이 작품은 NIH 교부금 R01AI077610에 의해 투자되었다.

모든 절차는 관리 및 국립 보건 연구소 (NIH)의 실험 동물의 사용에 대한 가이드에 따라 실시하고, 존스 홉킨스 대학 동물 관리 및 사용위원회 (JHUACUC)에 의해 승인되었다. 구체적인 절차는 승인 ACUC 프로토콜 MO13M292 및 MO13M370 하에서 수행 하였다. 1. 기증자 피부 수확 <ol><li> (- 마우스 콘을 통해 2 % 1에서 유지 보수, 4 %에서 유도 기화기) 이소 플루 란과 기증자 마우스를 마취. 마취의 깊이를 모니터하기 위해 발가락 핀치 철수 반사를 사용합니다. </li><li> 전기 면도기를 사용하면 동물의 뒤쪽을 면도하고 (예 Betadine) 10 %, 포비돈 요오드로 소독. </li><li> 유륜 결합 조직의 수준에서 무딘 절개와 멸균 가위, 장갑, 및 무균 기술, 목에 엉덩이에서 수확 기증자 다시 피부를 사용. </li><li> 수확 후 자궁 전위에 의해 동물을 안락사피부 이식. </li><li> 현미경, 미세 건 절단술 가위를 사용하여 다시 피부의 결합 조직, 지방 조직 및 panniculus의 카르노 서스를 구분합니다. panniculus의 카르노 서스 피부 경련의 움직임을 담당하는 얇은 투명 근육이다. </li><li> 멸균 악기의 기술을 사용하여, 15 mm X 15 mm 이식 침대로 10 mm × 10-mm에는 후면 피부에서 15 mm X 15 mm 이식을 잘라. </li><li> 얼음에 페트리 접시에 멸균 인산 완충 생리 식염수 (PBS)로 적신 거즈에 이식을 저장합니다. 참고 : 8-10 이식 한 기증자 마우스에서 얻을 수 있습니다. </li></ol> 2.받는 사람 피부 이식 <ol><li> (- 마우스 콘을 통해 2 % 1에서 유지 보수, 4 %에서 유도 기화기) 이소 플루 란과받는 사람 마우스를 마취. 마취의 깊이를 모니터하기 위해 발가락 핀치 철수 반사를 사용합니다. </li><li> 수술 후 통증 완화를 위해 부 프레 놀핀의 0.02 ㎎ / ㎏ BW를 관리 할 수 ​​있습니다. </li><li> 동물의이면 측 면도 여기서 그래프트 WILL 삽입 될 10 % 포비돈 요오드 소독. </li><li> 가위를 사용하여 피부의 X 15 mm 광장 15 mm에 10 mm × 10-mm를 잘라. 결함 크기보다 그래프트 (10 %) 약간 커야한다. 같은 피상적으로 가능한 한 컷. panniculus의 카르노 서스과 혈관을 유지하기 위해주의하십시오. panniculus의 카르노 서스는 이동성과 그것을 피상적 실행 혈관에 의해 기본 밴드 구별 할 수 있습니다. </li><li> 가장자리를 따라 주름을 방지 그래프트 침대에 이식을 배치합니다. </li><li> 모서리에 각 모서리의 중간에 8 봉합을 배치합니다. 각각의 봉합사를 들어, 주변받는 사람의 피부 아래에 침대 이식의 panniculus의 카르노 서스를 통해 다음 이식을 통해 바늘을 통과. </li><li> 마취 마스크를 제거하고 동물이 접착 붕대를 적용하기 전에 마취에서 부분적으로 복구 할 수 있습니다. </li><li> 이식 접혀 거즈와 접착 붕대에받는 사람 마우스를 감싸. 절단, 두 붕대를 결합하여 붕대를 확인접착제의 하나의 부분과 함께 두 흡수 패드를 배치. </li><li> 붕대는 흉부 여행을 제한하고 호흡을하지 않는 것을 보장하기 위해 복구 중에 밀접하게 마우스를 모니터합니다. 호흡 속도가 감소 또는 동물이 얕게 말하다 또는 숨 시작하면, 붕대를 제거합니다. </li></ol> 3. 수술 후 케어 <ol><li> 감염 예방을위한 수술 후 enrofloxacin 5 ㎎ / ㎏을 관리 할 수 ​​있습니다. </li><li> 완전히 마취에서 회복 될 때까지 전자 레인지 가열 패드를 통해 깨끗한 케이지에 이식 된 마우스를 놓습니다. </li><li> 주택 시설에 반환에 수술 전에 1 시간 동안 마우스를 관찰합니다. </li><li> 세븐 일 수술 후, 단계 2.1에서와 같이 마우스를 마취. 붕대의 복부 측면을 통해 만 절단하여 붕대를 제거합니다. </li><li> 관찰하고 가피, 수축, 또는 경도의 증상에 대해 다음 날에 이식을 만져. 존재하는 경우, 이식은 적절한 vasculariza를 달성하지 않을 수 있습니다기와는 기술적 인 실패로 간주되어야한다. </li><li> 거절의 흔적을 매일 모니터링합니다. 이식 조직의 ≥90 %가 괴사 될 때 거부 이식을 고려한다. </li><li> 거부 된 동물을 안락사 및 분석을 수확. </li></ol>

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저자는 공개 아무것도 없어.

토끼와 생쥐에서 다음 인간 연구에서 제 메다에 의해 소개 된 이후, 피부 이식은 동종 면역 반응 6,7의 연구를위한 귀중한 모델이되고있다. 이 논문에서는 상하 다시 피부를 이용한 대규모의 비 혈관 전층 피부 이식 모델을 제시한다. 꼬리 피부 또는 이식 조직 공급원으로서 마우스 귀 피부 사용을 포함하는 다양한 다른 방법은, 8,9까지보고되고있다. 이러한 모델은 단순 기술의 뚜?...

장기 이식은 말기 장기 부전 환자에 대한 선택의 치료이며, 결과는 수술 절차 및 면역 억제 프로토콜의 발전에 현저하게 향상되었습니다. 그러나, 장기간의 면역 억제는 상당한 부작용과 연관 내성을 증진 새로운 전략의 개발은 현대 이식 연구의 목표로 남아있다. 많은 동물 모델은 이식 거부 반응의 메커니즘을 연구하는 면역 억제가 이식 거부 반응을 방지하기위한 장기 공차 1-3 촉진 접근 테스트 이식에 기초 연구 개발되어왔다. 마우스 모델에 의한 진단 및 치료 항체와 잘 정의 된 근친과 유전자 변형 종자의 독점과 광대 한 가용성 면역 학적 연구의 주류가되었다. 피부 이식은 특수 미세 수술 기술을 필요로 할 수없는 간단한 절차입니다쉽게 수술 후 모니터링 될 수있다. 함께 찍은, 마우스 피부 이식은 이식 거부 4,5 동안 항원 전달 세포 인신 매매, 조직 파괴를 포함하여 동종 응답에 관련된 여러 측면을 연구하는 뛰어난 도구가되고있다. 여기서는 마우스 모델을 이용하여 전체 두께 피부 이식을위한 단계별 과정을 보여, 우리는 이식 된 피부의 성공적인 생착에 필요한 중요한 단계를 설명한다.

<table><tbody><tr><td>Straight micro forceps</td><td>Sigma</td><td>F4017</td><td></td></tr><tr><td>Curved micro forceps</td><td>Aesculap</td><td>BD333R</td><td></td></tr><tr><td>Curved Stevens tenotomy scissors</td><td>Aesculap</td><td>BC905R</td><td></td></tr><tr><td>Mayo dissecting scissors</td><td>Sigma</td><td>S3146</td><td></td></tr><tr><td>Micro needle holder</td><td>Aesculap</td><td>BM563R</td><td></td></tr><tr><td>Sterile gauze</td><td>Covidien</td><td>441218</td><td></td></tr><tr><td>6-0 Nylon suture</td><td>MWI</td><td>31849</td><td></td></tr><tr><td>Plastic Strips Band&amp;#45;Aid</td><td>Johnson &amp;amp; Johnson</td><td></td><td>Obtained from pharmacy</td></tr><tr><td>10 cm Petri dish</td><td>Fisherbrand</td><td>FB0875712</td><td></td></tr><tr><td>PBS</td><td>Quality Biological</td><td>119069131</td><td></td></tr><tr><td>Buprenorphine</td><td></td><td></td><td>DEA Number required; Obtained from hospital pharmacy</td></tr><tr><td>Enrofloxacin</td><td>Bayer Health Care</td><td>186599</td><td></td></tr></tbody></table>

murine full-thickness skin transplantation

쥐의 전체 두께 피부 이식은 동종 반응과 이식 거부 반응을 연구하는 잘 설립 생체 내 모델입니다. 인간에게 제한된 응용 프로그램에도 불구하고, 마우스의 피부 이식 널리 이식 연구에 이용되고있다. 절차 배우고 수행하는 것이 용이하고, 섬세한 미세 수술 기술도 광범위한 훈련을 필요로하지 않는다. 또한,이 모델의 이식 거부는 매우 재현 면역 반응에서 발생하고 쉽게 직접 검사 및 촉진에 의해 모니터링된다. 또한, 기증자 일치 또는 타사 피부 이식 2 차 피부 이식은 다른 기증자 별 허용 오차를 평가하는 복잡하지 않은 방법으로 더 복잡한 이식 모델에서 수행 할 수 있습니다. 합병증은 낮으며 시술 후 마취 과다 복용 또는 호흡 곤란으로 제한 일반적이다. 이식 실패는 한편, GR의 불량한 제조의 결과로서 통상적으로 발생그래프트 침대에서 후미, 잘못된 위치, 또는 붕대의 부적절한 배치. 이 글에서, 우리는 생쥐에서 전층 피부 이식을위한 프로토콜을 제시하고 성공적인 수속에 필요한 중요한 단계를 설명합니다.

받는 사람 마우스의 붕대의 위치는 절차의 중요한 단계입니다. 피부 이식은 어깨, 고관절, 척추 (그림 1) 사이에,받는 사람 트렁크에 위치한다. 붕대 접혀 거즈와 두 개의 플라스틱 접착 붕대의 조합으로 이루어집니다. 받는 사람 마우스는 붕대의 중심에 거즈를 통해 아래로 이식으로 배치됩니다. 두 곡선의 마이크로 핀셋을 사용하여, 붕대의 하단부는 제 당겨...

Watch this Scientific Journal Video about Murine Full-thickness Skin Transplantation at JoVE.com

Murine Full-thickness Skin Transplantation

쥐의 전체 두께 피부 이식은 동종 설정에 거부 반응을 연구하는 잘 확립 된 모델이다. &gt; C57BL / 6 전층 피부 이식 - 여기, 우리는 BALB / C를 수행에 관련된 각 단계의 튜토리얼을 제공합니다.

쥐의 전체 두께 피부 이식

Murine full-thickness skin transplantation is a well-established in vivo model to study alloimmune response and graft rejection. Despite its limited ...

murine-full-thickness-skin-transplantation

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JoVE Journal

Immunology and Infection

20.0K 조회수.  Johns Hopkins University School of Medicine.  쥐의 전체 두께 피부 이식은 동종 설정에 거부 반응을 연구하는 잘 확립 된 모델이다. > C57BL / 6 전층 피부 이식 - 여기, 우리는 BALB / C를 수행에 관련된 각 단계의 튜토리얼을 제공합니다. 

비디오: Murine Full-thickness Skin Transplantation

The Three-Dimensional Human Skin Reconstruct Model: a Tool to Study Normal  Skin and Melanoma Progression

Most in vitro studies in experimental skin biology have been done in 2-dimensional (2D) monocultures, while accumulating evidence suggests that cells behave differently when they are grown within a 3D extra-cellular matrix and also interact with other cells (1-5). Mouse models have been broadly utilized to study tissue morphogenesis in vivo. However mouse and human skin have significant differences in cellular architecture and physiology, which makes it difficult to extrapolate mouse studies to humans. Since melanocytes in mouse skin are mostly localized in hair follicles, they have distinct biological properties from those of humans, which locate primarily at the basal layer of the epidermis. The recent development of 3D human skin reconstruct models has enabled the field to investigate cell-matrix and cell-cell interactions between different cell types. The reconstructs consist of a "dermis" with fibroblasts embedded in a collagen I matrix, an "epidermis", which is comprised of stratified, differentiated keratinocytes and a functional basement membrane, which separates epidermis from dermis. Collagen provides scaffolding, nutrient delivery, and potential for cell-to-cell interaction. The 3D skin models incorporating melanocytic cells recapitulate natural features of melanocyte homeostasis and melanoma progression in human skin. As in vivo, melanocytes in reconstructed skin are localized at the basement membrane interspersed with basal layer keratinocytes. Melanoma cells exhibit the same characteristics reflecting the original tumor stage (RGP, VGP and metastatic melanoma cells) in vivo. Recently, dermal stem cells have been identified in the human dermis (6). These multi-potent stem cells can migrate to the epidermis and differentiate to melanocytes.

In this report, we describe the three-dimensional skin reconstruct model which mimics human skin in architecture and composition. Melanocyte physiology, melanoma progression and the fate of dermal stem cells have been investigated using the skin reconstruct model. The model is also useful as a preclinical tool for drug assessment.

3 차원의 인간 피부 모델을 재구성 : 도구는 일반 피부와 흑색증의 진행을 공부하기

Most in vitro studies in experimental skin biology have been done in 2-dimensional (2D) monocultures, while accumulating evidence suggests that cells ...

연구

생체공학

Murine Skin Transplantation

As one of the most stringent and least technically challenging models, skin transplantation is a standard method to assay host T cell responses to MHC-disparate donor antigens. The aim of this video-article is to provide the viewer with a step-by-step visual demonstration of skin transplantation using the mouse model. The protocol is divided into 5 main components: 1) harvesting donor skin; 2) preparing recipient for transplant; 3) skin transplant; 4) bandage removal and monitoring graft rejection; 5) helpful hints. Once proficient, the procedure itself should take &lt;10 min to perform.

Allogeneic skin transplantation is a standard model to assay host T cell responses to MHC-disparate donor antigens. This video-article provides a visual tutorial of each step involved in performing a BALB/c--&gt;C57BL/6 skin transplant.

Murine 피부 이식

As one of the most stringent and least technically challenging models, skin transplantation is a standard method to assay host T cell responses to ...

생물학

A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo

Insufficient vascularization is considered to be one of the main factors limiting the clinical success of tissue-engineered constructs. In order to evaluate new strategies that aim at improving vascularization, reliable methods are required to make the in-growth of new blood vessels into bio-artificial scaffolds visible and quantify the results. Over the past couple of years, our group has introduced a full skin defect model that enables the direct visualization of blood vessels by transillumination and provides the possibility of quantification through digital segmentation. In this model, one surgically creates full skin defects in the back of mice and replaces them with the material tested. Molecules or cells of interest can also be incorporated in such materials to study their potential effect. After an observation time of one&#8217;s own choice, materials are explanted for evaluation. Bilateral wounds provide the possibility of making internal comparisons that minimize artifacts among individuals as well as of decreasing the number of animals needed for the study. In comparison to other approaches, our method offers a simple, reliable and cost effective analysis. We have implemented this model as a routine tool to perform high-resolution screening when testing vascularization of different biomaterials and bio-activation approaches.

A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo

Vascularization is key to approaches in successful tissue engineering. Therefore, reliable technologies are required to evaluate the development of vascular networks in tissue-constructs. Here we present a simple and cost-effective method to visualize and quantify vascularization in vivo.

전체 피부 결함 모델은 생체의 혈관 신생을 평가하기<em&gt; 생체</em

Insufficient vascularization is considered to be one of the main factors limiting the clinical success of tissue-engineered constructs. In order to ...

Murine Model of Wound Healing

Wound healing and repair are the most complex biological processes that occur in human life. After injury, multiple biological pathways become activated. Impaired wound healing, which occurs in diabetic patients for example, can lead to severe unfavorable outcomes such as amputation. There is, therefore, an increasing impetus to develop novel agents that promote wound repair. The testing of these has been limited to large animal models such as swine, which are often impractical. Mice represent the ideal preclinical model, as they are economical and amenable to genetic manipulation, which allows for mechanistic investigation. However, wound healing in a mouse is fundamentally different to that of humans as it primarily occurs via contraction. Our murine model overcomes this by incorporating a splint around the wound. By splinting the wound, the repair process is then dependent on epithelialization, cellular proliferation and angiogenesis, which closely mirror the biological processes of human wound healing. Whilst requiring consistency and care, this murine model does not involve complicated surgical techniques and allows for the robust testing of promising agents that may, for example, promote angiogenesis or inhibit inflammation. Furthermore, each mouse acts as its own control as two wounds are prepared, enabling the application of both the test compound and the vehicle control on the same animal. In conclusion, we demonstrate a practical, easy-to-learn, and robust model of wound healing, which is comparable to that of humans.

A murine model of cutaneous wound healing that can be used to assess therapeutic compounds in physiological and pathophysiological settings.

상처 치유의 생쥐 모델

Wound healing and repair are the most complex biological processes that occur in human life. After injury, multiple biological pathways become activated. ...

의학

Minced Tissue in Compressed Collagen: A Cell-containing Biotransplant for Single-staged Reconstructive Repair

Conventional techniques for cell expansion and transplantation of autologous cells for tissue engineering purposes can take place in specially equipped human cell culture facilities. These methods include isolation of cells in single cell suspension and several laborious and time-consuming events before transplantation back to the patient. Previous studies suggest that the body itself could be used as a bioreactor for cell expansion and regeneration of tissue in order to minimize ex vivo manipulations of tissues and cells before transplanting to the patient. The aim of this study was to demonstrate a method for tissue harvesting, isolation of continuous epithelium, mincing of the epithelium into small pieces and incorporating them into a three-layered biomaterial. The three-layered biomaterial then served as a delivery vehicle, to allow surgical handling, exchange of nutrition across the transplant, and a controlled degradation. The biomaterial consisted of two outer layers of collagen and a core of a mechanically stable and slowly degradable polymer. The minced epithelium was incorporated into one of the collagen layers before transplantation. By mincing the epithelial tissue into small pieces, the pieces could be spread and thereby the propagation of cells was stimulated. After the initial take of the transplants, cell expansion and reorganization would take place and extracellular matrix mature to allow ingrowth of capillaries and nerves and further maturation of the extracellular matrix. The technique minimizes ex vivo manipulations and allow cell harvesting, preparation of autograft, and transplantation to the patient as a simple one-stage intervention. In the future, tissue expansion could be initiated around a 3D mold inside the body itself, according to the specific needs of the patient. Additionally, the technique could be performed in an ordinary surgical setting without the need for sophisticated cell culturing facilities.

Tissue engineering often includes in vitro expansion in order to create autografts for tissue regeneration. In this study a method for tissue expansion, regeneration, and reconstruction in vivo was developed in order to minimize the processing of cells and biological materials outside the body.

압축 된 콜라겐에 다진 조직 : 단일 무대 재건 복구를위한 세포 함유 Biotransplant

Conventional techniques for cell expansion and transplantation of autologous cells for tissue engineering purposes can take place in specially equipped ...

Transplantation of Tail Skin to Study Allogeneic CD4 T Cell Responses in Mice

The study of T cell responses and their consequences during allo-antigen recognition requires a model that enables one to distinguish between donor and host T cells, to easily monitor the graft, and to adapt the system in order to answer different immunological questions. Medawar and colleagues established allogeneic tail-skin transplantation in mice in 1955. Since then, the skin transplantation model has been continuously modified and adapted to answer specific questions. The use of tail-skin renders this model easy to score for graft rejection, requires neither extensive preparation nor deep anesthesia, is applicable to animals of all genetic background, discourages ischemic necrosis, and permits chemical and biological intervention. 
In general, both CD4+ and CD8+ allogeneic T cells are responsible for the rejection of allografts since they recognize mismatched major histocompatibility antigens from different mouse strains. Several models have been described for activating allogeneic T cells in skin-transplanted mice. The identification of major histocompatibility complex (MHC) class I and II molecules in different mouse strains including C57BL/6 mice was an important step toward understanding and studying T cell-mediated alloresponses. In the tail-skin transplantation model described here, a three-point mutation (I-Abm12) in the antigen-presenting groove of the MHC-class II (I-Ab) molecule is sufficient to induce strong allogeneic CD4+ T cell activation in C57BL/6 mice. Skin grafts from I-Abm12 mice on C57BL/6 mice are rejected within 12-15 days, while syngeneic grafts are accepted for up to 100 days. The absence of T cells (CD3-/- and Rag2-/- mice) allows skin graft acceptance up to 100 days, which can be overcome by transferring 2 x 104 wild type or transgenic T cells. Adoptively transferred T cells proliferate and produce IFN-&#947; in I-Abm12-transplanted Rag2-/- mice.

Tail-skin transplantation is a powerful model for studying T cell-dependent rejection and tolerance induction during allogeneic immune responses in mice. The advantages of this protocol are minor invasive surgery, and ease of monitoring with no need to sacrifice the recipient mouse.

마우스에 동종 CD4 T 세포 반응을 연구하기 위해 테일 피부 이식

The study of T cell responses and their consequences during allo-antigen recognition requires a model that enables one to distinguish between donor and ...

면역학 및 감염병학

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells

The skin is the body&#8217;s largest organ and has many functions. The skin acts as a physical barrier and protector of the body and regulates bodily functions. Biomimetics is the imitation of the models, systems, and elements of nature for the purpose of solving complex human problems1. Skin biomimetics is a useful tool for in vitro disease research and in vivo regenerative medicine. Human induced pluripotent stem cells (iPSCs) have the characteristic of unlimited proliferation and the ability of differentiation to three germ layers. Human iPSCs are generated from various primary cells, such as blood cells, keratinocytes, fibroblasts, and more. Among them, cord blood mononuclear cells (CBMCs) have emerged as an alternative cell source from the perspective of allogeneic regenerative medicine. CBMCs are useful in regenerative medicine because human leukocyte antigen (HLA) typing is essential to the cell banking system. We provide a method for the differentiation of CBMC-iPSCs into keratinocytes and fibroblasts and for generation of a 3D skin organoid. CBMC-iPSC-derived keratinocytes and fibroblasts have characteristics similar to a primary cell line. The 3D skin organoids are generated by overlaying an epidermal layer onto a dermal layer. By transplanting this 3D skin organoid, a humanized mice model is generated. This study shows that a 3D human iPSC-derived skin organoid may be a novel, alternative tool for dermatologic research in vitro and in vivo.

We propose a protocol that shows how to differentiate induced pluripotent stem cell-derived keratinocytes and fibroblasts and generate a 3D skin organoid, using these keratinocytes and fibroblasts. This protocol contains an additional step of generating a humanized mice model. The technique presented here will improve dermatologic research.

코드 혈액 파생에서 3D 피부 Organoid의 생성 유도 만능 줄기 세포

The skin is the body&#8217;s largest organ and has many functions. The skin acts as a physical barrier and protector of the body and regulates bodily ...

발생학

Xenograft Skin Model to Manipulate Human Immune Responses In Vivo

The human skin xenograft model, in which human donor skin is transplanted onto an immunodeficient mouse host, is an important option for translational research in skin immunology. Murine and human skin differ substantially in anatomy and immune cell composition. Therefore, traditional mouse models have limitations for dermatological research and drug discovery. However, successful xenotransplants are technically challenging and require optimal specimen and mouse graft site preparation for graft and host survival. The present protocol provides an optimized technique for transplanting human skin onto mice and discusses necessary considerations for downstream experimental aims. This report describes the appropriate preparation of a human donor skin sample, assembly of a surgical setup, mouse and surgical site preparation, skin transplantation, and post-surgical monitoring. Adherence to these methods allows for maintenance of xenografts for over 6 weeks post-surgery. The techniques outlined below allow maximum grafting efficiency due to the development of engineering controls, sterile technique, and pre- and post-surgical conditioning. Appropriate performance of the xenograft model results in long-lived human skin graft samples for experimental characterization of human skin and preclinical testing of compounds in vivo.

Xenograft Skin Model to Manipulate Human Immune Responses In Vivo

The present protocol describes how to graft human skin onto non-obese diabetic (NOD)-scid interleukin-2 gamma chain receptor (NSG) mice. A detailed description of the preparation of human skin for transplant, preparation of mice for transplant, transplantation of split-thickness human skin, and post-transplantation recovery procedure are included in the report.

생체 내에서 인간 면역 반응을 조작하기 위한 이종이식 피부 모델

The human skin xenograft model, in which human donor skin is transplanted onto an immunodeficient mouse host, is an important option for translational ...

A Murine Model of a Burn Wound Reconstructed with an Allogeneic Skin Graft

Trivial superficial wounds heal without complications by primary intention. Deep wounds, such as full thickness burns, heal by secondary intention and require surgical debridement and skin grafting. Successful integration of the donor graft into a recipient wound bed depends on timely recruitment of immune cells, robust angiogenic response and new extracellular matrix formation. The development of novel therapeutic agents, which target some key processes involved in wound healing, are hindered by the lack of reliable preclinical models with optimized objective assessment of wound closure. Here, we describe an inexpensive and reproducible model of experimental full thickness burn wound reconstructed with an allogeneic skin graft. The wound is induced on the dorsum surface of anaesthetized inbred wild type mice from the BALB/C and SKH1-Hrhr backgrounds. The burn is produced using a brass template measuring 10 mm in diameter, which is preheated to 80 &#176;C and delivered at a constant pressure for 20 s. Burn eschar is excised 24 hours after the injury and replaced with a full thickness graft harvested from the tail of a genetically similar donor mouse. No specialized equipment is required for the procedure and surgical techniques are straightforward to follow. The method may be effortlessly implemented and reproduced in most research settings. Certain limitations are associated with the model. Due to technical difficulties, the harvest of thinner split thickness skin grafts is not possible. The surgical method we describe here allows for the reconstruction of burn wounds using full thickness skin grafts. It may be used to carry out preclinical therapeutic testing.

The aim of this study was to develop a murine model of burn wound healing. A thermal burn was induced on the dorsal skin of mice using a preheated brass template. Burned tissue was debrided and overlaid with a skin graft harvested from the tail of a genetically similar donor mouse.

Trivial superficial wounds heal without complications by primary intention. Deep wounds, such as full thickness burns, heal by secondary intention and ...

A Technique for Human Skin Grafting in a Mouse Model

Source: Moss, M. I. et al., <a href="https://app.jove.com/v/64040">Xenograft Skin Model to Manipulate Human Immune Responses&#160;In Vivo.</a>&#160;J. Vis. Exp. (2022)
This video demonstrates a method for grafting human skin onto an immunodeficient mouse with a suppressed neutrophil response. The method involves applying human split-thickness skin to the mouse&#39;s graft bed, sealing it, and covering it for recovery. Eventually, the human skin adheres to the mouse&#39;s skin, establishing vascularization and circulation, resulting in a successful skin xenograft.

마우스 모델에서 인간 피부 이식을 위한 기술

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. ...