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Nucleofection and In Vivo Propagation of Chicken Eimeria Parasites

DOI :

10.3791/60552-v

February 14th, 2020

February 14th, 2020

6,575 Views

1National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, 2School of Life Sciences, Peking University

Here, we provided a method to achieve stable transfection of chicken Eimeria parasites by nucleofecting sporozoites or second-generation merozoites. Genetically modified eimerian parasites expressing heterologous antigenic genes could be used as vaccine delivery vehicles.

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Nucleofection

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