Naval Medical University

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

Lingzhi Fan^*1, Yue Luo^*2, Wannian Yan¹, Huanxing Han³, Pengfei Zhang¹

¹Department of Central Laboratory, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, ²Department of Dermatology, Shanghai Skin Disease Hospital, Institute of Dermatology, School of Medicine, Tongji University, ³Department of Pharmacy, Changzheng Hospital, Naval Medical University

Here, we describe a protocol for the preparation of quantum dot nanobeads (QDNB) and the detection of disease biomarkers using QDNB-based lateral flow immunoassay strips. The test results can be qualitatively assessed under UV light illumination and quantitatively measured using a fluorescent strip reader within 15 min.