The University of California Los Angeles, Los Angeles

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation

Hon Wai Koon¹, Samantha Ho¹, Michelle Cheng¹, Ryan Ichikawa¹, Charalabos Pothoulakis¹

¹Center for Inflammatory Bowel Diseases, Division of Digestive Diseases, David Geffen School of Medicine, The University of California Los Angeles, Los Angeles

Bone marrow transplantation provides a way to change the genotype of the bone marrow derived cells. If the gene of interest is expressed in both bone marrow derived cells and non-bone marrow derived cells, bone marrow transplantation can change the bone marrow derived cells to a different genotype without changing the non-bone marrow derived cell genotype.

Genetics

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Carl Robert Rankin¹, Janet Treger¹, Emmanuelle Faure-Kumar², Jihane Benhammou¹, Deborah Anisman-Posner³, Alex Edward Bollinger³, Charalabos Pothoulakis¹, David Miguel Padua¹

¹Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, University of California, Los Angeles, ²Vatche and Tamar Manoukian Division of Digestive Diseases, Integrated Molecular Technologies (IMT) Core, Department of Medicine, University of California, Los Angeles, ³Division of Hematology and Oncology, Department of Medicine, University of California, Los Angeles

Traditional cDNA-based overexpression techniques have a limited applicability for the overexpression of long noncoding RNAs due to their multiple splice forms with potential functionality. This review reports a protocol using CRISPR technology to overexpress multiple splice variants of a long noncoding RNA.