Xianyong Liu
National Animal Protozoa Laboratory,
College of Veterinary Medicine,
National Animal Protozoa Laboratory, College of Veterinary Medicine
China Agricultural University
Xianyong Liu is an Associate Professor in the Veterinary Parasitology Department, College of Veterinary Medicine of China Agricultural University (CAU) in Beijing. He received his undergraduate degree and a Ph.D. from this university.
During Dr. Xianyong’s training he optimized the protocol for the development of transient and stable transfection of Eimeria tenella, a protozoa causing cecal coccidiosis in chicken. As a post-doctoral fellow (2012 to 2013) in Prof James Lok’s lab at Pathobiology Department, University of Pennsylvania, US, he tried the TALEN tool in Strongyloides stecoralis.
Dr. Xianyong was enrolled as a lecturer at CAU. His research program encompasses eimerian parasites of chicken and rabbits to develop molecular tools for the genetic manipulation of these parasites, as well as to develop vaccines based on the genetically modified eimerian parasites.
Transient transfection of Eimeria tenella using yellow or red fluorescent protein as a marker.
Molecular and biochemical parasitology Jun, 2007 | Pubmed ID: 17399806
Restriction enzyme-mediated transfection improved transfection efficiency in vitro in Apicomplexan parasite Eimeria tenella.
Molecular and biochemical parasitology Sep, 2008 | Pubmed ID: 18606196
Stable transfection of Eimeria tenella: constitutive expression of the YFP-YFP molecule throughout the life cycle.
International journal for parasitology Jan, 2009 | Pubmed ID: 18718473
Dynamic development of parasitophorous vacuole of Eimeria tenella transfected with the yellow fluorescent protein gene fused to different signal sequences from apicomplexan parasites.
Parasitology research Jan, 2009 | Pubmed ID: 18815811
Transfection of Eimeria and Toxoplasma using heterologous regulatory sequences.
International journal for parasitology Sep, 2009 | Pubmed ID: 19379753
Co-expression of reporter genes in the widespread pathogen Eimeria tenella using a double-cassette expression vector strategy.
International journal for parasitology Jul, 2011 | Pubmed ID: 21550346
Transgenic Eimeria tenella expressing enhanced yellow fluorescent protein targeted to different cellular compartments stimulated dichotomic immune responses in chickens.
Journal of immunology (Baltimore, Md. : 1950) Oct, 2011 | Pubmed ID: 21876035
Large-scale survey of the prevalence of Eimeria infections in domestic rabbits in China.
Parasitology research Apr, 2012 | Pubmed ID: 21972072
High transfection efficiency of porcine peripheral blood T cells via nucleofection.
Veterinary immunology and immunopathology Dec, 2011 | Pubmed ID: 22055481
Molecular cloning and characterization of the full-length cDNA encoding the porcine CD28.
Veterinary immunology and immunopathology May, 2012 | Pubmed ID: 22510524
piggyBac transposon-mediated transgenesis in the apicomplexan parasite Eimeria tenella.
PloS one , 2012 | Pubmed ID: 22768223
Plasmid DNA could be delivered into Eimeria maxima unsporulated oocyst with gene gun system.
Acta veterinaria Hungarica Dec, 2012 | Pubmed ID: 23160025
Development of transgenic lines of Eimeria tenella expressing M2e-enhanced yellow fluorescent protein (M2e-EYFP).
Veterinary parasitology Mar, 2013 | Pubmed ID: 23298569
Expression of Toxoplasma gondii dense granule protein7 (GRA7) in Eimeria tenella.
Parasitology research May, 2013 | Pubmed ID: 23371499
Molecular cloning and protein characterization of swine 4-1BB.
Veterinary immunology and immunopathology May, 2013 | Pubmed ID: 23453329
Interferon-γ enzyme-linked immunosorbent spot assay as a tool to study T cell responses to Eimeria tenella infection in chickens.
Poultry science Jul, 2013 | Pubmed ID: 23776262
An Eimeria vaccine candidate based on Eimeria tenella immune mapped protein 1 and the TLR-5 agonist Salmonella typhimurium FliC flagellin.
Biochemical and biophysical research communications Oct, 2013 | Pubmed ID: 24076159
CDR3 analysis of TCR Vβ repertoire of CD8⁺ T cells from chickens infected with Eimeria maxima.
Experimental parasitology Aug, 2014 | Pubmed ID: 24801021
Molecular analysis of single oocyst of Eimeria by whole genome amplification (WGA) based nested PCR.
Experimental parasitology Sep, 2014 | Pubmed ID: 24996066
Ethanol and isopropanol trigger rapid egress of intracellular Eimeria tenella sporozoites.
Parasitology research Feb, 2015 | Pubmed ID: 25407127
Protective immunity against Eimeria tenella infection in chickens induced by immunization with a recombinant C-terminal derivative of EtIMP1.
Veterinary immunology and immunopathology Dec, 2014 | Pubmed ID: 25464823
Exploring early and late Toxoplasma gondii strain RH infection by two-dimensional immunoblots of chicken immunoglobulin G and M profiles.
PloS one , 2015 | Pubmed ID: 25803039
Immunogenicity and protective efficacy of an Eimeria vaccine candidate based on Eimeria tenella immune mapped protein 1 and chicken CD40 ligand.
Veterinary parasitology May, 2015 | Pubmed ID: 25840621
Transgenic Eimeria mitis expressing chicken interleukin 2 stimulated higher cellular immune response in chickens compared with the wild-type parasites.
Frontiers in microbiology , 2015 | Pubmed ID: 26082759
Nitric oxide stimulates early egress of Toxoplasma gondii tachyzoites from Human foreskin fibroblast cells.
Parasites & vectors Aug, 2015 | Pubmed ID: 26264067
Interferon-Gamma Release Assay: An Effective Tool to Detect Early Toxoplasma gondii Infection in Mice.
PloS one , 2015 | Pubmed ID: 26378802
Immunoproteomic technology offers an extraordinary diagnostic approach for Toxoplasma gondii infection.
Journal of microbiological methods Dec, 2015 | Pubmed ID: 26415530
From the Macro to the Micro: Gel Mapping to Differentiate between Sporozoites of Two Immunologically Distinct Strains of Eimeria maxima (Strains M6 and Guelph).
PloS one , 2015 | Pubmed ID: 26641262
Chicken IgY Fc expressed by Eimeria mitis enhances the immunogenicity of E. mitis.
Parasites & vectors Mar, 2016 | Pubmed ID: 27000834
"Self-cleaving" 2A peptide from porcine teschovirus-1 mediates cleavage of dual fluorescent proteins in transgenic Eimeria tenella.
Veterinary research 06, 2016 | Pubmed ID: 27352927
Survey of coccidial infection of rabbits in Sichuan Province, Southwest China.
SpringerPlus , 2016 | Pubmed ID: 27386319
Transgenic Eimeria tenella as a vaccine vehicle: expressing TgSAG1 elicits protective immunity against Toxoplasma gondii infections in chickens and mice.
Scientific reports 07, 2016 | Pubmed ID: 27387302
Calcium-dependent microneme protein discharge and in vitro egress of Eimeria tenella sporozoites.
Experimental parasitology Nov, 2016 | Pubmed ID: 27680997
High pathogenicity and strong immunogenicity of a Chinese isolate of Eimeria magna Pérard, 1925.
Parasitology international Jun, 2017 | Pubmed ID: 28132801
Transgenic Pérard, 1925 Displays Similar Parasitological Properties to the Wild-type Strain and Induces an Exogenous Protein-Specific Immune Response in Rabbits ( L.).
Frontiers in immunology , 2017 | Pubmed ID: 28167939
Mucosal IgA and IFN-γ CD8 T cell immunity are important in the efficacy of live Salmonella enteria serovar Choleraesuis vaccines.
Scientific reports 04, 2017 | Pubmed ID: 28406162
A new species of Eimeria (Apicomplexa: Eimeriidae) from Californian rabbits in Hebei Province, China.
Parasitology international Oct, 2017 | Pubmed ID: 28663008
Increased Neutralizing Antibody Production and Interferon-γ Secretion in Response to Porcine Reproductive and Respiratory Syndrome Virus Immunization in Genetically Modified Pigs.
Frontiers in immunology , 2017 | Pubmed ID: 28955336
Improved Cytotoxic T Lymphocyte Responses to Vaccination with Porcine Reproductive and Respiratory Syndrome Virus in 4-1BB Transgenic Pigs.
Frontiers in immunology , 2017 | Pubmed ID: 29326720
A Novel Vaccine Delivery Model of the Apicomplexan Expressing Antigen Protects Chickens against Infection of the Two Parasites.
Frontiers in immunology , 2017 | Pubmed ID: 29375584
An optimized DNA extraction method for molecular identification of coccidian species.
Parasitology research Mar, 2018 | Pubmed ID: 29396674
Influence of Eimeria falciformis Infection on Gut Microbiota and Metabolic Pathways in Mice.
Infection and immunity 05, 2018 | Pubmed ID: 29440368
Transgenic Eimeria tenella Expressing Profilin of Eimeria maxima Elicits Enhanced Protective Immunity and Alters Gut Microbiome of Chickens.
Infection and immunity 09, 2018 | Pubmed ID: 29967093
Eimeria tenella infection perturbs the chicken gut microbiota from the onset of oocyst shedding.
Veterinary parasitology Jul, 2018 | Pubmed ID: 30105975
Comparative transcriptome analysis of Eimeria maxima (Apicomplexa: Eimeriidae) suggests DNA replication activities correlating with its fecundity.
BMC genomics Sep, 2018 | Pubmed ID: 30249186
Stable transfection of Eimeria necatrix through nucleofection of second generation merozoites.
Molecular and biochemical parasitology 03, 2019 | Pubmed ID: 30658178
Selection and identification of a precocious line of Eimeria intestinalis with enlarged oocysts and deletion of one generation of schizogony.
Parasitology research Mar, 2019 | Pubmed ID: 30694415
Inflammasome activation restrains the intracellular Neospora caninum proliferation in bovine macrophages.
Veterinary parasitology Apr, 2019 | Pubmed ID: 30981301
Transcriptional profiling of innate immune responses in sheep PBMCs induced by Haemonchus contortus soluble extracts.
Parasites & vectors Apr, 2019 | Pubmed ID: 31023355
Identification of candidate antigens by 2-DE Immunoblotting for diagnosis of Toxoplasma gondii infection in chickens and rabbits.
Experimental parasitology Sep, 2019 | Pubmed ID: 31299265
Co-immunization with two recombinant Eimeria tenella lines expressing immunoprotective antigens of E. maxima elicits enhanced protection against E. maxima infection.
Parasites & vectors Jul, 2019 | Pubmed ID: 31300007
Selection and characterization of a precocious line of Eimeria media.
Parasitology research Oct, 2019 | Pubmed ID: 31407118
Immune protection provided by a precocious line trivalent vaccine against rabbit Eimeria.
Veterinary parasitology Nov, 2019 | Pubmed ID: 31639571
