Inoculating and Observing Arbuscular Mycorrhizal Cultures on Superabsorbent Polymer-Based Autotrophic Systems

Basically, all fungi are ancient and essential plant food symbionts, and they enhance the nutrient uptake of the host and are also key players in cell health. While cell microorganisms are difficult to study, our protocol allows for real time monitoring, digital observation, and efficient establishment of single spoke cultures. Currently, AM fungi are propagated in pot culture, in greenhouse condition, or in vitro in using Ri T-DNA transform root.

Both technology have significantly contributed to the knowledge of those AM fungi in the last decade, but they have specific limitations. Assessment of successful establishment of the fungal partner relies on the presence of arbuscular in the roots or spore in the soil. In vitro propagation allows lives, more monitoring, but requires transform routes and sterile condition limiting its applicability to many AM fungal species.

The pot culture or in vitro culture techniques used to study AMF have limitations in terms of live observation and successful propagation. To overcome these limitations, we have developed this super absorbent, polymer-based autotroph system, SAP-AS, which is a simple and inexpensive technique that combines the advantages of in vitro culture and pot culture. Our laboratory will focus on improving the establishment of new AM fungal species in single spore cultures and also studying the interaction we oversaw in microorganisms.

This technique will allow us to study the competitive dynamics between different AM fungal species, high fault propagation mechanism, and nutrient uptake processes.