Ward C. Tucker

Role of synaptotagmin in Ca2+-triggered exocytosis.

Visualization of synaptotagmin I oligomers assembled onto lipid monolayers.

Identification of synaptotagmin effectors via acute inhibition of secretion from cracked PC12 cells.

PIP2 increases the speed of response of synaptotagmin and steers its membrane-penetration activity toward the plasma membrane.

ENaC subunit-subunit interactions and inhibition by syntaxin 1A.

Reconstitution of Ca2+-regulated membrane fusion by synaptotagmin and SNAREs.

Observation of calcium-dependent unidirectional rotational motion in recombinant photosynthetic F1-ATPase molecules.

Molecular regulation of membrane resealing in 3T3 fibroblasts.

SNARE-driven, 25-millisecond vesicle fusion in vitro.

Synaptotagmin isoforms couple distinct ranges of Ca2+, Ba2+, and Sr2+ concentration to SNARE-mediated membrane fusion.

Ca(2+)-synaptotagmin directly regulates t-SNARE function during reconstituted membrane fusion.

Mutations within the C-terminus of the gamma subunit of the photosynthetic F1-ATPase activate MgATP hydrolysis and attenuate the stimulatory oxyanion effect.

Detection of six serotypes of botulinum neurotoxin using fluorogenic reporters.

In vitro detection and quantification of botulinum neurotoxin type e activity in avian blood.

Detection of botulinum neurotoxin serotype A, B, and F proteolytic activity in complex matrices with picomolar to femtomolar sensitivity.