وقد تم تمويل هذا العمل من قبل منحة المعاهد الوطنية للصحة R01AI077610.

وأجريت كافة الإجراءات وفقا لدليل لرعاية واستخدام الحيوانات المختبرية من المعهد الوطني للصحة (NIH) وتمت الموافقة من قبل لجنة جامعة جونز هوبكنز رعاية الحيوان واستخدام (JHUACUC). تم تنفيذ إجراءات محددة في إطار MO13M292 بروتوكولات ACUC المعتمدة وMO13M370. 1. المانحة الجلد الحصاد <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> تخدير الماوس المانحة مع isoflurane (المرذاذ الحث على 4٪ والصيانة في 1-2٪ من خلال مخروط الماوس). استخدام إصبع القدم انسحاب قرصة العاكسة لرصد عمق التخدير. </li><li style=";text-align:right;direction:rtl"> باستخدام الحلاقة الكهربائية، حلاقة الجزء الخلفي من الحيوان وتعقيمها مع 10٪ اليود البوفيدون (على سبيل المثال، Betadine). </li><li style=";text-align:right;direction:rtl"> باستخدام مقص العقيمة، والقفازات، وتقنية العقيم، والحصاد المانحة الجلد الخلفي من الفخذ في الرقبة، مع تشريح حادة على مستوى النسيج الضام الهالي. </li><li style=";text-align:right;direction:rtl"> الموت ببطء الحيوانية خلع عنق الرحم بعد حصادرقعة جلدية. </li><li style=";text-align:right;direction:rtl"> تحت المجهر، فصل الأنسجة الضامة والأنسجة الدهنية، وعضلية السبلة من الجلد مرة أخرى باستخدام مقص بضع وتر الجميلة. وعضلية السبلة هو رقيقة، شفافة العضلات المسؤولة عن حركة الوخز الجلد. </li><li style=";text-align:right;direction:rtl"> استخدام معقم أدوات تقنية، قطع ترقيع 15 ملم × 15 ملم من الجلد مرة أخرى ل10 ملم × 10 ملم إلى 15 ملم × 15 ملم السرير الكسب غير المشروع. </li><li style=";text-align:right;direction:rtl"> تخزين الطعوم على الشاش غارقة العقيمة مع الفوسفات مخزنة المالحة (PBS) في طبق بتري على الجليد. ملاحظة: 8-10 الطعوم يمكن الحصول عليها من الماوس مانح واحد. </li></ol> 2. مستلم الجلد زرع <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> تخدير الماوس المتلقي مع isoflurane (المرذاذ الحث على 4٪ والصيانة في 1-2٪ من خلال مخروط الماوس). استخدام إصبع القدم انسحاب قرصة العاكسة لرصد عمق التخدير. </li><li style=";text-align:right;direction:rtl"> إدارة 0.02 ملغم / كجم من وزن الجسم من البوبرينورفين لتخفيف الألم بعد الجراحة. </li><li style=";text-align:right;direction:rtl"> حلق الجانب من الجزء الخلفي من الحيوان حيث واي الكسب غير المشروعليرة لبنانية تكون إدراج وتعقيم مع 10٪ البوفيدون اليود. </li><li style=";text-align:right;direction:rtl"> باستخدام مقص، وقطع 10 ملم × 10 ملم إلى 15 ملم × 15 ملم مربع من الجلد. يجب أن يكون حجم الخلل أكبر قليلا (10٪) من الكسب غير المشروع. قطع كما ظاهريا ممكن. الحرص على الحفاظ على عضلية السبلة والسفن. وعضلية السبلة يمكن تمييزها من اللفافة الكامنة التي كتبها التنقل والأوعية الدموية التي تعمل سطحية لذلك. </li><li style=";text-align:right;direction:rtl"> ضع الكسب غير المشروع على السرير الكسب غير المشروع، وتجنب طيات على طول الحواف. </li><li style=";text-align:right;direction:rtl"> وضع 8 الغرز على الزوايا وعلى منتصف كل طرف. لكل خياطة، وتمرير الإبرة من خلال الكسب غير المشروع ومن ثم عن طريق عضلية السبلة من الكسب غير المشروع سرير تحت الجلد المتلقي المحيطة بها. </li><li style=";text-align:right;direction:rtl"> إزالة القناع مخدر والسماح للحيوان يتعافى جزئيا من التخدير قبل تطبيق ضمادة لاصقة. </li><li style=";text-align:right;direction:rtl"> التفاف الماوس المتلقي في ضمادة لاصقة مع الشاش مطوية على الكسب غير المشروع. جعل ضمادة من خلال الجمع بين اثنين من الضمادات، وقطعالجزء اللاصق من واحد ووضع منصات ماصة اثنين معا. </li><li style=";text-align:right;direction:rtl"> مراقبة الماوس عن كثب خلال الانتعاش للتأكد من أن ضمادة لا تقيد الصدر سياحة والتنفس. إزالة الضمادة إذا يقلل من معدل التنفس أو يبدأ الحيوان في اللحظات أو التنفس بسطحية. </li></ol> 3. بعد العملية الجراحية العناية <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> إدارة إينروفلوكساسين 5 ملغ / كغ بعد عملية جراحية للوقاية من العدوى. </li><li style=";text-align:right;direction:rtl"> ضع الماوس المزروعة في قفص نظيفة على وسادة التدفئة ميكرووافابل حتى يتعافى تماما من التخدير. </li><li style=";text-align:right;direction:rtl"> مراقبة الماوس لمدة 1 ساعة بعد العمل الجراحي قبل إعادته إلى منشأة سكنية. </li><li style=";text-align:right;direction:rtl"> بعد سبعة أيام من الجراحة، تخدير الماوس كما في الخطوة 2.1. إزالة الضمادة عن طريق قطع فقط من خلال الجانب البطني من ضمادة. </li><li style=";text-align:right;direction:rtl"> مراقبة وجس الكسب غير المشروع في اليوم التالي لعلامات scabbing، الانكماش، أو صلابة. إذا كان موجودا، والكسب غير المشروع قد لا يتحقق vasculariza السليموينبغي النظر في نشوئها وعطل فني. </li><li style=";text-align:right;direction:rtl"> مراقبة يوميا بحثا عن علامات على الرفض. النظر في ترقيع رفض عندما ≥90٪ من الأنسجة الكسب غير المشروع يصبح الميتة. </li><li style=";text-align:right;direction:rtl"> الموت ببطء الحيوانات رفضت وحصادها لتحليلها. </li></ol>

<ol>
	<li>Furtmuller, G. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Orthotopic+Hind+Limb+Transplantation+in+the+Mouse.">Orthotopic Hind Limb Transplantation in the Mouse.</a> J Vis Exp. , (2016).</li><li>Oh, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+Novel+Microsurgical+Model+for+Heterotopic,+En+Bloc+Chest+Wall,+Thymus,+and+Heart+Transplantation+in+Mice.">A Novel Microsurgical Model for Heterotopic, En Bloc Chest Wall, Thymus, and Heart Transplantation in Mice.</a> J Vis Exp. , (2016).</li><li>Oberhuber, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Murine+cervical+heart+transplantation+model+using+a+modified+cuff+technique.">Murine cervical heart transplantation model using a modified cuff technique.</a> J Vis Exp. , e50753 (2014).</li><li>Jones, T. R., Shirasugi, N., Adams, A. B., Pearson, T. C., Larsen, C. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intravital+microscopy+identifies+selectins+that+regulate+T+cell+traffic+into+allografts.">Intravital microscopy identifies selectins that regulate T cell traffic into allografts.</a> J Clin Invest. 112, 1714-1723 (2003).</li><li>Celli, S., Albert, M. L., Bousso, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Visualizing+the+innate+and+adaptive+immune+responses+underlying+allograft+rejection+by+two-photon+microscopy.">Visualizing the innate and adaptive immune responses underlying allograft rejection by two-photon microscopy.</a> Nat Med. 17, 744-749 (2011).</li><li>Medawar, P. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+behaviour+and+fate+of+skin+autografts+and+skin+homografts+in+rabbits:+A+report+to+the+War+Wounds+Committee+of+the+Medical+Research+Council.">The behaviour and fate of skin autografts and skin homografts in rabbits: A report to the War Wounds Committee of the Medical Research Council.</a> J Anat. 78, 176-199 (1944).</li><li>Billingham, R. E., Brent, L., Medawar, P. B., Sparrow, E. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantitative+studies+on+tissue+transplantation+immunity.+I.+The+survival+times+of+skin+homografts+exchanged+between+members+of+different+inbred+strains+of+mice.">Quantitative studies on tissue transplantation immunity. I. The survival times of skin homografts exchanged between members of different inbred strains of mice.</a> Proc R Soc Lond B Biol Sci. 143, 43-58 (1954).</li><li>Garrod, K. R., Cahalan, M. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Murine+skin+transplantation.">Murine skin transplantation.</a> J Vis Exp. , (2008).</li><li>Schmaler, M., Broggi, M. A., Rossi, S. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transplantation+of+tail+skin+to+study+allogeneic+CD4+T+cell+responses+in+mice.">Transplantation of tail skin to study allogeneic CD4 T cell responses in mice.</a> J Vis Exp. , e51724 (2014).</li><li>Bergstresser, P. R., Toews, G. B., Gilliam, J. N., Streilein, J. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Unusual+numbers+and+distribution+of+Langerhans+cells+in+skin+with+unique+immunologic+properties.">Unusual numbers and distribution of Langerhans cells in skin with unique immunologic properties.</a> J Invest Dermatol. 74, 312-314 (1980).</li><li>Chen, H. D., Silvers, W. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Influence+of+Langerhans+cells+on+the+survival+of+H-Y+incompatible+skin+grafts+in+rats.">Influence of Langerhans cells on the survival of H-Y incompatible skin grafts in rats.</a> J Invest Dermatol. 81, 20-23 (1983).</li><li>Chong, A. S., Alegre, M. L., Miller, M. L., Fairchild, R. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lessons+and+limits+of+mouse+models.">Lessons and limits of mouse models.</a> Cold Spring Harb Perspect Med. 3, a015495 (2013).</li><li>Mayumi, H., Nomoto, K., Good, R. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+surgical+technique+for+experimental+free+skin+grafting+in+mice.">A surgical technique for experimental free skin grafting in mice.</a> Jpn J Surg. 18, 548-557 (1988).</li><li>McFarland, H. I., Rosenberg, A. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Skin+allograft+rejection.">Skin allograft rejection.</a> Curr Protoc Immunol. Chapter 4, (2009).</li><li>Lee, C. F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Preventing+Allograft+Rejection+by+Targeting+Immune+Metabolism.">Preventing Allograft Rejection by Targeting Immune Metabolism.</a> Cell reports. 13, 760-770 (2015).</li><li>Pollizzi, K. N., Powell, J. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Integrating+canonical+and+metabolic+signalling+programmes+in+the+regulation+of+T+cell+responses.">Integrating canonical and metabolic signalling programmes in the regulation of T cell responses.</a> Nat Rev Immunol. 14, 435-446 (2014).</li><li>Pearce, E. L., Poffenberger, M. C., Chang, C. H., Jones, R. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fueling+immunity:+insights+into+metabolism+and+lymphocyte+function.">Fueling immunity: insights into metabolism and lymphocyte function.</a> Science. 342, 1242454 (2013).</li></ol>

الكتاب ليس لديهم ما يكشف.

منذ مقدمته التي كتبها مدور، الأول في الدراسات الإنسانية وثم في الأرانب والفئران، وقد تم زرع الجلد نموذج لا تقدر بثمن لدراسة الاستجابات المناعية خيفي 6،7. في هذه المخطوطة، فإننا نقدم نموذجا على نطاق واسع، غير أوعية دموية، وزرع الجلد كامل سماكة باستخدام الجلد ال?...

زرع الأعضاء هو الاختيار الامثل لعلاج المرضى الذين يعانون من فشل في نهاية المرحلة الجهاز، والنتائج قد تحسنت بشكل ملحوظ مع التقدم في العمليات الجراحية والبروتوكولات المناعة. ومع ذلك، ويرتبط كبت المناعة على المدى الطويل مع آثار جانبية كبيرة، ووضع استراتيجيات جديدة تعزز التسامح يبقى الهدف من البحوث زرع الحديث. وقد وضعت نماذج حيوانية عديدة للبحوث الأساسية في زراعة الأعضاء، لدراسة آليات الرفض المزروع واختبار النهج كبت المناعة لمنع رفض الكسب غير المشروع وتعزيز التسامح على المدى الطويل 1-3. أصبحت نماذج الماوس الدعامة الأساسية لبحوث المناعة نظرا لتوافر الحصري واسعة من الأجسام المضادة التشخيصية والعلاجية واضحة المعالم السلالات الفطرية والمعدلة وراثيا. زرع الجلد هو إجراء بسيط لا يتطلب مهارات المجهرية الخاصة ويمكن رصدها بسهولة بعد العمل الجراحي. مجتمعة، كان زرع الجلد الماوس أداة استثنائية لدراسة العديد من الجوانب المعنية في الاستجابة منيع للمستضد الخيفي، بما في ذلك تقديم المستضد والاتجار الخلية، وتدمير الأنسجة أثناء الكسب غير المشروع الرفض 4،5. هنا، نقدم لك مجموعة من الإجراء خطوة بخطوة لزرع الجلد كامل سماكة باستخدام نموذج الفأر، ونحن تصف الخطوات الهامة اللازمة لengraftment ناجحة من الجلد المزروع.

<table><tbody><tr><td>Straight micro forceps</td><td>Sigma</td><td>F4017</td><td></td></tr><tr><td>Curved micro forceps</td><td>Aesculap</td><td>BD333R</td><td></td></tr><tr><td>Curved Stevens tenotomy scissors</td><td>Aesculap</td><td>BC905R</td><td></td></tr><tr><td>Mayo dissecting scissors</td><td>Sigma</td><td>S3146</td><td></td></tr><tr><td>Micro needle holder</td><td>Aesculap</td><td>BM563R</td><td></td></tr><tr><td>Sterile gauze</td><td>Covidien</td><td>441218</td><td></td></tr><tr><td>6-0 Nylon suture</td><td>MWI</td><td>31849</td><td></td></tr><tr><td>Plastic Strips Band&amp;#45;Aid</td><td>Johnson &amp;amp; Johnson</td><td></td><td>Obtained from pharmacy</td></tr><tr><td>10 cm Petri dish</td><td>Fisherbrand</td><td>FB0875712</td><td></td></tr><tr><td>PBS</td><td>Quality Biological</td><td>119069131</td><td></td></tr><tr><td>Buprenorphine</td><td></td><td></td><td>DEA Number required; Obtained from hospital pharmacy</td></tr><tr><td>Enrofloxacin</td><td>Bayer Health Care</td><td>186599</td><td></td></tr></tbody></table>

murine full-thickness skin transplantation

الفئران كامل سمك زرع الجلد هو راسخة في الجسم الحي نموذج لدراسة منيع للمستضد الخيفي الاستجابة والرفض الكسب غير المشروع. وعلى الرغم من تطبيق محدود للبشر، وزرع الجلد في الفئران وقد استخدمت على نطاق واسع للبحث الزرع. هذا الإجراء هو سهلة التعلم وأداء، وأنها لا تتطلب تقنيات المجهرية الدقيقة ولا تدريب مكثف. وعلاوة على ذلك، رفض الكسب غير المشروع في هذا النموذج يحدث في رد فعل مناعي استنساخه جدا ويتم رصد بسهولة عن طريق التفتيش المباشر والجس. وبالإضافة إلى ذلك، زرع الجلد الثانوي مع الطعوم المطابقة المانحة أو الجلد طرف ثالث يمكن أن يؤديها على المزيد من النماذج زرع معقدة كبديل وطريقة غير معقدة لتقييم التسامح المانحين محددة. مضاعفات منخفضة وهي بشكل عام تقتصر على جرعة زائدة من المخدر أو ضيق في التنفس بعد العملية. فشل الكسب غير المشروع، من ناحية أخرى، يحدث عادة نتيجة لضعف الاستعدادات للغرامفي الخلف، وتحديد المواقع غير صحيحة في السرير الكسب غير المشروع، أو وضع غير مناسب للضمادة. في هذه المقالة، نقدم بروتوكول لكامل سماكة زرع الجلد في الفئران ووصف الخطوات الهامة اللازمة لإجراء ناجحة.

وضع ضمادة على الماوس المتلقي هو خطوة هامة من هذا الإجراء. يتم وضع الكسب غير المشروع الجلد على الجذع المتلقي، بين الكتف والورك والعمود الفقري (الشكل 1). ويتكون ضمادة الشاش مع مطوية والجمع بين اثنين من ضمادات لاصقة البلاستيكية. يتم وضع الماوس ?...

Watch this Scientific Journal Video about Murine Full-thickness Skin Transplantation at JoVE.com

Murine Full-thickness Skin Transplantation

الفئران كامل سمك زرع الجلد هو نموذج راسخة لدراسة الرفض في مكان منيع للمستضد الخيفي. هنا، ونحن نقدم البرنامج التعليمي كل خطوة تشارك في أداء BALB / ج -&gt; C57BL / 6 زرع الجلد كامل سماكة.

الفئران كامل سماكة الجلد زراعة

Murine full-thickness skin transplantation is a well-established in vivo model to study alloimmune response and graft rejection. Despite its limited ...

murine-full-thickness-skin-transplantation

Research

JoVE Journal

Immunology and Infection

20.0K Views.  Johns Hopkins University School of Medicine.  الفئران كامل سمك زرع الجلد هو نموذج راسخة لدراسة الرفض في مكان منيع للمستضد الخيفي. هنا، ونحن نقدم البرنامج التعليمي كل خطوة تشارك في أداء BALB / ج -> C57BL / 6 زرع الجلد كامل سماكة. 

Video: Murine Full-thickness Skin Transplantation

The Three-Dimensional Human Skin Reconstruct Model: a Tool to Study Normal  Skin and Melanoma Progression

Most in vitro studies in experimental skin biology have been done in 2-dimensional (2D) monocultures, while accumulating evidence suggests that cells behave differently when they are grown within a 3D extra-cellular matrix and also interact with other cells (1-5). Mouse models have been broadly utilized to study tissue morphogenesis in vivo. However mouse and human skin have significant differences in cellular architecture and physiology, which makes it difficult to extrapolate mouse studies to humans. Since melanocytes in mouse skin are mostly localized in hair follicles, they have distinct biological properties from those of humans, which locate primarily at the basal layer of the epidermis. The recent development of 3D human skin reconstruct models has enabled the field to investigate cell-matrix and cell-cell interactions between different cell types. The reconstructs consist of a "dermis" with fibroblasts embedded in a collagen I matrix, an "epidermis", which is comprised of stratified, differentiated keratinocytes and a functional basement membrane, which separates epidermis from dermis. Collagen provides scaffolding, nutrient delivery, and potential for cell-to-cell interaction. The 3D skin models incorporating melanocytic cells recapitulate natural features of melanocyte homeostasis and melanoma progression in human skin. As in vivo, melanocytes in reconstructed skin are localized at the basement membrane interspersed with basal layer keratinocytes. Melanoma cells exhibit the same characteristics reflecting the original tumor stage (RGP, VGP and metastatic melanoma cells) in vivo. Recently, dermal stem cells have been identified in the human dermis (6). These multi-potent stem cells can migrate to the epidermis and differentiate to melanocytes.

In this report, we describe the three-dimensional skin reconstruct model which mimics human skin in architecture and composition. Melanocyte physiology, melanoma progression and the fate of dermal stem cells have been investigated using the skin reconstruct model. The model is also useful as a preclinical tool for drug assessment.

الجلد ثلاثي الأبعاد إعادة بناء الإنسان النموذجي : أداة لدراسة بشرة العادية والتقدم الميلانوما

Most in vitro studies in experimental skin biology have been done in 2-dimensional (2D) monocultures, while accumulating evidence suggests that cells ...

Bioengineering

Murine Skin Transplantation

As one of the most stringent and least technically challenging models, skin transplantation is a standard method to assay host T cell responses to MHC-disparate donor antigens. The aim of this video-article is to provide the viewer with a step-by-step visual demonstration of skin transplantation using the mouse model. The protocol is divided into 5 main components: 1) harvesting donor skin; 2) preparing recipient for transplant; 3) skin transplant; 4) bandage removal and monitoring graft rejection; 5) helpful hints. Once proficient, the procedure itself should take &lt;10 min to perform.

Allogeneic skin transplantation is a standard model to assay host T cell responses to MHC-disparate donor antigens. This video-article provides a visual tutorial of each step involved in performing a BALB/c--&gt;C57BL/6 skin transplant.

زرع الجلد الفئران

As one of the most stringent and least technically challenging models, skin transplantation is a standard method to assay host T cell responses to ...

Biology

A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo

Insufficient vascularization is considered to be one of the main factors limiting the clinical success of tissue-engineered constructs. In order to evaluate new strategies that aim at improving vascularization, reliable methods are required to make the in-growth of new blood vessels into bio-artificial scaffolds visible and quantify the results. Over the past couple of years, our group has introduced a full skin defect model that enables the direct visualization of blood vessels by transillumination and provides the possibility of quantification through digital segmentation. In this model, one surgically creates full skin defects in the back of mice and replaces them with the material tested. Molecules or cells of interest can also be incorporated in such materials to study their potential effect. After an observation time of one&#8217;s own choice, materials are explanted for evaluation. Bilateral wounds provide the possibility of making internal comparisons that minimize artifacts among individuals as well as of decreasing the number of animals needed for the study. In comparison to other approaches, our method offers a simple, reliable and cost effective analysis. We have implemented this model as a routine tool to perform high-resolution screening when testing vascularization of different biomaterials and bio-activation approaches.

A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo

Vascularization is key to approaches in successful tissue engineering. Therefore, reliable technologies are required to evaluate the development of vascular networks in tissue-constructs. Here we present a simple and cost-effective method to visualize and quantify vascularization in vivo.

جلد كامل عيب نموذج في تقييم الأوعية الدموية من الحيوية<em&gt; في فيفو</em

Insufficient vascularization is considered to be one of the main factors limiting the clinical success of tissue-engineered constructs. In order to ...

Murine Model of Wound Healing

Wound healing and repair are the most complex biological processes that occur in human life. After injury, multiple biological pathways become activated. Impaired wound healing, which occurs in diabetic patients for example, can lead to severe unfavorable outcomes such as amputation. There is, therefore, an increasing impetus to develop novel agents that promote wound repair. The testing of these has been limited to large animal models such as swine, which are often impractical. Mice represent the ideal preclinical model, as they are economical and amenable to genetic manipulation, which allows for mechanistic investigation. However, wound healing in a mouse is fundamentally different to that of humans as it primarily occurs via contraction. Our murine model overcomes this by incorporating a splint around the wound. By splinting the wound, the repair process is then dependent on epithelialization, cellular proliferation and angiogenesis, which closely mirror the biological processes of human wound healing. Whilst requiring consistency and care, this murine model does not involve complicated surgical techniques and allows for the robust testing of promising agents that may, for example, promote angiogenesis or inhibit inflammation. Furthermore, each mouse acts as its own control as two wounds are prepared, enabling the application of both the test compound and the vehicle control on the same animal. In conclusion, we demonstrate a practical, easy-to-learn, and robust model of wound healing, which is comparable to that of humans.

A murine model of cutaneous wound healing that can be used to assess therapeutic compounds in physiological and pathophysiological settings.

نموذج الفئران من التئام الجروح

Wound healing and repair are the most complex biological processes that occur in human life. After injury, multiple biological pathways become activated. ...

Medicine

Minced Tissue in Compressed Collagen: A Cell-containing Biotransplant for Single-staged Reconstructive Repair

Conventional techniques for cell expansion and transplantation of autologous cells for tissue engineering purposes can take place in specially equipped human cell culture facilities. These methods include isolation of cells in single cell suspension and several laborious and time-consuming events before transplantation back to the patient. Previous studies suggest that the body itself could be used as a bioreactor for cell expansion and regeneration of tissue in order to minimize ex vivo manipulations of tissues and cells before transplanting to the patient. The aim of this study was to demonstrate a method for tissue harvesting, isolation of continuous epithelium, mincing of the epithelium into small pieces and incorporating them into a three-layered biomaterial. The three-layered biomaterial then served as a delivery vehicle, to allow surgical handling, exchange of nutrition across the transplant, and a controlled degradation. The biomaterial consisted of two outer layers of collagen and a core of a mechanically stable and slowly degradable polymer. The minced epithelium was incorporated into one of the collagen layers before transplantation. By mincing the epithelial tissue into small pieces, the pieces could be spread and thereby the propagation of cells was stimulated. After the initial take of the transplants, cell expansion and reorganization would take place and extracellular matrix mature to allow ingrowth of capillaries and nerves and further maturation of the extracellular matrix. The technique minimizes ex vivo manipulations and allow cell harvesting, preparation of autograft, and transplantation to the patient as a simple one-stage intervention. In the future, tissue expansion could be initiated around a 3D mold inside the body itself, according to the specific needs of the patient. Additionally, the technique could be performed in an ordinary surgical setting without the need for sophisticated cell culturing facilities.

Tissue engineering often includes in vitro expansion in order to create autografts for tissue regeneration. In this study a method for tissue expansion, regeneration, and reconstruction in vivo was developed in order to minimize the processing of cells and biological materials outside the body.

الأنسجة المفروم في المضغوط الكولاجين: وBiotransplant التي تحتوي على خلية لإصلاح الترميمية نظموا وحيد

Conventional techniques for cell expansion and transplantation of autologous cells for tissue engineering purposes can take place in specially equipped ...

Transplantation of Tail Skin to Study Allogeneic CD4 T Cell Responses in Mice

The study of T cell responses and their consequences during allo-antigen recognition requires a model that enables one to distinguish between donor and host T cells, to easily monitor the graft, and to adapt the system in order to answer different immunological questions. Medawar and colleagues established allogeneic tail-skin transplantation in mice in 1955. Since then, the skin transplantation model has been continuously modified and adapted to answer specific questions. The use of tail-skin renders this model easy to score for graft rejection, requires neither extensive preparation nor deep anesthesia, is applicable to animals of all genetic background, discourages ischemic necrosis, and permits chemical and biological intervention. 
In general, both CD4+ and CD8+ allogeneic T cells are responsible for the rejection of allografts since they recognize mismatched major histocompatibility antigens from different mouse strains. Several models have been described for activating allogeneic T cells in skin-transplanted mice. The identification of major histocompatibility complex (MHC) class I and II molecules in different mouse strains including C57BL/6 mice was an important step toward understanding and studying T cell-mediated alloresponses. In the tail-skin transplantation model described here, a three-point mutation (I-Abm12) in the antigen-presenting groove of the MHC-class II (I-Ab) molecule is sufficient to induce strong allogeneic CD4+ T cell activation in C57BL/6 mice. Skin grafts from I-Abm12 mice on C57BL/6 mice are rejected within 12-15 days, while syngeneic grafts are accepted for up to 100 days. The absence of T cells (CD3-/- and Rag2-/- mice) allows skin graft acceptance up to 100 days, which can be overcome by transferring 2 x 104 wild type or transgenic T cells. Adoptively transferred T cells proliferate and produce IFN-&#947; in I-Abm12-transplanted Rag2-/- mice.

Tail-skin transplantation is a powerful model for studying T cell-dependent rejection and tolerance induction during allogeneic immune responses in mice. The advantages of this protocol are minor invasive surgery, and ease of monitoring with no need to sacrifice the recipient mouse.

زرع الجلد الذيل لدراسة خيفي CD4 T الردود الخليوي في الفئران

The study of T cell responses and their consequences during allo-antigen recognition requires a model that enables one to distinguish between donor and ...

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells

The skin is the body&#8217;s largest organ and has many functions. The skin acts as a physical barrier and protector of the body and regulates bodily functions. Biomimetics is the imitation of the models, systems, and elements of nature for the purpose of solving complex human problems1. Skin biomimetics is a useful tool for in vitro disease research and in vivo regenerative medicine. Human induced pluripotent stem cells (iPSCs) have the characteristic of unlimited proliferation and the ability of differentiation to three germ layers. Human iPSCs are generated from various primary cells, such as blood cells, keratinocytes, fibroblasts, and more. Among them, cord blood mononuclear cells (CBMCs) have emerged as an alternative cell source from the perspective of allogeneic regenerative medicine. CBMCs are useful in regenerative medicine because human leukocyte antigen (HLA) typing is essential to the cell banking system. We provide a method for the differentiation of CBMC-iPSCs into keratinocytes and fibroblasts and for generation of a 3D skin organoid. CBMC-iPSC-derived keratinocytes and fibroblasts have characteristics similar to a primary cell line. The 3D skin organoids are generated by overlaying an epidermal layer onto a dermal layer. By transplanting this 3D skin organoid, a humanized mice model is generated. This study shows that a 3D human iPSC-derived skin organoid may be a novel, alternative tool for dermatologic research in vitro and in vivo.

We propose a protocol that shows how to differentiate induced pluripotent stem cell-derived keratinocytes and fibroblasts and generate a 3D skin organoid, using these keratinocytes and fibroblasts. This protocol contains an additional step of generating a humanized mice model. The technique presented here will improve dermatologic research.

جيل أورجانويد الجلد 3D من سلك المستمدة من الدم الناجم عن الخلايا الجذعية Pluripotent

The skin is the body&#8217;s largest organ and has many functions. The skin acts as a physical barrier and protector of the body and regulates bodily ...

Developmental Biology

Xenograft Skin Model to Manipulate Human Immune Responses In Vivo

The human skin xenograft model, in which human donor skin is transplanted onto an immunodeficient mouse host, is an important option for translational research in skin immunology. Murine and human skin differ substantially in anatomy and immune cell composition. Therefore, traditional mouse models have limitations for dermatological research and drug discovery. However, successful xenotransplants are technically challenging and require optimal specimen and mouse graft site preparation for graft and host survival. The present protocol provides an optimized technique for transplanting human skin onto mice and discusses necessary considerations for downstream experimental aims. This report describes the appropriate preparation of a human donor skin sample, assembly of a surgical setup, mouse and surgical site preparation, skin transplantation, and post-surgical monitoring. Adherence to these methods allows for maintenance of xenografts for over 6 weeks post-surgery. The techniques outlined below allow maximum grafting efficiency due to the development of engineering controls, sterile technique, and pre- and post-surgical conditioning. Appropriate performance of the xenograft model results in long-lived human skin graft samples for experimental characterization of human skin and preclinical testing of compounds in vivo.

Xenograft Skin Model to Manipulate Human Immune Responses In Vivo

The present protocol describes how to graft human skin onto non-obese diabetic (NOD)-scid interleukin-2 gamma chain receptor (NSG) mice. A detailed description of the preparation of human skin for transplant, preparation of mice for transplant, transplantation of split-thickness human skin, and post-transplantation recovery procedure are included in the report.

نموذج Xenograft للجلد للتلاعب بالاستجابات المناعية البشرية في الجسم الحي

The human skin xenograft model, in which human donor skin is transplanted onto an immunodeficient mouse host, is an important option for translational ...

A Murine Model of a Burn Wound Reconstructed with an Allogeneic Skin Graft

Trivial superficial wounds heal without complications by primary intention. Deep wounds, such as full thickness burns, heal by secondary intention and require surgical debridement and skin grafting. Successful integration of the donor graft into a recipient wound bed depends on timely recruitment of immune cells, robust angiogenic response and new extracellular matrix formation. The development of novel therapeutic agents, which target some key processes involved in wound healing, are hindered by the lack of reliable preclinical models with optimized objective assessment of wound closure. Here, we describe an inexpensive and reproducible model of experimental full thickness burn wound reconstructed with an allogeneic skin graft. The wound is induced on the dorsum surface of anaesthetized inbred wild type mice from the BALB/C and SKH1-Hrhr backgrounds. The burn is produced using a brass template measuring 10 mm in diameter, which is preheated to 80 &#176;C and delivered at a constant pressure for 20 s. Burn eschar is excised 24 hours after the injury and replaced with a full thickness graft harvested from the tail of a genetically similar donor mouse. No specialized equipment is required for the procedure and surgical techniques are straightforward to follow. The method may be effortlessly implemented and reproduced in most research settings. Certain limitations are associated with the model. Due to technical difficulties, the harvest of thinner split thickness skin grafts is not possible. The surgical method we describe here allows for the reconstruction of burn wounds using full thickness skin grafts. It may be used to carry out preclinical therapeutic testing.

The aim of this study was to develop a murine model of burn wound healing. A thermal burn was induced on the dorsal skin of mice using a preheated brass template. Burned tissue was debrided and overlaid with a skin graft harvested from the tail of a genetically similar donor mouse.

نموذج مورين من جرح حرق أعيد بناؤها مع الكسب غير المشروع الجلد الجيني

Trivial superficial wounds heal without complications by primary intention. Deep wounds, such as full thickness burns, heal by secondary intention and ...

A Technique for Human Skin Grafting in a Mouse Model

Source: Moss, M. I. et al., <a href="https://app.jove.com/v/64040">Xenograft Skin Model to Manipulate Human Immune Responses&#160;In Vivo.</a>&#160;J. Vis. Exp. (2022)
This video demonstrates a method for grafting human skin onto an immunodeficient mouse with a suppressed neutrophil response. The method involves applying human split-thickness skin to the mouse&#39;s graft bed, sealing it, and covering it for recovery. Eventually, the human skin adheres to the mouse&#39;s skin, establishing vascularization and circulation, resulting in a successful skin xenograft.

تقنية لتطعيم جلد الإنسان في نموذج الفأر

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
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