The University of California Los Angeles, Los Angeles
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation
Hon Wai Koon 1, Samantha Ho 1, Michelle Cheng 1, Ryan Ichikawa 1, Charalabos Pothoulakis 1
1Center for Inflammatory Bowel Diseases, Division of Digestive Diseases, David Geffen School of Medicine, The University of California Los Angeles, Los Angeles
Bone marrow transplantation provides a way to change the genotype of the bone marrow derived cells. If the gene of interest is expressed in both bone marrow derived cells and non-bone marrow derived cells, bone marrow transplantation can change the bone marrow derived cells to a different genotype without changing the non-bone marrow derived cell genotype.
Genetics
Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
Carl Robert Rankin 1, Janet Treger 1, Emmanuelle Faure-Kumar 2, Jihane Benhammou 1, Deborah Anisman-Posner 3, Alex Edward Bollinger 3, Charalabos Pothoulakis 1, David Miguel Padua 1
1Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, University of California, Los Angeles, 2Vatche and Tamar Manoukian Division of Digestive Diseases, Integrated Molecular Technologies (IMT) Core, Department of Medicine, University of California, Los Angeles, 3Division of Hematology and Oncology, Department of Medicine, University of California, Los Angeles
Traditional cDNA-based overexpression techniques have a limited applicability for the overexpression of long noncoding RNAs due to their multiple splice forms with potential functionality. This review reports a protocol using CRISPR technology to overexpress multiple splice variants of a long noncoding RNA.
