما يصل إلى 65٪ من مجموع كتلة الجسم الصدرية، وبالتالي هو هدف مريحة نسبيا لإجراء تنبعث 9. خلال ضربات الجناح الأساسية، والتغييرات في مورفولوجية الرحلة المحرك dorsolongitudinal (دل) وظهري بطني (DV) العضلات يسبب articulat الجناحأيون الهندسة لتوليد رفع 10. ولذا كان التنسيق الوظيفي من دل والعنف المنزلي العضلات على موضوع البحث النشطة في إطار الفسيولوجيا العصبية الطيران. وقد تم الربط الحشرات في بيئات بصرية مبرمجة إلكترونيا الأسلوب الأكثر شيوعا لدراسة الفسيولوجيا العصبية من السلوكيات المعقدة الحركي 11،12. وقد استخدمت الساحات أسطواني يتكون من لوحات الصمام الثنائي الباعثة للضوء لهذه البيئات الواقع الافتراضي، حيث يتم المربوطة الحشرات الطائرة في وسط ومحاكاة الحركة عن طريق تحديث حيوي العرض المرئي بانورامية المحيطة بها. في حالة الحشرات الأصغر حجما، مثل ذبابة الفاكهة ذبابة الفاكهة، ويتحقق الربط عن طريق ربط مسمار معدني إلى القفص الصدري الظهري من الحشرات ووضع دبوس تحت المغناطيس الدائم 13،14. هذا الأسلوب يسمح فقط الكمي من الاستجابات الحركية من خلال الملاحظات البصرية مع كاميرات عالية السرعة دون أي تحليل الكهربية. وعلاوة على ذلك، وهذا ميثوقد التطوير التنظيمي غير فعالة لوقف الجسم أكبر وأثقل من ماندوكا سيكستا. لحل هذه المشكلة، ونحن استفادت من إطارات الرفع مغناطيسيا حيث إطارات خفيفة الوزن مع ومرفوع مغناطيس تعلق على الجزء السفلي من خلال القوى الكهرومغناطيسية. عند دمجها مع مكبرات الصوت المتاحة تجاريا العصبية والمصفوفات LED، وهذا يوفر منبرا للسيطرة على الانتاج طيران الحركية وتسجيل الكهربية ذات الصلة ماندوكا سيكستا.

<table border="0" cellpadding="0" cellspacing="0" width="579">
	<tbody>
		<tr height="63">
			<td height="63" style="height:63px;width:195px;">Coated stainless steel wire</td>
			<td style="width:113px;">A-M Systems</td>
			<td style="width:137px;">791900</td>
			<td style="width:135px;">0.008&#8217;&#8217; bare, 0.011&#8217;&#8217; coated, annealed</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:195px;">Flexible electrode wire</td>
			<td style="width:113px;"></td>
			<td style="width:137px;"></td>
			<td style="width:135px;">Litz or inductor wire can be used.&#160;</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:195px;">Surface-mount FFC connector</td>
			<td style="width:113px;">Hirose Connector</td>
			<td style="width:137px;">FH28E-20S-0.5SH(05)</td>
			<td style="width:135px;"></td>
		</tr>
		<tr height="63">
			<td height="63" style="height:63px;width:195px;">Tweezers</td>
			<td style="width:113px;">Grobet USA</td>
			<td style="width:137px;">N/A</td>
			<td style="width:135px;">Clean with 70% alcohol before use on the insect.</td>
		</tr>
		<tr height="63">
			<td height="63" style="height:63px;width:195px;">Kim-Wipes</td>
			<td style="width:113px;">Kimberly-Clark Worldwide</td>
			<td style="width:137px;">34155</td>
			<td style="width:135px;">Any size delicate-wipe tissues can be used.</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:195px;">Teflon tape</td>
			<td style="width:113px;">N/A</td>
			<td style="width:137px;">N/A</td>
			<td style="width:135px;">5 mm width Teflon tape.</td>
		</tr>
		<tr height="84">
			<td height="84" style="height:84px;width:195px;">Hypodermic Needle</td>
			<td style="width:113px;">Becton Dickinson &#38; Co.</td>
			<td style="width:137px;">30511</td>
			<td style="width:135px;">20-30 gauge hypodermic needle can be used. Video showed 30 gauge.</td>
		</tr>
		<tr height="84">
			<td height="84" style="height:84px;width:195px;">Rigid Fixation Stick</td>
			<td style="width:113px;">N/A</td>
			<td style="width:137px;">N/A</td>
			<td style="width:135px;">Variety of materials can be used (e.g. coffee stirrers)</td>
		</tr>
		<tr height="126">
			<td height="126" style="height:126px;width:195px;">Insect Emergence Cage</td>
			<td style="width:113px;">N/A</td>
			<td style="width:137px;">N/A</td>
			<td style="width:135px;">Plastic pet cage lined with packing paper or similar padding. Ventilation holes are needed.</td>
		</tr>
		<tr height="84">
			<td height="84" style="height:84px;width:195px;">Thermal Cauterizer</td>
			<td style="width:113px;">Advanced Meditech International</td>
			<td style="width:137px;">CH-HI CT2103 (tip)</td>
			<td style="width:135px;">Optional equipment used for application of dental wax.</td>
		</tr>
		<tr height="84">
			<td height="84" style="height:84px;width:195px;">Dental Wax</td>
			<td style="width:113px;">Orthomechanics LC., Broken Arrow, Oklahoma</td>
			<td style="width:137px;">N/A</td>
			<td style="width:135px;">Optional material used for stabilizing the electrodes on the insect.</td>
		</tr>
		<tr height="80">
			<td height="80" style="height:80px;width:195px;">Magnetic Levitation Platform</td>
			<td style="width:113px;">N/A</td>
			<td style="width:137px;">N/A</td>
			<td style="width:135px;">Custom designed frame fabricated in-house with 3D prototyping</td>
		</tr>
		<tr height="80">
			<td height="80" style="height:80px;width:195px;">CA40 Instant Adhesive</td>
			<td style="width:113px;">3M</td>
			<td style="width:137px;">62-3803-0330-5</td>
			<td style="width:135px;">Avoid skin contact. Use gloves when handling.</td>
		</tr>
		<tr height="60">
			<td height="60" style="height:60px;width:195px;">70% Isopropyl alcohol</td>
			<td style="width:113px;">store brand</td>
			<td style="width:137px;"></td>
			<td style="width:135px;">Commercially available from many suppliers.</td>
		</tr>
		<tr height="80">
			<td height="80" style="height:80px;width:195px;">PCB Etchant</td>
			<td style="width:113px;">RadioShack</td>
			<td style="width:137px;">276-1535</td>
			<td style="width:135px;">Toxic if swallowed or ingested, skin irritant&#160;</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:195px;"></td>
			<td style="width:113px;"></td>
			<td style="width:137px;"></td>
			<td style="width:135px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">EQUIPMENT:</td>
			<td style="width:113px;"></td>
			<td style="width:137px;"></td>
			<td style="width:135px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:195px;">Name of Reagent/Material</td>
			<td style="width:113px;">Company</td>
			<td style="width:137px;">Catalog Number</td>
			<td>Comments</td>
		</tr>
		<tr height="231">
			<td height="231" style="height:231px;width:195px;">TBSI Neuroware Software</td>
			<td style="width:113px;">Triangle Biosystems International</td>
			<td style="width:137px;">N/A</td>
			<td style="width:135px;">NeuroWare v1.4. Software for importing .nex files obtained at Technologies, N. NeuroExplorer Code and Scripts. (2012).at http://www.neuroexplorer.com/code.html</td>
		</tr>
		<tr height="63">
			<td height="63" style="height:63px;width:195px;">TBSI Wireless Recording System</td>
			<td style="width:113px;">Triangle Biosystems International</td>
			<td style="width:137px;">W5 FI</td>
			<td style="width:135px;">USB Base station, headstage unit, charger</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:195px;">16 Channel Amplifier A-C Amplifier</td>
			<td style="width:113px;">A-M Systems&#160;</td>
			<td style="width:137px;">950000</td>
			<td style="width:135px;">Model 3500 (110 V)</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:195px;">Oscilloscope&#160;</td>
			<td style="width:113px;">Agilent Technologies&#160;</td>
			<td style="width:137px;">DSO1014A</td>
			<td style="width:135px;">Oscilloscope, 100 MHz, 4 channel</td>
		</tr>
		<tr height="120">
			<td height="120" style="height:120px;width:195px;">Microscope</td>
			<td style="width:113px;">N/A</td>
			<td style="width:137px;">N/A</td>
			<td style="width:135px;">5x magnification microscope to assist visualization during electronics construction.&#160;</td>
		</tr>
		<tr height="60">
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early metamorphic insertion technology for insect flight behavior monitoring

Early Metamorphosis Insertion Technology (EMIT) is a novel methodology for integrating microfabricated neuromuscular recording and actuation platforms on insects during their metamorphic development. Here, the implants are fused within the structure and function of the neuromuscular system as a result of metamorphic tissue remaking. The implants emerge with the insect where the development of tissue around the electronics during pupal development results in a bioelectrically and biomechanically enhanced tissue interface. This relatively more reliable and stable interface would be beneficial for many researchers exploring the neural basis of the insect locomotion with alleviated traumatic effects caused during adult stage insertions. In this article, we implant our electrodes into the indirect flight muscles of Manduca sexta. Located in the dorsal-thorax, these main flight powering dorsoventral and dorsolongitudinal muscles actuate the wings and supply the mechanical power for up and down strokes. Relative contraction of these two muscle groups has been under investigation to explore how the yaw maneuver is neurophysiologically coordinated. To characterize the flight dynamics, insects are often tethered with wires and their flight is recorded with digital cameras. We also developed a novel way to tether Manduca sexta on a magnetically levitating frame where the insect is connected to a commercially available wireless neural amplifier. This set up can be used to limit the degree of freedom to yawing &#8220;only&#8221; while transmitting the related electromyography signals from dorsoventral and dorsolongitudinal muscle groups.

ويرد التخطيطي الإجراء تنبعث الشاملة في الشكل 1، والتي تبين المراحل الرئيسية في دورة المتحولة في hawkmoth والخطوات القطب الإدراج المقابلة. يجب أن يتم تنفيذ الإدراج القطب في مرحلة العذراء في وقت متأخر قبل 4-7 أيام eclosion. وهذا يسمح للألياف العضلات في جميع أنحاء لتطوي...

Watch this Scientific Journal Video about Early Metamorphic Insertion Technology for Insect Flight Behavior Monitoring at JoVE.com

Early Metamorphic Insertion Technology for Insect Flight Behavior Monitoring

We present a novel surgical procedure to implant electrodes in Manduca sexta during its early metamorphic stages. This technique allows mechanically stable and electrically reliable coupling with the neuromuscular tissue to study flight neurophysiology dynamics. We also present a novel magnetic levitation platform for tethered studies of insect yaw.

المتحولة الإدراج المبكر لتكنولوجيا الطيران سلوك الحشرات الرصد

Early Metamorphosis Insertion Technology (EMIT) is a novel methodology for integrating microfabricated neuromuscular recording and actuation platforms on ...

early-metamorphic-insertion-technology-for-insect-flight-behavior

Research

JoVE Journal

Behavior

14.4K Views.  North Carolina State University. We present a novel surgical procedure to implant electrodes in Manduca sexta during its early metamorphic stages. This technique allows mechanically stable and electrically reliable coupling with the neuromuscular tissue to study flight neurophysiology dynamics. We also present a novel magnetic levitation platform for tethered studies of insect yaw.

Video: Early Metamorphic Insertion Technology for Insect Flight Behavior Monitoring

Examining Recall Memory in Infancy and Early Childhood Using the Elicited Imitation Paradigm

The ability to recall the past allows us to report on details of previous experiences, from the everyday to the significant. Because recall memory is commonly assessed using verbal report paradigms in adults, studying the development of this ability in preverbal infants and children proved challenging. Over the past 30 years, researchers have developed a non-verbal means of assessing recall memory known as the elicited or deferred imitation paradigm. In one variant of the procedure, participants are presented with novel three-dimensional stimuli for a brief baseline period before a researcher demonstrates a series of actions that culminate in an end- or goal-state. The participant is allowed to imitate the demonstrated actions immediately, after a delay, or both. Recall performance is then compared to baseline or to performance on novel control sequences presented at the same session; memory can be assessed for the individual target actions and the order in which they were completed. This procedure is an accepted analogue to the verbal report techniques used with adults, and it has served to establish a solid foundation of the nature of recall memory in infancy and early childhood. In addition, the elicited or deferred imitation procedure has been modified and adapted to answer questions relevant to other aspects of cognitive functioning. The broad utility and application of imitation paradigms is discussed, along with limitations of the approach and directions for future research.

The elicited imitation procedure was established to examine the development of recall memory in infancy and early childhood. This procedure has been widely used to establish a solid foundation of the nature of recall memory in infancy and early childhood.

دراسة استدعاء الذاكرة في مرحلة الرضاعة والطفولة المبكرة باستخدام أثارت تقليد النموذج

The ability to recall the past allows us to report on details of previous experiences, from the everyday to the significant. Because recall memory is ...

Protocol for Data Collection and Analysis Applied to Automated Facial Expression Analysis Technology and Temporal Analysis for Sensory Evaluation

We demonstrate a method for capturing emotional response to beverages and liquefied foods in a sensory evaluation laboratory using automated facial expression analysis (AFEA) software. Additionally, we demonstrate a method for extracting relevant emotional data output and plotting the emotional response of a population over a specified time frame. By time pairing each participant's treatment response to a control stimulus (baseline), the overall emotional response over time and across multiple participants can be quantified. AFEA is a prospective analytical tool for assessing unbiased response to food and beverages. At present, most research has mainly focused on beverages. Methodologies and analyses have not yet been standardized for the application of AFEA to beverages and foods; however, a consistent standard methodology is needed. Optimizing video capture procedures and resulting video quality aids in a successful collection of emotional response to foods. Furthermore, the methodology of data analysis is novel for extracting the pertinent data relevant to the emotional response. The combinations of video capture optimization and data analysis will aid in standardizing the protocol for automated facial expression analysis and interpretation of emotional response data.

A protocol for capturing and statistically analyzing emotional response of a population to beverages and liquefied foods in a sensory evaluation laboratory using automated facial expression analysis software is described.

بروتوكول لجمع البيانات وتحليل التطبيقية لالآلي الوجه تقنية تحليل التعبير والتحليل الزمني للتقييم الحسي

We demonstrate a method for capturing emotional response to beverages and liquefied foods in a sensory evaluation laboratory using automated facial ...

An Objective and Child-friendly Assessment of Arm Function by Using a 3-D Sensor

Progressive and irreversible muscle atrophy characterizes Spinal Muscular Atrophy (SMA) and other similar muscle disorder diseases. Objective assessment of muscle functions is an essential and important, although challenging, prerequisite for successful clinical trials. Current clinical rating scales restrain the movement abnormalities to certain predefined coarse-grained individual items. The Kinect 3-D sensor has emerged as a low-cost and portable motion sensing technology used to capture and track people&#39;s movement in many medical and research fields. A novel approach using this 3-D sensor was developed and a game-like test was designed to objectively measure the upper limb function of patients with SMA. The prototype test targeted joint movement capability. While sitting in a virtual scene, the patient was instructed to extend, flex, and lift the whole arm in order to reach and place some objects. Both kinematic and spatiotemporal characteristics of upper limb movement were extracted and analyzed, e.g., elbow extension and flexion angles, hand velocity, and acceleration. The first study included a small cohort of 18 ambulant SMA patients and 19 age- and gender-matched healthy controls. A comprehensive analysis of arm movement was achieved; however, no significant difference between the groups were found due to the mismatch of patient&#39;s capability and the test difficulty. Based on this experience, a second version of the test consisting of a modified version of the first game with increased difficulties and a second game targeting muscle endurance were designed and implemented. The new test has not been conducted in any patient groups yet. Our work has demonstrated the potential capability of the 3-D sensor in assessing such muscle function and suggested an objective approach to complement the clinical rating scales.

An objective measure of muscle functions is challenging especially in children. Based on a commercially available digital 3-D sensor, a child-friendly gaming test was developed to assess upper limb function for clinical trials.

تقييم موضوعي والصديقة للطفل لوظيفة الذراع باستخدام جهاز استشعار ثلاثي الأبعاد

Progressive and irreversible muscle atrophy characterizes Spinal Muscular Atrophy (SMA) and other similar muscle disorder diseases. Objective assessment ...

Key Elements of Photo Attraction Bioassay for Insect Studies or Monitoring Programs

Optimized visual attractants will increase insect trapping efficiency by using the target insect&#39;s innate behaviors (positive photo-taxis) as a means to lure the insect into a population control or monitoring trap. Light emitting diodes (LEDs) have created customizable lighting options with specific wavelengths (colors), intensities, and bandwidths, all of which can be customized to the target insects. Photo-attraction behavioral bioassays can use LEDs to optimize the attractive color(s) for an insect species down to specific life history stages or behaviors (mating, feeding, or seeking shelter). Researchers must then confirm the bioassay results in the field and understand the limited attractive distance of the visual attractants.
The cloverleaf bioassay arena is a flexible method to assess photo attraction while also assessing a range of natural insect behaviors such as escape and feeding responses. The arena can be used for terrestrial or aerial insect experiments, as well as diurnal, and nocturnal insects. Data collection techniques with the arena are videotaping, counting contact with the lights, or physically collecting the insects as they are attracted towards the lights. The assay accounts for insects that make no-choice and the arenas can be single (noncompetitive) color or multiple (competitive) colors. The cloverleaf design causes insects with strong thigmotaxis to return to the center of the arena where they can view all the options in a competitive LED tests. The cloverleaf arena presented here has been used with mosquitoes, bed bugs, Hessian fly, house flies, biting midges, red flour beetles, and psocids. Bioassays are used to develop accurate and effective insect traps to guide the development and optimization of insect traps used to monitor pest population fluctuations for disease vector risk assessments, the introduction of invasive species, and/or be used for population suppression.

Photo-attraction bioassay arenas are used to determine the optimal light color(s) to maximize insect attraction; however bioassays and methods are specific to target insect behaviors and habitats. Customizable equipment and modifications are explained for nocturnal or diurnal and terrestrial or aerial insects.

العناصر الرئيسية لصور الجذب الحشري لدراسات الحشرات أو رصد البرامج

Optimized visual attractants will increase insect trapping efficiency by using the target insect&#39;s innate behaviors (positive photo-taxis) as a means ...

A Wind Tunnel for Odor Mediated Insect Behavioural Assays

Olfaction is the most important sensory mechanism by which many insects interact with their environment and a wind tunnel is an excellent tool to study insect chemical ecology. Insects can locate point sources in a three-dimensional environment through the sensory interaction and sophisticated behavior. The quantification of this behavior is a key element in the development of new tools for pest control and decision support. A wind tunnel with a suitable flight section with laminar air flow, visual cues for in-flight feedback and a variety of options for the application of odors can be used to measure complex behaviour which subsequently may allow the identification of attractive or repellent odors, insect flight characteristics, visual-odor interactions and interactions between attractants and odors lingering as background odors in the environment. A wind tunnel holds the advantage of studying the odor mediated behavioural repertoire of an insect in a laboratory setting. Behavioural measures in a controlled setting provide the link between the insect physiology and field application. A wind tunnel must be a flexible tool and should easily support the changes to setup and hardware to fit different research questions. The major disadvantage to the wind tunnel setup described here, is the clean odor background which necessitates special attention when developing a synthetic volatile blend for field application.

Here, we describe the construction and use of a wind tunnel for odor mediated behavioural assays with insects. The wind tunnel design facilitates the release of odor sources by several methods, with and without visual stimuli. Wind tunnel experiments are important methods to identify behaviorally active volatile chemicals.

نفق الرياح لرائحة بوساطة الحشرات فحوصات السلوكية

Olfaction is the most important sensory mechanism by which many insects interact with their environment and a wind tunnel is an excellent tool to study ...

Using Flight Mills to Measure Flight Propensity and Performance of Western Corn Rootworm, Diabrotica virgifera virgifera (LeConte)

The western corn rootworm, Diabrotica virgifera virgifera (LeConte) (Coleoptera: Chrysomelidae), is an economically important pest of corn in the northern United States. Some populations have developed resistance to management strategies including transgenic corn that produces insecticidal toxins derived from the bacterium Bacillus thuringiensis (Bt). Knowledge of western corn rootworm dispersal is of critical importance for models of resistance evolution, spread, and mitigation. Flight behavior of an insect, especially over a long distance, is inherently difficult to observe and characterize. Flight mills provide a means to directly test developmental and physiological impacts and consequences of flight in the laboratory that cannot be obtained in field studies. In this study, flight mills were used to measure the timing of flight activity, total number of flights, and the distance, duration, and speed of flights taken by female rootworms during a 22-h test period. Sixteen flight mills were housed in an environmental chamber with programmable lighting, temperature, and humidity control. The flight mill described is of a typical design, where a flight arm is free to rotate about a central pivot. Rotation is caused by flight of an insect tethered to one end of the flight arm, and each rotation is recorded by a sensor with a time-stamp. Raw data are compiled by software, which are subsequently processed to provide summary statistics for flight parameters of interest. The most difficult task for any flight mill study is attachment of the tether to the insect with an adhesive, and the method used must be tailored to each species. The attachment must be strong enough to hold the insect in a rigid orientation and to prevent detachment during movement, while not interfering with natural wing motion during flight. The attachment process requires dexterity, finesse, and speed, making video footage of the process for rootworms of value.

Using Flight Mills to Measure Flight Propensity and Performance of Western Corn Rootworm, Diabrotica virgifera virgifera LeConte

Flight mills are important tools for comparing how age, sex, mating status, temperature, or various other factors may influence an insect&#8217;s flight behavior. Here we describe protocols to tether and measure the flight propensity and performance of western corn rootworm under different treatments.

باستخدام مطاحن الطيران لقياس الميل الطيران والأداء من الجذور الخفية الذرة الغربية ، ديروتيكا virgifera virgifera (leconte)

The western corn rootworm, Diabrotica virgifera virgifera (LeConte) (Coleoptera: Chrysomelidae), is an economically important pest of corn in the northern ...

Nest Building Behavior as an Early Indicator of Behavioral Deficits in Mice

Nest building is an innate behavior in male and female rodents, even when raised in laboratory settings. As such, many researchers provide rodents synthetic and/or natural materials (such as twine, tissue, cotton, paper, and hay) as a gauge of their overall well-being and as an ancillary assessment to predict the possible decline in cognition. Typically, changes in nesting behaviors, such as failure to create a nest, indicate a change in health or welfare. In addition, nesting behavior is sensitive to many environmental and physiological challenges, as well as many genetic mutations underlying pathological disease states. The following protocol describes a nesting behavior paradigm that explores the usage of four types of nesting material. In addition, the protocol utilizes intraclass correlations to demonstrate that inter-rater reliability is higher when nests are constructed out of shredded paper compared to other common nesting materials such as cotton squares, paper twists, and soft cob bedding. The chosen methodology and statistical considerations (i.e., intraclass correlation) for this assay may be of interest for those conducting experiments assessing the quality of living of mice.&#160;

Here, we present a protocol to quantify nest building behavior in mice, which is known to be impaired in several neurological disorders and diseases. This protocol examines the utility of four materials and offers the opportunity to quantify the rater agreement in scoring, improving the validity and reliability of the assay.

سلوك بناء العش كمؤشر مبكر للعجز السلوكي في الفئران

Nest building is an innate behavior in male and female rodents, even when raised in laboratory settings. As such, many researchers provide rodents ...

A Novel Pavlovian Fear Conditioning Paradigm to Study Freezing and Flight Behavior

Fear- and anxiety-related behaviors significantly contribute to an organism&#8217;s survival. However, exaggerated defensive responses to perceived threat are characteristic of various anxiety disorders, which are the most prevalent form of mental illness in the United States. Discovering the neurobiological mechanisms responsible for defensive behaviors will aid in the development of novel therapeutic interventions. Pavlovian fear conditioning is a widely used laboratory paradigm to study fear-related learning and memory. A major limitation of traditional Pavlovian fear conditioning paradigms is that freezing is the only defensive behavior monitored. We recently developed a modified Pavlovian fear conditioning paradigm that allows us to study both conditioned freezing and flight (also known as escape) behavior within individual subjects. This model employs higher intensity footshocks and a greater number of pairings between the conditioned stimulus and unconditioned stimulus. Additionally, this conditioned flight paradigm utilizes serial presentation of pure tone and white noise auditory stimuli as the conditioned stimulus. Following conditioning in this paradigm, mice exhibit freezing behavior in response to the tone stimulus, and flight responses during the white noise. This conditioning model can be applied to the study of rapid and flexible transitions between behavioral responses necessary for survival.

Defensive behavioral responses are contingent upon threat intensity, proximity, and context of exposure. Based on these factors, we developed a classical conditioning paradigm that elicits clear transitions between conditioned freezing and flight behavior within individual subjects. This model is crucial for the understanding the pathologies involved in anxiety, panic, and post-traumatic stress disorders.

رواية بافلوفيان الخوف تكييف نموذج لدراسة تجميد وسلوك الطيران

Fear- and anxiety-related behaviors significantly contribute to an organism&#8217;s survival. However, exaggerated defensive responses to perceived threat ...

The Participant-Reported Implementation Update and Score (PRIUS): A Novel Method for Capturing Implementation-Related Data Over Time

"Implementation" of new initiatives in healthcare settings typically encompasses two distinct components: a "clinical intervention" plus accompanying "implementation strategies" that support putting the clinical intervention into day-to-day practice. A novel clinical intervention, for example, might consist of a new medication, a new protocol, a new device, or a new program. As clinical interventions are not self-implementing, however, they nearly always require effective implementation strategies in order to succeed. Implementation strategies set out to engage healthcare providers, staff and patients in ways that increase the likelihood of the new initiative being successfully adopted, a process that often involves behavior change and new ways of thinking by participants. One of the challenges in studying implementation is that it can be difficult to collect data about the status and progress of implementation, including participants' own perspectives and experiences concerning implementation to date. This protocol describes a novel method for collecting and analyzing data related to ongoing implementation called the Participant-Reported Implementation Update and Score, or PRIUS. The PRIUS method allows for the efficient and systematic capture of qualitative and quantitative data that can provide a detailed and nuanced account of implementation over time and from multiple viewpoints. This longitudinal method can enable researchers, as well as implementation leaders and organizational stakeholders, to monitor implementation progress more closely, conduct formative evaluation, identify improvement opportunities, and gauge the effect of any implementation changes on a rolling basis.

The Participant-Reported Implementation Update and Score PRIUS: A Novel Method for Capturing Implementation-Related Data Over Time

This protocol describes a novel method for collecting and analyzing data related to ongoing implementation called the Participant-Reported Implementation Update and Score (PRIUS). The PRIUS method allows for the efficient and systematic capture of data over time and from multiple viewpoints in healthcare settings.

تحديث التنفيذ والنتيجة التي أبلغ عنها المشاركون (PRIUS): طريقة جديدة لالتقاط البيانات المتعلقة بالتنفيذ بمرور الوقت

"Implementation" of new initiatives in healthcare settings typically encompasses two distinct components: a "clinical intervention" plus accompanying ...

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera (H&#252;bner)

The cotton bollworm, Helicoverpa armigera, is one of the most destructive pests in the world. A combination of molecular genetics, physiology, functional genomics, and behavioral studies has made H. armigera a model species in Lepidoptera Noctuidae. To study the in vivo functions of and interactions between different genes, clustered regularly interspaced short palindromic repeats (CRISPR)/ associated protein 9 (Cas9) genome editing technology is a convenient and effective method used for performing functional genomic studies. In this study, we provide a step-by-step systematic method to complete gene knockout in H. armigera using the CRISPR/Cas9 system. The design and synthesis of guide RNA (gRNA) are described in detail. Then, the subsequent steps consisting of gene-specific primer design for guide RNA (gRNA) creation, embryo collection, microinjection, insect rearing, and mutant detection are summarized. Finally, troubleshooting advice and notes are provided to improve the efficiency of gene editing. Our method will serve as a reference for the application of CRISPR/Cas9 genome editing in H. armigera as well as other Lepidopteran moths.

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera H&amp;#252;bner

Presented here is a protocol of Helicoverpa armigera (H&#252;bner) embryo microinjection and knockout mutant identification created by CRISPR/Cas9 genome editing. Mutant insects enable further research of gene function and interaction among different genes in vivo.

الحقن المجهري للأجنة وتحديد المتحورات بالضربة القاضية ل CRISPR / Cas9 Genome edited Helicoverpa Armigera (Hübner)

The cotton bollworm, Helicoverpa armigera, is one of the most destructive pests in the world. A combination of molecular genetics, physiology, functional ...