وأيد هذا العمل من المنح المقدمة من المعهد القومي للقلب والرئة (R01 HL086582) وصندوق العلوم الخلايا الجذعية نيويورك (NYSTEM، عقد # &#160; C024316) إلى STA وDDS الرسوم التوضيحية المستخدمة في إن الرب فيديو تم الانتهاء من جون نيكويست. المصور الطبية من جامعة ولاية نيويورك في بوفالو.

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والغرض من هذا التقرير هو توفير إجراءات وموثوقة وقابلة للتكرار لTEVs زرع الاهتمام في الشريان السباتي الأغنام. وكانت الشرايين السباتية الأم من الحيوانات المستخدمة في هذا النموذج ،5-0،75 ملم في سمك و4،5-5 ملم وقطرها الخارجي. وكانت تقنية جراحية وصفها هنا ناجحة لزرع TEVs من هندس...

كان التركيز الأساسي لتطوير TEVs لتوفير بديل لاستبدال الكسب غير المشروع ذاتي عندما السفن ذاتي لا تتوفر والحد المانحة البصر الاعتلال. على سبيل المثال، فقد تجاوز عدد العمليات الجراحية الشريان التاجي الالتفافية سنويا 350،000 في الولايات المتحدة الأمريكية، ويبقى مصدرا مثاليا للترقيع الشريان الثديي مناسبة الداخلي الأيسر، الأمامي الأيسر تنازلي الشريان التاجي والوريد الصافن 1. منذ العديد من الأفراد الذين يعانون من أمراض الأوعية الدموية قد لا يكون الشرايين والأوردة المناسبة لاستبدال الكسب غير المشروع ذاتي، وهكذا أصبح تطوير TEVs حقل مكثفة للبحث عن عقود 1-6. في حين الهندسية والاستفادة المثلى من TEVs جديدة خضعت العديد من التطورات، وتقديم التقارير على التقنيات الجراحية المستخدمة لزرع TEVs لم يكن أنفسهم موضوع هذه المناقشة المكثفة. بدلا من ذلك، يتم ترك إلى حد كبير بروتوكولات بشأن زرع TEVs في النماذج الحيوانيةيصل للبحث المحققين. يوضح مخطوطة التالية كيفية زرع TEVs من خلال الاستفادة من نهج مفاغرة نهاية إلى نهاية. وكان هذا الإجراء الأمثل باستخدام نمط معين خياطة تفاغري، وتحقيق الاستقرار تقنية خياطة، وتحسين التوتر الطولي وإضافة في الجسم الحي أجهزة للرصد. ويتناقض هذا الأسلوب مع بعض من كثير من الاختلافات التي تم استخدامها سابقا. وعلاوة على ذلك، يصف هذا الإجراء كيفية اكتساب المعلمات مثل ضغط الدم الشرياني، TEV قطر / الامتثال ومعدل التدفق من خلال TEV بعد الجراحة حتى explantation. يوفر هذا جمع البيانات تحليلا لا غنى عنه في TEV أثناء وجوده في عملية التجديد.

<table border="0" cellpadding="0" cellspacing="0" width="1505">
	<tbody>
		<tr height="43">
			<td dir="LTR" height="43" style="height:43px;width:335px;">Equipment</td>
			<td dir="LTR" style="width:208px;">Manufacturer</td>
			<td dir="LTR" style="width:311px;">Serial/Catalog #</td>
			<td dir="LTR" style="width:363px;">Notes</td>
		</tr>
		<tr height="56">
			<td dir="LTR" height="94" rowspan="2" style="height:94px;width:335px;">Pressure Transducer</td>
			<td dir="LTR" rowspan="2" style="width:208px;">Becton Dickinson</td>
			<td dir="LTR" rowspan="2" style="width:311px;">P23XL-1</td>
			<td dir="LTR" style="width:291px;">1+ (1 for each artery) 
			&#160;Used with water-filled diaphragm domes</td>
		</tr>
		<tr height="172">
			<td dir="LTR" height="172" style="height:172px;width:335px;">Amplifier and transducer box</td>
			<td dir="LTR" style="width:208px;">Gould</td>
			<td dir="LTR" style="width:311px;">5900 Signal Conditioner Cage</td>
			<td dir="LTR" style="width:291px;">1 
			Two transducers and amplifiers should be included in cage. While this specific unit may be discontinued, other commercially available pressure transducers with a BNC/analog output will communicate with the Sonometrics equipment</td>
		</tr>
		<tr height="121">
			<td dir="LTR" height="121" style="height:121px;width:335px;">T403 Console with TS420 perivascular flowmeter module(x2)&#160;</td>
			<td dir="LTR" style="width:208px;">Transonic Systems</td>
			<td dir="LTR" style="width:311px;">T403 module and TS420 (x2)</td>
			<td dir="LTR" style="width:291px;">1 
			Flow probes measuring flow through each of the carotid arteries will connect to each of the TS420 units.&#160;&#160;</td>
		</tr>
		<tr height="126">
			<td dir="LTR" height="126" style="height:126px;width:335px;">Digital ultrasonic measurement unit</td>
			<td dir="LTR" style="width:208px;">Sonometrics</td>
			<td dir="LTR" style="width:311px;">TR-USB</td>
			<td dir="LTR" style="width:291px;">1</td>
		</tr>
		<tr height="48">
			<td dir="LTR" height="48" style="height:48px;width:335px;">Flow Probe Precision S-Series 4mm</td>
			<td dir="LTR" rowspan="4" style="width:208px;">Transonic Systems Inc.</td>
			<td dir="LTR" rowspan="4" style="width:311px;">MC4PSS-LS-WC100-CM4B-GA</td>
			<td dir="LTR" rowspan="4" style="width:291px;">2</td>
			<td dir="LTR" rowspan="4" style="width:363px;"></td>
		</tr>
		<tr height="24">
			<td dir="LTR" height="49" rowspan="2" style="height:49px;width:335px;">1mm&#160; Sonometrics Crystals</td>
			<td dir="LTR" rowspan="2" style="width:208px;">Sonometrics Systems</td>
			<td dir="LTR" rowspan="2" style="width:311px;">1R-38S-20-NC-SH</td>
			<td dir="LTR" style="width:291px;">&#160;2-4 (2 for each artery)</td>
		</tr>
		<tr height="81">
			<td dir="LTR" height="134" rowspan="2" style="height:134px;width:335px;">Catheter for implantation</td>
			<td dir="LTR" style="width:208px;">BD (Becton Dickinson)</td>
			<td dir="LTR" rowspan="2" style="width:311px;">&#160;381447</td>
			<td dir="LTR" style="width:291px;">&#160;1+ (1 for each artery) 
			Catheter is cut and secured to microbore tubing, stylette is utilized for insertion&#160;</td>
		</tr>
		<tr height="24">
			<td dir="LTR" height="73" rowspan="2" style="height:73px;width:335px;">Tygon Microbore Tubing</td>
			<td dir="LTR" rowspan="2" style="width:208px;">Norton Performance Plastics</td>
			<td dir="LTR" style="width:311px;">(AAQ04127) Formulation S-54-HL</td>
			<td dir="LTR" style="width:291px;">NA (cut to length for an extension set)</td>
		</tr>
		<tr height="24">
			<td dir="LTR" height="49" rowspan="2" style="height:49px;width:335px;">Luer Stub Adapter</td>
			<td dir="LTR" style="width:208px;">BD (Becton Dickinson)</td>
			<td dir="LTR" style="width:311px;">427564 (20 gauge)</td>
			<td dir="LTR" style="width:291px;">1+ (1 for each arterial catheter)</td>
		</tr>
		<tr height="24">
			<td dir="LTR" height="82" rowspan="2" style="height:82px;width:335px;">Surflo Injection Plug</td>
			<td dir="LTR" rowspan="2" style="width:208px;">Terumo</td>
			<td dir="LTR" rowspan="2" style="width:311px;">SR-IP2</td>
			<td dir="LTR" style="width:291px;">1+ (1 for each arterial catheter)</td>
		</tr>
		<tr height="39">
			<td dir="LTR" height="128" rowspan="2" style="height:128px;width:335px;">Meadox</td>
			<td dir="LTR" rowspan="2" style="width:208px;">PTFE (Teflon) Felt</td>
			<td dir="LTR" rowspan="2" style="width:311px;">19306</td>
			<td dir="LTR" style="width:291px;">NA (cut to size) 
			The PTFE felt used in our studies was discontinued. However, comparable companies such as &#8220;Surgical Mesh&#8221; offer products which are equivalent.&#160;</td>
		</tr>
	</tbody>
</table>

surgical technique for the implantation of tissue engineered vascular grafts and subsequent in vivo monitoring

وتقدمت تطوير الأنسجة المهندسة سفن (TEVs) من خلال القدرة على TEVs زرع بشكل روتيني وفعال (4-5 ملم في القطر) في نموذج حيواني كبير. يوصف بروتوكول خطوة بخطوة لوضع بين الموضعية للتقييم TEV في الوقت الحقيقي والرقمي للTEV والشرايين السباتية الأم هنا. ويتكون في الجسم الحي الرصد ممكن عن طريق غرس تحقيقات التدفق، والقسطرة وبلورات بالموجات فوق الصوتية (قادرة تسجيل التغييرات قطر الديناميكية للTEVs زرع الشرايين السباتية والأم) في وقت الجراحة. مرة واحدة زرعها، يمكن للباحثين حساب الشرايين أنماط تدفق الدم، وضغط الدم الغازية وقطر الشريان العائد المعلمات مثل سرعة موجة النبض، ومؤشر زيادة، والضغوط النبض والامتثال. ويتم إنجاز الحصول على البيانات باستخدام برنامج كمبيوتر واحد لتحليل طوال مدة التجربة. يوفر هذه البيانات لا تقدر بثمن نظرة ثاقبة TEV مصفوفة إعادة عرض، resemblan لهام إلى الأم الضوابط / صورية والأداء TEV الكلي في الجسم الحي.

خضعت أكثر من 30 الأغنام تقنية جراحية وصفها في هذا التقرير لغرس TEVs (تحت الطبع) 9. يتم عرض جدول يلخص عمليات الأغنام أحدث بعد بروتوكول الأمثل في الجدول 2. جميع الأغنام تعافى بعد TEV زرع مع أي مضاعفات تهدد الحياة. في بعض الحيوانات، لوحظ تليف في الشريان الأصلي با?...

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Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring

بروتوكول خطوة بخطوة لوضع بين الموضعية من الأنسجة المهندسة سفن (TEVs) في الشريان السباتي من الأغنام باستخدام نهاية إلى نهاية مفاغرة في الوقت الحقيقي والتقييم الرقمي في الجسم الحي حتى التضحية الحيوانية.

تقنية جراحية لزرع الأنسجة المهندسة الأوعية الدموية الطعوم واللاحقة<em&gt; في فيفو</em&gt; الرصد

The development of Tissue Engineered Vessels (TEVs) is advanced by the ability to routinely and effectively implant TEVs (4-5 mm in diameter) into a large ...

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Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring

11.6K Views.  State University of New York Buffalo School of Medicine.  بروتوكول خطوة بخطوة لوضع بين الموضعية من الأنسجة المهندسة سفن (TEVs) في الشريان السباتي من الأغنام باستخدام نهاية إلى نهاية مفاغرة في الوقت الحقيقي والتقييم الرقمي في الجسم الحي حتى التضحية الحيوانية. 

Video: Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring

Bioluminescence Imaging for Assessment of Immune Responses Following Implantation of Engineered Heart Tissue (EHT)

Various techniques of cardiac tissue engineering have been pursued in the past decades including scaffolding strategies using either native or bioartificial scaffold materials, entrapment of cardiac myocytes in hydrogels such as fibrin or collagen and stacking of myocyte monolayers 1. These concepts aim at restoration of compromised cardiac function (e.g. after myocardial infarction) or as experimental models (e.g. predictive toxicology and substance screening or disease modelling). Precise monitoring of cell survival after implantation of engineered heart tissue (EHT) has now become possible using in-vivo bioluminescence imaging (BLI) techniques 2. Here we describe the generation of fibrin-based EHT from a transgenic rat strain with ubiquitous expression of firefly luciferase (ROSA/luciferase-LEW Tg; 3). Implantation is performed into the greater omentum of different rat strains to assess immune responses of the recipient organism following EHT implantation. Comparison of results generated by BLI and the Enzyme Linked Immuno Spot Technique (ELISPOT) confirm the usability of BLI for the assessment of immune responses.

Bioluminescence Imaging for Assessment of Immune Responses Following Implantation of Engineered Heart Tissue EHT

This video demonstrates the use of in vivo bioluminescence imaging to study immune responses after implantation of Engineered Heart Tissue (EHT) in rats.

التصوير تلألؤ بيولوجي لتقييم الاستجابات المناعية عقب زرع أنسجة القلب المهندسة (EHT)

Various techniques of cardiac tissue engineering have been pursued in the past decades including scaffolding strategies using either native or ...

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

Much effort has been devoted to develop and advance the methodology to regenerate functional small-diameter arterial bypasses. In the physiological environment, both mechanical and chemical stimulation are required to maintain the proper development and functionality of arterial vessels1,2.
Bioreactor culture systems developed by our group are designed to support vessel regeneration within a precisely controlled chemo-mechanical environment mimicking that of native vessels. Our bioreactor assembly and maintenance procedures are fairly simple and highly repeatable3,4. Smooth muscle cells (SMCs) are seeded onto a tubular polyglycolic acid (PGA) mesh that is threaded over compliant silicone tubing and cultured in the bioreactor with or without pulsatile stimulation for up to 12 weeks. There are four main attributes that distinguish our bioreactor from some predecessors. 1) Unlike other culture systems that simulate only the biochemical surrounding of native blood vessels, our bioreactor also creates a physiological pulsatile environment by applying cyclic radial strain to the vessels in culture. 2) Multiple engineered vessels can be cultured simultaneously under different mechanical conditions within a controlled chemical environment. 3) The bioreactor allows a mono layer of endothelial cells (EC) to be easily coated onto the luminal side of engineered vessels for animal implantation models. 4) Our bioreactor can also culture engineered vessels with different diameter size ranged from 1 mm to 3 mm, saving the effort to tailor each individual bioreactor to fit a specific diameter size. 
The engineered vessels cultured in our bioreactor resemble native blood vessels histologically to some degree. Cells in the vessel walls express mature SMC contractile markers such as smooth muscle myosin heavy chain (SMMHC)3. A substantial amount of collagen is deposited within the extracellular matrix, which is responsible for ultimate mechanical strength of the engineered vessels5. Biochemical analysis also indicates that collagen content of engineered vessels is comparable to that of native arteries6. Importantly, the pulsatile bioreactor has consistently regenerated vessels that exhibit mechanical properties that permit successful implantation experiments in animal models3,7. Additionally, this bioreactor can be further modified to allow real-time assessment and tracking of collagen remodeling over time, non-invasively, using a non-linear optical microscopy (NLOM)8. To conclude, this bioreactor should serve as an excellent platform to study the fundamental mechanisms that regulate the regeneration of functional small-diameter vascular grafts.

Our group has developed a bioreactor culture system that mimics the physiological pulsatile stresses of the cardiovascular system to regenerate implantable small-diameter vascular grafts.

الطعوم الوعائية الهندسة البيولوجية ، عن طريق بناء مفاعل حيوي نابض

Much effort has been devoted to develop and advance the methodology to regenerate functional small-diameter arterial bypasses. In the physiological ...

Production, Characterization and Potential Uses of a 3D Tissue-engineered Human Esophageal Mucosal Model

The incidence of both esophageal adenocarcinoma and its precursor, Barrett&#8217;s Metaplasia, are rising rapidly in the western world. Furthermore esophageal adenocarcinoma generally has a poor prognosis, with little improvement in survival rates in recent years. These are difficult conditions to study and there has been a lack of suitable experimental platforms to investigate disorders of the esophageal mucosa.
A model of the human esophageal mucosa has been developed in the MacNeil laboratory which, unlike conventional 2D cell culture systems, recapitulates the cell-cell and cell-matrix interactions present in vivo and produces a mature, stratified epithelium similar to that of the normal human esophagus. Briefly, the model utilizes non-transformed normal primary human esophageal fibroblasts and epithelial cells grown within a porcine-derived acellular esophageal scaffold. Immunohistochemical characterization of this model by CK4, CK14, Ki67 and involucrin staining demonstrates appropriate recapitulation of the histology of the normal human esophageal mucosa.
This model provides a robust, biologically relevant experimental model of the human esophageal mucosa. It can easily be manipulated to investigate a number of research questions including the effectiveness of pharmacological agents and the impact of exposure to environmental factors such as alcohol, toxins, high temperature or gastro-esophageal refluxate components. The model also facilitates extended culture periods not achievable with conventional 2D cell culture, enabling, inter alia, the study of the impact of repeated exposure of a mature epithelium to the agent of interest for up to 20 days. Furthermore, a variety of cell lines, such as those derived from esophageal tumors or Barrett&#8217;s Metaplasia, can be incorporated into the model to investigate processes such as tumor invasion and drug responsiveness in a more biologically relevant environment.

This manuscript describes the production, characterization and potential uses of a tissue engineered 3D esophageal construct prepared from normal primary human esophageal fibroblast and squamous epithelial cells seeded within a de-cellularized porcine scaffold. The results demonstrate the formation of a mature stratified epithelium similar to the normal human esophagus.

إنتاج وتوصيف والمحتملة استخدامات الأنسجة المهندسة-3D الإنسان المريء المخاطي نموذج

The incidence of both esophageal adenocarcinoma and its precursor, Barrett&#8217;s Metaplasia, are rising rapidly in the western world. Furthermore ...

Athymic Rat Model for Evaluation of Engineered Anterior Cruciate Ligament Grafts

Anterior cruciate ligament (ACL) rupture is a common ligamentous injury that often requires surgery because the ACL does not heal well without intervention. Current treatment strategies include ligament reconstruction with either autograft or allograft, which each have their associated limitations. Thus, there is interest in designing a tissue-engineered graft for use in ACL reconstruction. We describe the fabrication of an electrospun polymer graft for use in ACL tissue engineering. This polycaprolactone graft is biocompatible, biodegradable, porous, and is comprised of aligned fibers. Because an animal model is necessary to evaluate such a graft, this paper describes an intra-articular athymic rat model of ACL reconstruction that can be used to evaluate engineered grafts, including those seeded with xenogeneic cells. Representative histology and biomechanical testing results at 16 weeks postoperatively are presented, with grafts tested immediately post-implantation and contralateral native ACLs serving as controls. The present study provides a reproducible animal model with which to evaluate tissue engineered ACL grafts, and demonstrates the potential of a regenerative medicine approach to treatment of ACL rupture.

Animal models are important tools for the evaluation of tissue-engineered grafts. This paper presents the protocol for preparing an electrospun biodegradable polymer graft for use in anterior cruciate ligament tissue engineering, as well as a surgical protocol for implantation in a rat model.

Athymic الجرذ نموذج لتقييم المهندسة الرباط الصليبي الأمامي الطعوم

Anterior cruciate ligament (ACL) rupture is a common ligamentous injury that often requires surgery because the ACL does not heal well without ...

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Synthetic materials are known to initiate clinical complications such as inflammation, stenosis, and infections when implanted as vascular substitutes. Collagen has been extensively used for a wide range of biomedical applications and is considered a valid alternative to synthetic materials due to its inherent biocompatibility (i.e., low antigenicity, inflammation, and cytotoxic responses). However, the limited mechanical properties and the related low hand-ability of collagen gels have hampered their use as scaffold materials for vascular tissue engineering. Therefore, the rationale behind this work was first to engineer cellularized collagen gels into a tubular-shaped geometry and second to enhance smooth muscle cells driven reorganization of collagen matrix to obtain tissues stiff enough to be handled.
The strategy described here is based on the direct assembling of collagen and smooth muscle cells (construct) in a 3D cylindrical geometry with the use of a molding technique. This process requires a maturation period, during which the constructs are cultured in a bioreactor under static conditions (without applied external dynamic mechanical constraints) for 1 or 2 weeks. The &#8220;static bioreactor&#8221; provides a monitored and controlled sterile environment (pH, temperature, gas exchange, nutrient supply and waste removal) to the constructs. During culture period, thickness measurements were performed to evaluate the cells-driven remodeling of the collagen matrix, and glucose consumption and lactate production rates were measured to monitor the cells metabolic activity. Finally, mechanical and viscoelastic properties were assessed for the resulting tubular constructs. To this end, specific protocols and a focused know-how (manipulation, gripping, working in hydrated environment, and so on) were developed to characterize the engineered tissues.

In this work, we present a technique for the rapid fabrication of living vascular tissues by direct culturing of collagen, smooth muscle cells and endothelial cells. In addition, a new protocol for the mechanical characterization of engineered vascular tissues is described.

الهندسة 3D Cellularized الهلام الكولاجين لتجديد الأنسجة الوعائية

Synthetic materials are known to initiate clinical complications such as inflammation, stenosis, and infections when implanted as vascular substitutes. ...

Applications of In Vivo Functional Testing of the Rat Tibialis Anterior for Evaluating Tissue Engineered Skeletal Muscle Repair

Despite the regenerative capacity of skeletal muscle, permanent functional and/or cosmetic deficits (e.g., volumetric muscle loss (VML) resulting from traumatic injury, disease and various congenital, genetic and acquired conditions are quite common. Tissue engineering and regenerative medicine technologies have enormous potential to provide a therapeutic solution. However, utilization of biologically relevant animal models in combination with longitudinal assessments of pertinent functional measures are critical to the development of improved regenerative therapeutics for treatment of VML-like injuries. In that regard, a commercial muscle lever system can be used to measure length, tension, force and velocity parameters in skeletal muscle. We used this system, in conjunction with a high power, bi-phase stimulator, to measure in vivo force production in response to activation of the anterior crural compartment of the rat hindlimb. We have previously used this equipment to assess the functional impact of VML injury on the tibialis anterior (TA) muscle, as well as the extent of functional recovery following treatment of the injured TA muscle with our tissue engineered muscle repair (TEMR) technology. For such studies, the left foot of an anaesthetized rat is securely anchored to a footplate linked to a servomotor, and the common peroneal nerve is stimulated by two percutaneous needle electrodes to elicit muscle contraction and dorsiflexion of the foot. The peroneal nerve stimulation-induced muscle contraction is measured over a range of stimulation frequencies (1-200 Hz), to ensure an eventual plateau in force production that allows for an accurate determination of peak tetanic force. In addition to evaluation of the extent of VML injury as well as the degree of functional recovery following treatment, this methodology can be easily applied to study diverse aspects of muscle physiology and pathophysiology. Such an approach should assist with the more rational development of improved therapeutics for muscle repair and regeneration.

Applications of In Vivo Functional Testing of the Rat Tibialis Anterior for Evaluating Tissue Engineered Skeletal Muscle Repair

We describe an in vivo protocol to measure dorsiflexion of the foot following stimulation of the peroneal nerve and contraction of the anterior crural compartment of the rat hindlimb. Such measurements are an indispensable translational tool for evaluating skeletal muscle pathology and tissue engineering approaches to muscle repair and regeneration.

تطبيقات<em&gt; في فيفو</em&gt; التجارب الفنية من الفئران قصبة الساق الأمامية لتقييم الأنسجة المهندسة وإصلاح العضلات والهيكل العظمي

Despite the regenerative capacity of skeletal muscle, permanent functional and/or cosmetic deficits (e.g., volumetric muscle loss (VML) resulting from ...

Scaling of Engineered Vascular Grafts Using 3D Printed Guides and the Ring Stacking Method

Coronary artery disease remains a leading cause of death, affecting millions of Americans. With the lack of autologous vascular grafts available, engineered grafts offer great potential for patient treatment. However, engineered vascular grafts are generally not easily scalable, requiring manufacture of custom molds or polymer tubes in order to customize to different sizes, constituting a time-consuming and costly practice. Human arteries range in lumen diameter from about 2.0-38 mm and in wall thickness from about 0.5-2.5 mm. We have created a method, termed the "Ring Stacking Method," in which variable size rings of tissue of the desired cell type, demonstrated here with vascular smooth muscle cells (SMCs), can be created using guides of center posts to control lumen diameter and outer shells to dictate vessel wall thickness. These tissue rings are then stacked to create a tubular construct, mimicking the natural form of a blood vessel. The vessel length can be tailored by simply stacking the number of rings required to constitute the length needed. With our technique, tissues of tubular forms, similar to a blood vessel, can be readily manufactured in a variety of dimensions and lengths to meet the needs of the clinic and patient.

Scalable engineered blood vessels would improve clinical applicability. Using easily sizable 3D-printed guides, rings of vascular smooth muscle were created and stacked into a tubular form, forming a vascular graft. Grafts can be sized to meet the range of human coronary artery dimensions by simply changing the 3D-printed guide size.

التحجيم من المهندسة الطعوم الأوعية الدموية عن طريق 3D مطبوعة أدلة والطوق زرع الطريقة

Coronary artery disease remains a leading cause of death, affecting millions of Americans. With the lack of autologous vascular grafts available, ...

A Novel Tenorrhaphy Suture Technique with Tissue Engineered Collagen Graft to Repair Large Tendon Defects

Surgical management of large tendon defects with tendon grafts is challenging, as there are a finite number of sites where donors can be readily identified and used. Currently, this gap is filled with tendon auto-, allo-, xeno-, or artificial grafts, but clinical methods to secure them are not necessarily translatable to animals because of the scale. In order to evaluate new biomaterials or study a tendon graft made up of collagen type 1, we have developed a modified suture technique to help maintain the engineered tendon in alignment with the tendon ends. Mechanical properties of these grafts are inferior to the native tendon. To incorporate engineered tendon into clinically relevant models of loaded repair, a strategy was adopted to offload the tissue engineered tendon graft and allow for the maturation and integration of the engineered tendon in vivo until a mechanically sound neo-tendon was formed. We describe this technique using incorporation of the collagen type 1 tissue engineered tendon construct.

In this paper, we present an in vitro and in situ protocol to repair a tendon gap of up to 1.5 cm by filling it with engineered collagen graft. This was performed by developing a modified suture technique to take the mechanical load until the graft matures into the host tissue.

تقنية خياطة تينورهافي جديدة مع الأنسجة المهندسة الكولاجين الكسب غير المشروع لإصلاح عيوب وتر كبير

Surgical management of large tendon defects with tendon grafts is challenging, as there are a finite number of sites where donors can be readily ...

A Novel Surgical Technique As a Foundation for In Vivo Partial Liver Engineering in Rat

Organ engineering is a novel strategy to generate liver organ substitutes that can potentially be used in transplantation. Recently, in vivo liver engineering, including in vivo organ decellularization followed by repopulation, has emerged as a promising approach over ex vivo liver engineering. However, postoperative survival was not achieved. The aim of this study is to develop a novel surgical technique of in vivo selective liver lobe perfusion in rats as a prerequisite for in vivo liver engineering. We generate a circuit bypass only through the left lateral lobe. Then, the left lateral lobe is perfused with heparinized saline. The experiment is performed with 4 groups (n = 3 rats per group) based on different perfusion times of 20 min, 2 h, 3 h, and 4 h. Survival, as well as the macroscopically visible change of color and the histologically determined absence of blood cells in the portal triad and the sinusoids, is taken as an indicator for a successful model establishment. After selective perfusion of the left lateral lobe, we observe that the left lateral lobe, indeed, turned from red to faint yellow. In a histological assessment, no blood cells are visible in the branch of the portal vein, the central vein, and the sinusoids. The left lateral lobe turns red after reopening the blocked vessels. 12/12 rats survived the procedure for more than one week. We are the first to report a surgical model for in vivo single liver lobe perfusion with a long survival period of more than one week. In contrast to the previously published report, the most important advantage of the technique presented here is that perfusion of 70% of the liver is maintained throughout the whole procedure. The establishment of this technique provides a foundation for in vivo partial liver engineering in rats, including decellularization and recellularization.

A Novel Surgical Technique As a Foundation for In Vivo Partial Liver Engineering in Rat

We establish a novel surgical technique for an in vivo single liver lobe perfusion model in rat as a prerequisite for further studying in vivo partial liver engineering in the future.

رواية تقنية جراحية كأساس في فيفو جزئي الهندسة الكبد في الفئران

Organ engineering is a novel strategy to generate liver organ substitutes that can potentially be used in transplantation. Recently, in vivo liver ...

Nondestructive Monitoring of Degradable Scaffold-Based Tissue-Engineered Blood Vessel Development Using Optical Coherence Tomography

Engineered vascular grafts with structural and mechanical properties similar to natural blood vessels are expected to meet the growing demand for arterial bypass. Characterization of the growth dynamics and remodeling process of degradable polymer scaffold-based tissue-engineered blood vessels (TEBVs) with pulsatile stimulation is crucial for vascular tissue engineering. Optical imaging techniques stand out as powerful tools for monitoring vascularization of engineered tissue enabling high-resolution imaging in real-time culture. This paper demonstrates a nondestructive and fast real-time imaging strategy to monitor the growth and remodeling of TEBVs in long-term culture by using optical coherence tomography (OCT). Geometric morphology is evaluated, including vascular remodeling process, wall thickness, and comparison of TEBV thickness in different culture time points and presence of pulsatile stimulation. Finally, OCT provides practical possibilities for real-time observation of the degradation of polymer in the reconstructing tissues under pulsatile stimulation or not and in each vessel segment, by compared with the assessment of polymer degradation using scanning electron microscopic(SEM) and polarized microscope.

A step by step protocol for nondestructive and long-period monitoring the process of vascular remodeling and scaffold degradation in real-time culture of biodegradable polymeric scaffold-based tissue-engineered blood vessels with pulsatile stimulation using optical coherence tomography is described here.

رصد غير تدميري التنمية القابلة للتحلل القائم على سقالة هندسيا أنسجة الأوعية الدموية باستخدام التصوير المقطعي التماسك البصري

Engineered vascular grafts with structural and mechanical properties similar to natural blood vessels are expected to meet the growing demand for arterial ...