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Cancer Research

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Methods Article

Development and Optimization of A Human Hepatocellular Carcinoma Patient-Derived Organoid Model for Potential Target Identification and Drug Discovery

1.3K Views

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07:25 min

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August 18th, 2023

DOI :

10.3791/65785-v

August 18th, 2023

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Cheng-Yang Zhang*¹^,², Xiao-Feng Zhang*³, Jun Yuan*¹^,², Yuan-Feng Gong⁴, Hui Tang⁴, Wan-Yu Guo¹^,², Tong-Yan Li¹^,², Cai-Wen Li¹^,², Yun-Qiang Tang⁴, Ning-Fang Ma¹^,², Ming Liu¹^,²

¹Affiliated Cancer Hospital and Institute of Guangzhou Medical University, ²Guangzhou Municipal and Guangdong Provincial Key Laboratory of Protein Modification and Degradation, Center for Cancer Research and Translational Medicine, School of Basic Medical Sciences, Guangzhou Medical University, ³Department of Pediatric Surgery, Guangzhou Institute of Pediatrics, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, ⁴Department of Hepatobiliary Surgery, Affiliated Cancer Hospital, Guangzhou Medical University

Transcript

Our lab aims to investigate the molecular pathogenesis of liver cancer and identify novel therapeutic targets for drug development. We would like to answer how cancer initiates, metastasis, and progress to drug tolerance utilizing novel disease models such as patient derived organoids. The current experimental challenges are in two aspects.

First, there is a lack of complete set of protocols for constructing hepatocellular carcinoma organoids for the whole process. Second, the size of cultured HCC organoids is too small for subsequent experimental processing. In this protocol, we provide the details of the optimized organoids culture and subsequent transfer and freezing procedures, as well as the precautions to be taken during all the procedures.

We optimized the composition of the organoid medium for organoids with close to the original tissue characteristics. We may monitor the tumor evolution using the patient-derived organoids and try to identify novel molecular and therapeutic targets for HCC treatment.

Summary

We provide a comprehensive overview and refinement of existing protocols for hepatocellular carcinoma (HCC) organoid formation, encompassing all stages of organoid cultivation. This system serves as a valuable model for the identification of potential therapeutic targets and the assessment of drug candidate effectiveness.

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Chapters in this video

0:00

Introduction

1:26

Hepatocellular Carcinoma Tissue Dissociation for Organoid Culture