1Department of Biomedical Engineering, School of Medicine, Tsinghua University, 2Beijing CytoNiche Biotechnology Co. Ltd.
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Summary
Here, we present a protocol to achieve the large-scale manufacturing of adherent cells through a fully closed system based on GMP-grade dissolvable microcarriers. The cultivation of human mesenchymal stem cells, HEK293T cells, and Vero cells was validated and met both quantity demands and quality criteria for the cell and gene therapy industry.
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