zhongnan hospital of Wuhan University

9 ARTICLES PUBLISHED IN JoVE

Biology

A Technique for Serial Collection of Cerebrospinal Fluid from the Cisterna Magna in Mouse

¹Department of Pathology, Columbia University

Transgenic (Tg) mouse models of AD provide an excellent opportunity to investigate how and why Aβ or tau levels in CSF change as the disease progresses in human patients. Here, we demonstrate a refined cisterna magna puncture technique for serial CSF sampling from the mouse.

JoVE Journal

Small and Wide Angle X-Ray Scattering Studies of Biological Macromolecules in Solution

Li Liu¹, Lauren Boldon¹, Melissa Urquhart¹, Xiangyu Wang¹

¹Department of Mechanical, Aerospace, and Nuclear Engineering, Rensselaer Polytechnic Institute

The demonstration of the small and wide angle X-ray scattering (SWAXS) procedure has become instrumental in the study of biological macromolecules. Through the use of the instrumentation and procedures of specific angle methods and preparation, the experimental data from the SWAXS displays the atomic and nano-scale characterization of macromolecules.

Biology

Determination of Sialic Acids in Liver and Milk Samples of Wild-type and CMAH Knock-out Mice.

Cui Cao¹, Wen J. Wang¹, Ying Y. Huang¹, Hong L. Yao¹, Louis P. Conway¹, Li Liu¹, Josef Voglmeir¹

¹Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University

We describe a HPLC-based method for the determination of N-acetylneuraminic acid and N-glycolylenuraminic acid in mouse liver and milk.

Neuroscience

In Vitro Characterization of the Electrophysiological Properties of Colonic Afferent Fibers in Rats

Youqiang Meng¹, Li Dong¹, Biying Sun¹, Ping Luo¹, Guohua Zhang¹, Weifang Rong¹

¹Hongqiao International Institute of Medical Research, Tongren Hospital and Department of Physiology, Faculty of Basic Medical Sciences, Shanghai Jiaotong University School of Medicine

Abnormal sensory function underlies visceral pain and other symptoms of functional and inflammatory bowel diseases. A protocol for the electrophysiological recording of the colonic afferent nerves in an ex vivo rat colorectum preparation is presented here.

Bioengineering

Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array

Yasumasa Mashimo^1,2, Momoko Yoshioka¹, Yumie Tokunaga¹, Christopher Fockenberg¹, Shiho Terada¹, Yoshie Koyama¹, Teiko Shibata-Seki², Koki Yoshimoto¹, Risako Sakai¹, Hayase Hakariya¹, Li Liu¹, Toshihiro Akaike³, Eiry Kobatake², Siew-Eng How⁴, Motonari Uesugi^1,5, Yong Chen^1,6, Ken-ichiro Kamei¹

¹Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, ²Department of Life Science and Technology, School of Life Science and Technology, Tokyo Institute of Technology, ³Biomaterials Center for Regenerative Medical Engineering, Foundation for Advancement of International Science, ⁴Faculty of Science and Natural Resources, Universiti Malaysia Sabah, ⁵Institute for Chemical Research, Kyoto University, ⁶Ecole Normale Supérieure

This article describes the detailed methodology to prepare a Multiplexed Artificial Cellular MicroEnvironment (MACME) array for high-throughput manipulation of physical and chemical cues mimicking in vivo cellular microenvironments and to identify the optimal cellular environment for human pluripotent stem cells (hPSCs) with single-cell profiling.

Biochemistry

Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H⁺

Ya-Min Du¹, Shen-Li Zheng¹, Li Liu¹, Josef Voglmeir¹, Gabriel Yedid²

¹Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, ²College of Life Science, Nanjing Agricultural University

We describe a simple and rapid method for the preparation and analysis of N-glycans from different cultivars of radish (Raphanus sativus).

Cancer Research

Digital Analysis of Immunostaining of ZW10 Interacting Protein in Human Lung Tissues

Yuan Wen¹, Xie Song-ping², Liu Pan³, Liu Xiao-yan¹, Pan Shan⁴, Yin Qian⁴, Sun Meng⁵, Huang Xiao-xing⁶, Xiao Rui-jing⁴, Xiong Jie⁴, Zhang Qiu-ping⁴, Shao Liang¹

¹Department of Hematology, Zhongnan Hospital of Wuhan University, ²Department of Thoracic Surgery, Renmin Hospital of Wuhan University, ³Department of Human Anatomy, Histology and Embryology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, ⁴Department of Immunology, School of Basic Medical Sciences, Wuhan University, ⁵Department of Gastroenterology, Central Hospital of Wuhan, ⁶Department of Transfusion, Zhongnan Hospital of Wuhan University

ZW10 interacting protein (ZWINT) participates in the mitotic spindle checkpoint and the pathogenesis of carcinoma. Here, we introduce a methodology of the immunostaining of ZWINT in human lung cancer tissues, followed by the digital scanning of whole slides and image analysis. This methodology can provide high-quality digital images and reliable results.

Bioengineering

A Converging Strategy for the Generation of a Virtually Sequenced cDNA Library from Unreferenced Pacific Oysters

Yong M. Lyu¹, Yu Q. Li¹, Hui B. Song¹, Juan Guo¹, Ting Wang¹, Li Liu¹, Gabriel Yedid², Josef Voglmeir¹

¹Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, ²College of Life Science, Nanjing Agricultural University

We describe a strategy for how to use RNA samples from unreferenced Pacific oyster specimens, and evaluate the genetic material by comparison with publicly available genome data to generate a virtually sequenced cDNA library.

Medicine

Interphase Fluorescence in situ Hybridization of Bone Marrow Smears of Multiple Myeloma

Yalan Yu^*1, Hui Shen^*1, Li Liu¹, Ping Luo¹, Sanyun Wu¹, Jing He^1,2, Xiqin Tong¹, Yufeng Shang¹, Liang Shao¹, Fuling Zhou¹

¹Department of Hematology, Zhongnan Hospital of Wuhan University, ²Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology

Here, we present a protocol for improving the success of interphase fluorescence in situ hybridization detection on bone marrow smears from multiple myeloma patients.